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Proteomic analysis identifies candidate proteins associated with distant recurrences in breast cancer after adjuvant chemotherapy.

Niméus, Emma LU ; Malmström, Johan LU ; Johnsson, Anders LU ; Marko-Varga, György LU and Fernö, Mårten LU (2007) In Journal of Pharmaceutical and Biomedical Analysis 43(3). p.1086-1093
Abstract
Breast cancer is a heterogenous disease and it is of importance to select patients with regard to different prognosis and treatment sensitivity to individualize treatment regimes. In this study we successfully adapted a protein extraction protocol from mRNA extracted tumor samples enabling two-dimensional gel electrophoresis (2-DE) analysis of samples previously analyzed by cDNA microarray. The aim was to find candidate proteins that distinguish breast cancer patients with or without recurrences after adjuvant CMF (cyclophosphamide, methotrexate and 5-FU) treatment within four years to follow-up. We identified several proteins distinguishing the recurrence group from the non-recurrence group, especially in the ER and PgR positive subgroup... (More)
Breast cancer is a heterogenous disease and it is of importance to select patients with regard to different prognosis and treatment sensitivity to individualize treatment regimes. In this study we successfully adapted a protein extraction protocol from mRNA extracted tumor samples enabling two-dimensional gel electrophoresis (2-DE) analysis of samples previously analyzed by cDNA microarray. The aim was to find candidate proteins that distinguish breast cancer patients with or without recurrences after adjuvant CMF (cyclophosphamide, methotrexate and 5-FU) treatment within four years to follow-up. We identified several proteins distinguishing the recurrence group from the non-recurrence group, especially in the ER and PgR positive subgroup (n = 7). The induced proteins were involved in translation/folding, iron ion binding, and protease inhibition, whereas proteins involved in signaling, ubiquitination, and splicing were decreased in expression. These results show that it is possible to use 2-DE to separate high abundant proteins in breast cancer tissue and to find discriminating proteins to identify patients with different prognosis after adjuvant CMF treatment. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
drug resistance, breast cancer, prognostic markers, two-dimensional gel electrophoresis
in
Journal of Pharmaceutical and Biomedical Analysis
volume
43
issue
3
pages
1086 - 1093
publisher
Elsevier
external identifiers
  • wos:000244623000040
  • scopus:33846593395
ISSN
0731-7085
DOI
10.1016/j.jpba.2006.09.019
language
English
LU publication?
yes
id
ca1accd0-43aa-44f8-8fa7-d776f495f06e (old id 163421)
date added to LUP
2007-06-27 13:32:29
date last changed
2017-08-27 03:57:06
@article{ca1accd0-43aa-44f8-8fa7-d776f495f06e,
  abstract     = {Breast cancer is a heterogenous disease and it is of importance to select patients with regard to different prognosis and treatment sensitivity to individualize treatment regimes. In this study we successfully adapted a protein extraction protocol from mRNA extracted tumor samples enabling two-dimensional gel electrophoresis (2-DE) analysis of samples previously analyzed by cDNA microarray. The aim was to find candidate proteins that distinguish breast cancer patients with or without recurrences after adjuvant CMF (cyclophosphamide, methotrexate and 5-FU) treatment within four years to follow-up. We identified several proteins distinguishing the recurrence group from the non-recurrence group, especially in the ER and PgR positive subgroup (n = 7). The induced proteins were involved in translation/folding, iron ion binding, and protease inhibition, whereas proteins involved in signaling, ubiquitination, and splicing were decreased in expression. These results show that it is possible to use 2-DE to separate high abundant proteins in breast cancer tissue and to find discriminating proteins to identify patients with different prognosis after adjuvant CMF treatment.},
  author       = {Niméus, Emma and Malmström, Johan and Johnsson, Anders and Marko-Varga, György and Fernö, Mårten},
  issn         = {0731-7085},
  keyword      = {drug resistance,breast cancer,prognostic markers,two-dimensional gel electrophoresis},
  language     = {eng},
  number       = {3},
  pages        = {1086--1093},
  publisher    = {Elsevier},
  series       = {Journal of Pharmaceutical and Biomedical Analysis},
  title        = {Proteomic analysis identifies candidate proteins associated with distant recurrences in breast cancer after adjuvant chemotherapy.},
  url          = {http://dx.doi.org/10.1016/j.jpba.2006.09.019},
  volume       = {43},
  year         = {2007},
}