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Genetic profiles of gastroesophageal cancer: combined analysis using expression array and tiling array-comparative genomic hybridization

Isinger Ekstrand, Anna LU ; Johansson, Jan LU ; Ohlsson, Mattias LU ; Francis, Princy LU ; Staaf, Johan LU ; Jönsson, Mats LU ; Borg, Åke LU and Nilbert, Mef LU (2010) In Cancer Genetics and Cytogenetics1979-01-01+01:002011-01-01+01:00 200(2). p.120-126
Abstract
We aimed to characterize the genomic profiles of adenocarcinomas in the gastroesophageal junction in relation to cancers in the esophagus and the stomach. Profiles of gains/losses as well as gene expression profiles were obtained from 27 gastroesophageal adenocarcinomas by means of 32k high-resolution array-based comparative genomic hybridization and 27k oligo gene expression arrays, and putative target genes were validated in an extended series. Adenocarcinomas in the distal esophagus and the gastroesophageal junction showed strong similarities with the most common gains at 20q13, 8q24, 1q21-23, 5p15, 13q34, and 12q13, whereas different profiles with gains at 5p15, 7p22, 2q35, and 13q34 characterized gastric cancers. CDK6 and EGFR were... (More)
We aimed to characterize the genomic profiles of adenocarcinomas in the gastroesophageal junction in relation to cancers in the esophagus and the stomach. Profiles of gains/losses as well as gene expression profiles were obtained from 27 gastroesophageal adenocarcinomas by means of 32k high-resolution array-based comparative genomic hybridization and 27k oligo gene expression arrays, and putative target genes were validated in an extended series. Adenocarcinomas in the distal esophagus and the gastroesophageal junction showed strong similarities with the most common gains at 20q13, 8q24, 1q21-23, 5p15, 13q34, and 12q13, whereas different profiles with gains at 5p15, 7p22, 2q35, and 13q34 characterized gastric cancers. CDK6 and EGFR were identified as putative target genes in cancers of the esophagus and the gastroesophageal junction, with upregulation in one quarter of the tumors. Gains/losses and gene expression profiles show strong similarity between cancers in the distal esophagus and the gastroesophageal junction with frequent upregulation of CDK6 and EGFR, whereas gastric cancer displays distinct genetic changes. These data suggest that molecular diagnostics and targeted therapies can be applied to adenocarcinomas of the distal esophagus and gastroesophageal junction alike. (C) 2010 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Esophageal Neoplasms: genetics, Oligonucleotide Array Sequence Analysis: methods, Comparative Genomic Hybridization: methods, Cyclin-Dependent Kinase 6: genetics, Receptor, Epidermal Growth Factor: genetics, Stomach Neoplasms: genetics
in
Cancer Genetics and Cytogenetics1979-01-01+01:002011-01-01+01:00
volume
200
issue
2
pages
120 - 126
publisher
Elsevier
external identifiers
  • wos:000279373500006
  • scopus:77953805124
ISSN
0165-4608
DOI
10.1016/j.cancergencyto.2010.03.013
language
English
LU publication?
yes
id
b6fc8bb0-5f9b-42a8-a1ed-b9d72e230798 (old id 1629330)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20620594?dopt=Abstract
date added to LUP
2010-07-22 11:05:06
date last changed
2018-05-29 10:24:02
@article{b6fc8bb0-5f9b-42a8-a1ed-b9d72e230798,
  abstract     = {We aimed to characterize the genomic profiles of adenocarcinomas in the gastroesophageal junction in relation to cancers in the esophagus and the stomach. Profiles of gains/losses as well as gene expression profiles were obtained from 27 gastroesophageal adenocarcinomas by means of 32k high-resolution array-based comparative genomic hybridization and 27k oligo gene expression arrays, and putative target genes were validated in an extended series. Adenocarcinomas in the distal esophagus and the gastroesophageal junction showed strong similarities with the most common gains at 20q13, 8q24, 1q21-23, 5p15, 13q34, and 12q13, whereas different profiles with gains at 5p15, 7p22, 2q35, and 13q34 characterized gastric cancers. CDK6 and EGFR were identified as putative target genes in cancers of the esophagus and the gastroesophageal junction, with upregulation in one quarter of the tumors. Gains/losses and gene expression profiles show strong similarity between cancers in the distal esophagus and the gastroesophageal junction with frequent upregulation of CDK6 and EGFR, whereas gastric cancer displays distinct genetic changes. These data suggest that molecular diagnostics and targeted therapies can be applied to adenocarcinomas of the distal esophagus and gastroesophageal junction alike. (C) 2010 Elsevier Inc. All rights reserved.},
  author       = {Isinger Ekstrand, Anna and Johansson, Jan and Ohlsson, Mattias and Francis, Princy and Staaf, Johan and Jönsson, Mats and Borg, Åke and Nilbert, Mef},
  issn         = {0165-4608},
  keyword      = {Esophageal Neoplasms: genetics,Oligonucleotide Array Sequence Analysis: methods,Comparative Genomic Hybridization: methods,Cyclin-Dependent Kinase 6: genetics,Receptor,Epidermal Growth Factor: genetics,Stomach Neoplasms: genetics},
  language     = {eng},
  number       = {2},
  pages        = {120--126},
  publisher    = {Elsevier},
  series       = {Cancer Genetics and Cytogenetics1979-01-01+01:002011-01-01+01:00},
  title        = {Genetic profiles of gastroesophageal cancer: combined analysis using expression array and tiling array-comparative genomic hybridization},
  url          = {http://dx.doi.org/10.1016/j.cancergencyto.2010.03.013},
  volume       = {200},
  year         = {2010},
}