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Plasma volume expansion of 5% albumin, 4% gelatin, 6% HES 130/0.4, and normal saline under increased microvascular permeability in the rat.

Dubniks, Maris LU ; Persson, Johan LU and Grände, Per-Olof LU (2007) In Intensive Care Medicine 33(2). p.293-299
Abstract
Objective: To compare the colloids 5% albumin, 4% gelatin, and 6% HES 130/0.4 with one another and with normal saline regarding their plasma expanding effects at increased permeability and to compare the results with those from a previous study at normal permeability. Design and setting: Prospective controlled randomized laboratory study in a university research laboratory. Subjects: 48 adult male Sprague-Dawley rats. Interventions: Permeability was increased by an injection of 0.5 ml dextran 70 using the fact that dextran causes anaphylactic reaction in the rat. Plasma volume was determined (I-125 albumin tracer technique) after anesthesia, 1 h after dextran injection (before infusion for 10-15 min of 20 ml/kg bw of each of the colloids... (More)
Objective: To compare the colloids 5% albumin, 4% gelatin, and 6% HES 130/0.4 with one another and with normal saline regarding their plasma expanding effects at increased permeability and to compare the results with those from a previous study at normal permeability. Design and setting: Prospective controlled randomized laboratory study in a university research laboratory. Subjects: 48 adult male Sprague-Dawley rats. Interventions: Permeability was increased by an injection of 0.5 ml dextran 70 using the fact that dextran causes anaphylactic reaction in the rat. Plasma volume was determined (I-125 albumin tracer technique) after anesthesia, 1 h after dextran injection (before infusion for 10-15 min of 20 ml/kg bw of each of the colloids or 80 ml/kg saline), and 3 h later. Blood pressure, hematocrit, blood gases, and electrolytes were measured. CVP was measured in four rats. Measurements and results: Plasma volume was 41.1 +/- 1.9 ml/kg at baseline (n = 9), and 29.1 +/- 4.1 ml/kg (n = 35) 1 h after the dextran injection. Three hours after infusion of the plasma expander plasma volume had increased by 17.1 +/- 3.4 ml/kg in the albumin group, 7.9 +/- 3.6 ml/kg in the gelatin group, 7.4 +/- 4.4 ml/kg in the HES group, and 12.2 +/- 3.1 ml/kg in the saline group. It was unchanged in a control group given no solution (n = 7 for all groups). Conclusion: Albumin was a more effective plasma volume expander than gelatin or HES or saline (saline in 4 times larger volume). Gelatin and HES were equally effective. All solutions showed a smaller plasma expanding effect than observed in a previous study with normal permeability. (Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
plasma volume, transcapillary fluid exchange, vascular permeability, colloids, crystalloids
in
Intensive Care Medicine
volume
33
issue
2
pages
293 - 299
publisher
Springer Science and Business Media B.V.
external identifiers
  • wos:000244026200013
  • scopus:33846889952
ISSN
0342-4642
DOI
10.1007/s00134-006-0454-5
language
English
LU publication?
yes
id
17852f98-09b0-45b2-a0b6-c87ffd64ab8f (old id 163041)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17119921&dopt=Abstract
date added to LUP
2016-04-01 12:12:13
date last changed
2025-01-02 09:28:26
@article{17852f98-09b0-45b2-a0b6-c87ffd64ab8f,
  abstract     = {{Objective: To compare the colloids 5% albumin, 4% gelatin, and 6% HES 130/0.4 with one another and with normal saline regarding their plasma expanding effects at increased permeability and to compare the results with those from a previous study at normal permeability. Design and setting: Prospective controlled randomized laboratory study in a university research laboratory. Subjects: 48 adult male Sprague-Dawley rats. Interventions: Permeability was increased by an injection of 0.5 ml dextran 70 using the fact that dextran causes anaphylactic reaction in the rat. Plasma volume was determined (I-125 albumin tracer technique) after anesthesia, 1 h after dextran injection (before infusion for 10-15 min of 20 ml/kg bw of each of the colloids or 80 ml/kg saline), and 3 h later. Blood pressure, hematocrit, blood gases, and electrolytes were measured. CVP was measured in four rats. Measurements and results: Plasma volume was 41.1 +/- 1.9 ml/kg at baseline (n = 9), and 29.1 +/- 4.1 ml/kg (n = 35) 1 h after the dextran injection. Three hours after infusion of the plasma expander plasma volume had increased by 17.1 +/- 3.4 ml/kg in the albumin group, 7.9 +/- 3.6 ml/kg in the gelatin group, 7.4 +/- 4.4 ml/kg in the HES group, and 12.2 +/- 3.1 ml/kg in the saline group. It was unchanged in a control group given no solution (n = 7 for all groups). Conclusion: Albumin was a more effective plasma volume expander than gelatin or HES or saline (saline in 4 times larger volume). Gelatin and HES were equally effective. All solutions showed a smaller plasma expanding effect than observed in a previous study with normal permeability.}},
  author       = {{Dubniks, Maris and Persson, Johan and Grände, Per-Olof}},
  issn         = {{0342-4642}},
  keywords     = {{plasma volume; transcapillary fluid exchange; vascular permeability; colloids; crystalloids}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{293--299}},
  publisher    = {{Springer Science and Business Media B.V.}},
  series       = {{Intensive Care Medicine}},
  title        = {{Plasma volume expansion of 5% albumin, 4% gelatin, 6% HES 130/0.4, and normal saline under increased microvascular permeability in the rat.}},
  url          = {{https://lup.lub.lu.se/search/files/2825264/625748.pdf}},
  doi          = {{10.1007/s00134-006-0454-5}},
  volume       = {{33}},
  year         = {{2007}},
}