Transgenic overexpression of laminin 1 chain in laminin 2 chain-deficient mice rescues the disease throughout the lifespan
(2010) In Muscle and Nerve 42(1). p.30-37- Abstract
- Several approaches to treat laminin alpha 2 chain-deficient congenital muscular dystrophy (MDC1A) in mouse models have been undertaken. Most have shown promising results in young animals. However, older animals have only been characterized to some extent. Herein we analyze the lifespan of laminin alpha 2 chain deficient mice with transgenic overexpression of laminin alpha 1 chain. Further outcome measures included internalized myonuclei, heart fibrosis, grip strength, and serum creatine kinase activity. We show that laminin alpha 2-chain-deficient animals that overexpress laminin alpha 1 chain survive to up to 1.5-2 years of age. Furthermore, they displayed improved skeletal and heart muscle morphology, near-normal muscle strength, and... (More)
- Several approaches to treat laminin alpha 2 chain-deficient congenital muscular dystrophy (MDC1A) in mouse models have been undertaken. Most have shown promising results in young animals. However, older animals have only been characterized to some extent. Herein we analyze the lifespan of laminin alpha 2 chain deficient mice with transgenic overexpression of laminin alpha 1 chain. Further outcome measures included internalized myonuclei, heart fibrosis, grip strength, and serum creatine kinase activity. We show that laminin alpha 2-chain-deficient animals that overexpress laminin alpha 1 chain survive to up to 1.5-2 years of age. Furthermore, they displayed improved skeletal and heart muscle morphology, near-normal muscle strength, and normalized creatine kinase levels. Such an improvement of the dystrophic phenotype that persists to old age has not been previously demonstrated in mice. Our findings hold promise with regard to the efficient treatment of MDC1A patients in the future. Muscle Nerve 42: 30-37, 2010 (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1628963
- author
- Gawlik, Kinga LU and Durbeej-Hjalt, Madeleine LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- muscular dystrophy, heart, gene therapy, muscle, laminin
- in
- Muscle and Nerve
- volume
- 42
- issue
- 1
- pages
- 30 - 37
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000279456400005
- scopus:77954104087
- pmid:20544910
- ISSN
- 0148-639X
- DOI
- 10.1002/mus.21616
- language
- English
- LU publication?
- yes
- id
- 6adaf207-c499-4774-a6d1-8a10b0b2cd14 (old id 1628963)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20544910?dopt=Abstract
- date added to LUP
- 2016-04-01 10:11:30
- date last changed
- 2022-01-25 20:42:39
@article{6adaf207-c499-4774-a6d1-8a10b0b2cd14,
abstract = {{Several approaches to treat laminin alpha 2 chain-deficient congenital muscular dystrophy (MDC1A) in mouse models have been undertaken. Most have shown promising results in young animals. However, older animals have only been characterized to some extent. Herein we analyze the lifespan of laminin alpha 2 chain deficient mice with transgenic overexpression of laminin alpha 1 chain. Further outcome measures included internalized myonuclei, heart fibrosis, grip strength, and serum creatine kinase activity. We show that laminin alpha 2-chain-deficient animals that overexpress laminin alpha 1 chain survive to up to 1.5-2 years of age. Furthermore, they displayed improved skeletal and heart muscle morphology, near-normal muscle strength, and normalized creatine kinase levels. Such an improvement of the dystrophic phenotype that persists to old age has not been previously demonstrated in mice. Our findings hold promise with regard to the efficient treatment of MDC1A patients in the future. Muscle Nerve 42: 30-37, 2010}},
author = {{Gawlik, Kinga and Durbeej-Hjalt, Madeleine}},
issn = {{0148-639X}},
keywords = {{muscular dystrophy; heart; gene therapy; muscle; laminin}},
language = {{eng}},
number = {{1}},
pages = {{30--37}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Muscle and Nerve}},
title = {{Transgenic overexpression of laminin 1 chain in laminin 2 chain-deficient mice rescues the disease throughout the lifespan}},
url = {{http://dx.doi.org/10.1002/mus.21616}},
doi = {{10.1002/mus.21616}},
volume = {{42}},
year = {{2010}},
}