Proteomic analysis identifies candidate proteins associated with distant recurrences in breast cancer after adjuvant chemotherapy.
Niméus, Emma LU ; Malmström, Johan LU
; Johnsson, Anders
LU
; Marko-Varga, György
LU
and Fernö, Mårten
LU
(2007)
In Journal of Pharmaceutical and Biomedical Analysis
43(3).
p.1086-1093
- Abstract
- Breast cancer is a heterogenous disease and it is of importance to select patients with regard to different prognosis and treatment sensitivity to individualize treatment regimes. In this study we successfully adapted a protein extraction protocol from mRNA extracted tumor samples enabling two-dimensional gel electrophoresis (2-DE) analysis of samples previously analyzed by cDNA microarray. The aim was to find candidate proteins that distinguish breast cancer patients with or without recurrences after adjuvant CMF (cyclophosphamide, methotrexate and 5-FU) treatment within four years to follow-up. We identified several proteins distinguishing the recurrence group from the non-recurrence group, especially in the ER and PgR positive subgroup... (More)
- Breast cancer is a heterogenous disease and it is of importance to select patients with regard to different prognosis and treatment sensitivity to individualize treatment regimes. In this study we successfully adapted a protein extraction protocol from mRNA extracted tumor samples enabling two-dimensional gel electrophoresis (2-DE) analysis of samples previously analyzed by cDNA microarray. The aim was to find candidate proteins that distinguish breast cancer patients with or without recurrences after adjuvant CMF (cyclophosphamide, methotrexate and 5-FU) treatment within four years to follow-up. We identified several proteins distinguishing the recurrence group from the non-recurrence group, especially in the ER and PgR positive subgroup (n = 7). The induced proteins were involved in translation/folding, iron ion binding, and protease inhibition, whereas proteins involved in signaling, ubiquitination, and splicing were decreased in expression. These results show that it is possible to use 2-DE to separate high abundant proteins in breast cancer tissue and to find discriminating proteins to identify patients with different prognosis after adjuvant CMF treatment. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/163421
- author
- Niméus, Emma
LU
; Malmström, Johan
LU
; Johnsson, Anders
LU
; Marko-Varga, György
LU
and Fernö, Mårten
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- drug resistance, breast cancer, prognostic markers, two-dimensional gel electrophoresis
- in
- Journal of Pharmaceutical and Biomedical Analysis
- volume
- 43
- issue
- 3
- pages
- 1086 - 1093
- publisher
- Elsevier
- external identifiers
-
- wos:000244623000040
- scopus:33846593395
- pmid:17085005
- ISSN
- 0731-7085
- DOI
- 10.1016/j.jpba.2006.09.019
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Division V (013230900), Department of Experimental Medical Science (013210000), Oncology, MV (013035000), Lung Biology (013212002)
- id
- ca1accd0-43aa-44f8-8fa7-d776f495f06e (old id 163421)
- date added to LUP
- 2016-04-01 11:54:00
- date last changed
- 2022-03-13 02:17:40
@article{ca1accd0-43aa-44f8-8fa7-d776f495f06e,
abstract = {{Breast cancer is a heterogenous disease and it is of importance to select patients with regard to different prognosis and treatment sensitivity to individualize treatment regimes. In this study we successfully adapted a protein extraction protocol from mRNA extracted tumor samples enabling two-dimensional gel electrophoresis (2-DE) analysis of samples previously analyzed by cDNA microarray. The aim was to find candidate proteins that distinguish breast cancer patients with or without recurrences after adjuvant CMF (cyclophosphamide, methotrexate and 5-FU) treatment within four years to follow-up. We identified several proteins distinguishing the recurrence group from the non-recurrence group, especially in the ER and PgR positive subgroup (n = 7). The induced proteins were involved in translation/folding, iron ion binding, and protease inhibition, whereas proteins involved in signaling, ubiquitination, and splicing were decreased in expression. These results show that it is possible to use 2-DE to separate high abundant proteins in breast cancer tissue and to find discriminating proteins to identify patients with different prognosis after adjuvant CMF treatment.}},
author = {{Niméus, Emma and Malmström, Johan and Johnsson, Anders and Marko-Varga, György and Fernö, Mårten}},
issn = {{0731-7085}},
keywords = {{drug resistance; breast cancer; prognostic markers; two-dimensional gel electrophoresis}},
language = {{eng}},
number = {{3}},
pages = {{1086--1093}},
publisher = {{Elsevier}},
series = {{Journal of Pharmaceutical and Biomedical Analysis}},
title = {{Proteomic analysis identifies candidate proteins associated with distant recurrences in breast cancer after adjuvant chemotherapy.}},
url = {{http://dx.doi.org/10.1016/j.jpba.2006.09.019}},
doi = {{10.1016/j.jpba.2006.09.019}},
volume = {{43}},
year = {{2007}},
}