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A population of tRNA-derived small RNAs is actively produced in Trypanosoma cruzi and recruited to specific cytoplasmic granules

Garcia-Silva, Maria Rosa; Frugier, Magali; Tosar, Juan Pablo; Correa-Dominguez, Alejandro; Ronalte-Alves, Lysangela; Parodi-Talice, Adriana; Rovira, Carlos LU ; Robello, Carlos; Goldenberg, Samuel and Cayota, Alfonso (2010) In Molecular and Biochemical Parasitology 171(2). p.64-73
Abstract
Over the last years an expanding family of small RNAs (i.e. microRNAs, siRNAs and piRNAs) was recognized as key players in diverse forms of gene silencing and chromatin organization. Effectors functions of these small RNAs are achieved through ribonucleoprotein (RNP) complexes containing at their center an Argonaute/Piwi protein. Although these proteins and their small RNA-associated machinery can be traced back to the common ancestor of eukaryotes, this machinery seems to be entirely lost or extensively simplified in some unicellular organisms including Trypanosoma cruzi, which are unable to trigger RNAi related phenomena. Speculating about the presence of alternate small RNA-mediated pathways in these organisms, we constructed and... (More)
Over the last years an expanding family of small RNAs (i.e. microRNAs, siRNAs and piRNAs) was recognized as key players in diverse forms of gene silencing and chromatin organization. Effectors functions of these small RNAs are achieved through ribonucleoprotein (RNP) complexes containing at their center an Argonaute/Piwi protein. Although these proteins and their small RNA-associated machinery can be traced back to the common ancestor of eukaryotes, this machinery seems to be entirely lost or extensively simplified in some unicellular organisms including Trypanosoma cruzi, which are unable to trigger RNAi related phenomena. Speculating about the presence of alternate small RNA-mediated pathways in these organisms, we constructed and analyzed a size-fractionated cDNA library (20-35 nt) from epimastigotes forms of T. cruzi. Our results showed the production of an abundant class of tRNA-derived small RNAs preferentially restricted to specific isoacceptors and whose production was more accentuated under nutritional stress. These small tRNAs derived preferentially from the 5' halves of mature tRNAs and were recruited to distinctive cytoplasmic granules. Our data favor the idea that tRNA cleavage is unlikely to be the consequence of non-specific degradation but a controlled process, whose biological significance remains to be elucidated. (C) 2010 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Trypanosoma cruzi, tRNA, Small non-coding RNAs
in
Molecular and Biochemical Parasitology
volume
171
issue
2
pages
64 - 73
publisher
Elsevier
external identifiers
  • wos:000278306900002
  • scopus:77951937781
ISSN
1872-9428
DOI
10.1016/j.molbiopara.2010.02.003
language
English
LU publication?
yes
id
cd5775e7-6d1d-41ce-8ef5-8e5a7a83d47c (old id 1631783)
date added to LUP
2010-07-22 12:22:27
date last changed
2018-06-10 03:29:49
@article{cd5775e7-6d1d-41ce-8ef5-8e5a7a83d47c,
  abstract     = {Over the last years an expanding family of small RNAs (i.e. microRNAs, siRNAs and piRNAs) was recognized as key players in diverse forms of gene silencing and chromatin organization. Effectors functions of these small RNAs are achieved through ribonucleoprotein (RNP) complexes containing at their center an Argonaute/Piwi protein. Although these proteins and their small RNA-associated machinery can be traced back to the common ancestor of eukaryotes, this machinery seems to be entirely lost or extensively simplified in some unicellular organisms including Trypanosoma cruzi, which are unable to trigger RNAi related phenomena. Speculating about the presence of alternate small RNA-mediated pathways in these organisms, we constructed and analyzed a size-fractionated cDNA library (20-35 nt) from epimastigotes forms of T. cruzi. Our results showed the production of an abundant class of tRNA-derived small RNAs preferentially restricted to specific isoacceptors and whose production was more accentuated under nutritional stress. These small tRNAs derived preferentially from the 5' halves of mature tRNAs and were recruited to distinctive cytoplasmic granules. Our data favor the idea that tRNA cleavage is unlikely to be the consequence of non-specific degradation but a controlled process, whose biological significance remains to be elucidated. (C) 2010 Elsevier B.V. All rights reserved.},
  author       = {Garcia-Silva, Maria Rosa and Frugier, Magali and Tosar, Juan Pablo and Correa-Dominguez, Alejandro and Ronalte-Alves, Lysangela and Parodi-Talice, Adriana and Rovira, Carlos and Robello, Carlos and Goldenberg, Samuel and Cayota, Alfonso},
  issn         = {1872-9428},
  keyword      = {Trypanosoma cruzi,tRNA,Small non-coding RNAs},
  language     = {eng},
  number       = {2},
  pages        = {64--73},
  publisher    = {Elsevier},
  series       = {Molecular and Biochemical Parasitology},
  title        = {A population of tRNA-derived small RNAs is actively produced in Trypanosoma cruzi and recruited to specific cytoplasmic granules},
  url          = {http://dx.doi.org/10.1016/j.molbiopara.2010.02.003},
  volume       = {171},
  year         = {2010},
}