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The macrophage beta-glucan receptor mediates arachidonate release induced by zymosan: Essential role for Src family kinases.

Olsson, Sandra LU and Sundler, Roger LU (2007) In Molecular Immunology 44(7). p.1509-1515
Abstract
Yeast-derived zymosan beads are among the classical agents used to induce sterile inflammatory responses in experimental animals and macrophage activation in cell culture. In macrophages the cytosolic phospholipase A2 becomes activated, leading to mobilization of arachidonate and the generation of prostaglandins and leukotrienes. Although zymosan can interact with several receptors it has not been unequivocally demonstrated which interaction is required for induction of the eicosanoid response. We have compared arachidonate release induced in primary mouse macrophages by zymosan and particulate beta-glucan and found striking similarities. The similarities include the effects of dectin-1 antagonists (soluble beta-glucan and laminarin) and... (More)
Yeast-derived zymosan beads are among the classical agents used to induce sterile inflammatory responses in experimental animals and macrophage activation in cell culture. In macrophages the cytosolic phospholipase A2 becomes activated, leading to mobilization of arachidonate and the generation of prostaglandins and leukotrienes. Although zymosan can interact with several receptors it has not been unequivocally demonstrated which interaction is required for induction of the eicosanoid response. We have compared arachidonate release induced in primary mouse macrophages by zymosan and particulate beta-glucan and found striking similarities. The similarities include the effects of dectin-1 antagonists (soluble beta-glucan and laminarin) and of inhibitors of Src family kinases, the Tec kinase Btk, phosphatidylinositol 3-kinase and the Map kinases ERK and p38. Furthermore, particulate beta-glucan was equally effective as zymosan in causing phosphorylation of phospholipase C gamma 2, arguing that both agents act via the beta-glucan receptor dectin-1 and that the above signal components are engaged down-stream of that receptor. Suggestive evidence for a role of the scaffold adaptor Gab2 is also presented. (c) 2006 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Btk Gab2, cPLA2, dectin-1, eicosanoids, PLC gamma 2
in
Molecular Immunology
volume
44
issue
7
pages
1509 - 1515
publisher
Pergamon
external identifiers
  • wos:000243759700005
  • scopus:33751214234
ISSN
1872-9142
DOI
10.1016/j.molimm.2006.09.004
language
English
LU publication?
yes
id
ce3b64d4-831f-4a17-a252-11d9e29dab52 (old id 163439)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17084455&dopt=Abstract
date added to LUP
2007-07-18 14:44:42
date last changed
2017-01-01 06:53:50
@article{ce3b64d4-831f-4a17-a252-11d9e29dab52,
  abstract     = {Yeast-derived zymosan beads are among the classical agents used to induce sterile inflammatory responses in experimental animals and macrophage activation in cell culture. In macrophages the cytosolic phospholipase A2 becomes activated, leading to mobilization of arachidonate and the generation of prostaglandins and leukotrienes. Although zymosan can interact with several receptors it has not been unequivocally demonstrated which interaction is required for induction of the eicosanoid response. We have compared arachidonate release induced in primary mouse macrophages by zymosan and particulate beta-glucan and found striking similarities. The similarities include the effects of dectin-1 antagonists (soluble beta-glucan and laminarin) and of inhibitors of Src family kinases, the Tec kinase Btk, phosphatidylinositol 3-kinase and the Map kinases ERK and p38. Furthermore, particulate beta-glucan was equally effective as zymosan in causing phosphorylation of phospholipase C gamma 2, arguing that both agents act via the beta-glucan receptor dectin-1 and that the above signal components are engaged down-stream of that receptor. Suggestive evidence for a role of the scaffold adaptor Gab2 is also presented. (c) 2006 Elsevier Ltd. All rights reserved.},
  author       = {Olsson, Sandra and Sundler, Roger},
  issn         = {1872-9142},
  keyword      = {Btk
Gab2,cPLA2,dectin-1,eicosanoids,PLC gamma 2},
  language     = {eng},
  number       = {7},
  pages        = {1509--1515},
  publisher    = {Pergamon},
  series       = {Molecular Immunology},
  title        = {The macrophage beta-glucan receptor mediates arachidonate release induced by zymosan: Essential role for Src family kinases.},
  url          = {http://dx.doi.org/10.1016/j.molimm.2006.09.004},
  volume       = {44},
  year         = {2007},
}