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Modeling the Time Evolution of the Nanoparticle-Protein Corona in a Body Fluid

Dell'Orco, Daniele ; Lundqvist, Martin LU ; Oslakovic, Cecilia LU ; Cedervall, Tommy LU and Linse, Sara LU (2010) In PLoS ONE 5.
Abstract
Background: Nanoparticles in contact with biological fluids interact with proteins and other biomolecules, thus forming a dynamic corona whose composition varies over time due to continuous protein association and dissociation events. Eventually equilibrium is reached, at which point the continued exchange will not affect the composition of the corona. Results: We developed a simple and effective dynamic model of the nanoparticle protein corona in a body fluid, namely human plasma. The model predicts the time evolution and equilibrium composition of the corona based on affinities, stoichiometries and rate constants. An application to the interaction of human serum albumin, high density lipoprotein (HDL) and fibrinogen with 70 nm... (More)
Background: Nanoparticles in contact with biological fluids interact with proteins and other biomolecules, thus forming a dynamic corona whose composition varies over time due to continuous protein association and dissociation events. Eventually equilibrium is reached, at which point the continued exchange will not affect the composition of the corona. Results: We developed a simple and effective dynamic model of the nanoparticle protein corona in a body fluid, namely human plasma. The model predicts the time evolution and equilibrium composition of the corona based on affinities, stoichiometries and rate constants. An application to the interaction of human serum albumin, high density lipoprotein (HDL) and fibrinogen with 70 nm N-iso-propylacrylamide/N-tert-butylacrylamide copolymer nanoparticles is presented, including novel experimental data for HDL. Conclusions: The simple model presented here can easily be modified to mimic the interaction of the nanoparticle protein corona with a novel biological fluid or compartment once new data will be available, thus opening novel applications in nanotoxicity and nanomedicine. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
5
publisher
Public Library of Science (PLoS)
external identifiers
  • wos:000278318700018
  • scopus:77956215287
  • pmid:20532175
ISSN
1932-6203
DOI
10.1371/journal.pone.0010949
language
English
LU publication?
yes
id
22cbdcba-4293-4634-bd53-d36fd64e78b3 (old id 1631667)
date added to LUP
2016-04-01 14:59:32
date last changed
2023-11-13 14:27:07
@article{22cbdcba-4293-4634-bd53-d36fd64e78b3,
  abstract     = {{Background: Nanoparticles in contact with biological fluids interact with proteins and other biomolecules, thus forming a dynamic corona whose composition varies over time due to continuous protein association and dissociation events. Eventually equilibrium is reached, at which point the continued exchange will not affect the composition of the corona. Results: We developed a simple and effective dynamic model of the nanoparticle protein corona in a body fluid, namely human plasma. The model predicts the time evolution and equilibrium composition of the corona based on affinities, stoichiometries and rate constants. An application to the interaction of human serum albumin, high density lipoprotein (HDL) and fibrinogen with 70 nm N-iso-propylacrylamide/N-tert-butylacrylamide copolymer nanoparticles is presented, including novel experimental data for HDL. Conclusions: The simple model presented here can easily be modified to mimic the interaction of the nanoparticle protein corona with a novel biological fluid or compartment once new data will be available, thus opening novel applications in nanotoxicity and nanomedicine.}},
  author       = {{Dell'Orco, Daniele and Lundqvist, Martin and Oslakovic, Cecilia and Cedervall, Tommy and Linse, Sara}},
  issn         = {{1932-6203}},
  language     = {{eng}},
  publisher    = {{Public Library of Science (PLoS)}},
  series       = {{PLoS ONE}},
  title        = {{Modeling the Time Evolution of the Nanoparticle-Protein Corona in a Body Fluid}},
  url          = {{http://dx.doi.org/10.1371/journal.pone.0010949}},
  doi          = {{10.1371/journal.pone.0010949}},
  volume       = {{5}},
  year         = {{2010}},
}