A Dibasic Amino Acid Pair Conserved in the Activation Loop Directs Plasma Membrane Localization and Is Necessary for Activity of Plant Type I/II Phosphatidylinositol Phosphate Kinase
(2010) In Plant Physiology 153(3). p.1004-1015- Abstract
- Phosphatidylinositol phosphate kinase (PIPK) is an enzyme involved in the regulation of cellular levels of phosphoinositides involved in various physiological processes, such as cytoskeletal organization, ion channel activation, and vesicle trafficking. In animals, research has focused on the modes of activation and function of PIPKs, providing an understanding of the importance of plasma membrane localization. However, it still remains unclear how this issue is regulated in plant PIPKs. Here, we demonstrate that the carboxyl-terminal catalytic domain, which contains the activation loop, is sufficient for plasma membrane localization of PpPIPK1, a type I/II B PIPK from the moss Physcomitrella patens. The importance of the carboxyl-terminal... (More)
- Phosphatidylinositol phosphate kinase (PIPK) is an enzyme involved in the regulation of cellular levels of phosphoinositides involved in various physiological processes, such as cytoskeletal organization, ion channel activation, and vesicle trafficking. In animals, research has focused on the modes of activation and function of PIPKs, providing an understanding of the importance of plasma membrane localization. However, it still remains unclear how this issue is regulated in plant PIPKs. Here, we demonstrate that the carboxyl-terminal catalytic domain, which contains the activation loop, is sufficient for plasma membrane localization of PpPIPK1, a type I/II B PIPK from the moss Physcomitrella patens. The importance of the carboxyl-terminal catalytic domain for plasma membrane localization was confirmed with Arabidopsis (Arabidopsis thaliana) AtPIP5K1. Our findings, in which substitution of a conserved dibasic amino acid pair in the activation loop of PpPIPK1 completely prevented plasma membrane targeting and abolished enzymatic activity, demonstrate its critical role in these processes. Placing our results in the context of studies of eukaryotic PIPKs led us to conclude that the function of the dibasic amino acid pair in the activation loop in type I/II PIPKs is plant specific. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1628709
- author
- Mikami, Koji ; Saavedra, Laura LU ; Hiwatashi, Yuji ; Uji, Toshiki ; Hasebe, Mitsuyasu and Sommarin, Marianne LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Plant Physiology
- volume
- 153
- issue
- 3
- pages
- 1004 - 1015
- publisher
- American Society of Plant Biologists
- external identifiers
-
- wos:000279400200010
- scopus:77954305924
- pmid:20427464
- ISSN
- 1532-2548
- DOI
- 10.1104/pp.109.152686
- language
- English
- LU publication?
- yes
- id
- e5b4644f-c1a6-4161-9430-73599912b3b3 (old id 1628709)
- date added to LUP
- 2016-04-01 10:37:48
- date last changed
- 2022-03-27 18:07:16
@article{e5b4644f-c1a6-4161-9430-73599912b3b3, abstract = {{Phosphatidylinositol phosphate kinase (PIPK) is an enzyme involved in the regulation of cellular levels of phosphoinositides involved in various physiological processes, such as cytoskeletal organization, ion channel activation, and vesicle trafficking. In animals, research has focused on the modes of activation and function of PIPKs, providing an understanding of the importance of plasma membrane localization. However, it still remains unclear how this issue is regulated in plant PIPKs. Here, we demonstrate that the carboxyl-terminal catalytic domain, which contains the activation loop, is sufficient for plasma membrane localization of PpPIPK1, a type I/II B PIPK from the moss Physcomitrella patens. The importance of the carboxyl-terminal catalytic domain for plasma membrane localization was confirmed with Arabidopsis (Arabidopsis thaliana) AtPIP5K1. Our findings, in which substitution of a conserved dibasic amino acid pair in the activation loop of PpPIPK1 completely prevented plasma membrane targeting and abolished enzymatic activity, demonstrate its critical role in these processes. Placing our results in the context of studies of eukaryotic PIPKs led us to conclude that the function of the dibasic amino acid pair in the activation loop in type I/II PIPKs is plant specific.}}, author = {{Mikami, Koji and Saavedra, Laura and Hiwatashi, Yuji and Uji, Toshiki and Hasebe, Mitsuyasu and Sommarin, Marianne}}, issn = {{1532-2548}}, language = {{eng}}, number = {{3}}, pages = {{1004--1015}}, publisher = {{American Society of Plant Biologists}}, series = {{Plant Physiology}}, title = {{A Dibasic Amino Acid Pair Conserved in the Activation Loop Directs Plasma Membrane Localization and Is Necessary for Activity of Plant Type I/II Phosphatidylinositol Phosphate Kinase}}, url = {{http://dx.doi.org/10.1104/pp.109.152686}}, doi = {{10.1104/pp.109.152686}}, volume = {{153}}, year = {{2010}}, }