Long-term neuroretinal full-thickness transplants in a large animal model of severe retinitis pigmentosa.
(2007) In Graefe's Archive for Clinical and Experimental Ophthalmology 245(6). p.835-846- Abstract
- The purpose of this study was to explore neuroretinal transplantation in a large animal model of severe retinitis pigmentosa and to establish graft development, long-term survival, graft-host integration, and effects on the host retina. Methods Rhodopsin transgenic pigs, aged 6 months, received in one eye a fetal full-thickness neuroretinal sheet in the subretinal space by means of vitrectomy and retinotomy. Six months postoperatively, eyes were studied in the light microscope and with immunohistochemical markers. Full-field electroretinography (ERG) was performed at 4 and 6 months. Results Laminated grafts with well-organized photoreceptors, rod bipolar cells, and Muller cells were found in five of six eyes. Neuronal connections between... (More)
- The purpose of this study was to explore neuroretinal transplantation in a large animal model of severe retinitis pigmentosa and to establish graft development, long-term survival, graft-host integration, and effects on the host retina. Methods Rhodopsin transgenic pigs, aged 6 months, received in one eye a fetal full-thickness neuroretinal sheet in the subretinal space by means of vitrectomy and retinotomy. Six months postoperatively, eyes were studied in the light microscope and with immunohistochemical markers. Full-field electroretinography (ERG) was performed at 4 and 6 months. Results Laminated grafts with well-organized photoreceptors, rod bipolar cells, and Muller cells were found in five of six eyes. Neuronal connections between graft and host retina were not seen. In the five eyes containing a graft, the number of surviving rods in the host retina was significantly higher compared with unoperated eyes. The ERG did not reveal any significant difference in b-wave amplitude between operated and control eyes, but the cone-derived response in operated eyes increased significantly from 4 to 6 months while the rod response in control eyes decreased significantly. Conclusions Fetal full-thickness neuroretina can be transplanted safely to an eye with severe retinal degeneration. In their major part, the transplants develop a normal laminated morphology and survive for at least 6 months. Graft and host retinal neurons do not form connections. Retinal function in the host is reduced initially by the surgical trauma, but the presence of a well-laminated graft counteracts this effect and rescues rods from degeneration. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/163560
- author
- Ghosh, Fredrik LU ; Engelsberg, Karl LU ; English, Robert and Petters, Robert
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- immune privilege, retinal degeneration, photoreceptor morphology, vitreoretinal surgery
- in
- Graefe's Archive for Clinical and Experimental Ophthalmology
- volume
- 245
- issue
- 6
- pages
- 835 - 846
- publisher
- Springer
- external identifiers
-
- wos:000246594000010
- scopus:34249296671
- ISSN
- 1435-702X
- DOI
- 10.1007/s00417-006-0437-9
- language
- English
- LU publication?
- yes
- id
- 86987cdc-6e7a-42f1-9da8-980185b34956 (old id 163560)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17072635&dopt=Abstract
- date added to LUP
- 2016-04-01 15:24:26
- date last changed
- 2022-01-28 05:10:43
@article{86987cdc-6e7a-42f1-9da8-980185b34956,
abstract = {{The purpose of this study was to explore neuroretinal transplantation in a large animal model of severe retinitis pigmentosa and to establish graft development, long-term survival, graft-host integration, and effects on the host retina. Methods Rhodopsin transgenic pigs, aged 6 months, received in one eye a fetal full-thickness neuroretinal sheet in the subretinal space by means of vitrectomy and retinotomy. Six months postoperatively, eyes were studied in the light microscope and with immunohistochemical markers. Full-field electroretinography (ERG) was performed at 4 and 6 months. Results Laminated grafts with well-organized photoreceptors, rod bipolar cells, and Muller cells were found in five of six eyes. Neuronal connections between graft and host retina were not seen. In the five eyes containing a graft, the number of surviving rods in the host retina was significantly higher compared with unoperated eyes. The ERG did not reveal any significant difference in b-wave amplitude between operated and control eyes, but the cone-derived response in operated eyes increased significantly from 4 to 6 months while the rod response in control eyes decreased significantly. Conclusions Fetal full-thickness neuroretina can be transplanted safely to an eye with severe retinal degeneration. In their major part, the transplants develop a normal laminated morphology and survive for at least 6 months. Graft and host retinal neurons do not form connections. Retinal function in the host is reduced initially by the surgical trauma, but the presence of a well-laminated graft counteracts this effect and rescues rods from degeneration.}},
author = {{Ghosh, Fredrik and Engelsberg, Karl and English, Robert and Petters, Robert}},
issn = {{1435-702X}},
keywords = {{immune privilege; retinal degeneration; photoreceptor morphology; vitreoretinal surgery}},
language = {{eng}},
number = {{6}},
pages = {{835--846}},
publisher = {{Springer}},
series = {{Graefe's Archive for Clinical and Experimental Ophthalmology}},
title = {{Long-term neuroretinal full-thickness transplants in a large animal model of severe retinitis pigmentosa.}},
url = {{https://lup.lub.lu.se/search/files/4385435/625766.pdf}},
doi = {{10.1007/s00417-006-0437-9}},
volume = {{245}},
year = {{2007}},
}