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Application of antisense transformation of a barley chitinase in studies of arbuscule formation by a mycorrhizal fungus

Troedsson, U; Olsson, Pål Axel LU and Jarl-Sunesson, Carin LU (2005) In Hereditas 142(1). p.65-72
Abstract
Barley (Hordeum vulgare L.) plants of two commercial cultivars were transformed with sense and antisense constructs of a chitinase class II gene in order to develop a transformation system for this gene in barley. Transformation of embryos with the two antisense constructs resulted in ten regenerated plants, while no plants could be obtained using the sense construct. The presence of the inserted construct could be confirmed for six of the plants by PCR analysis. This system was used to study the role of class II chitinase in the regulation of mycorrhizal symbiosis. The colonization of two of the antisense transformants by the arbuscular mycorrhizal fungus Glomus intraradices was investigated microscopically and by use of signature fatty... (More)
Barley (Hordeum vulgare L.) plants of two commercial cultivars were transformed with sense and antisense constructs of a chitinase class II gene in order to develop a transformation system for this gene in barley. Transformation of embryos with the two antisense constructs resulted in ten regenerated plants, while no plants could be obtained using the sense construct. The presence of the inserted construct could be confirmed for six of the plants by PCR analysis. This system was used to study the role of class II chitinase in the regulation of mycorrhizal symbiosis. The colonization of two of the antisense transformants by the arbuscular mycorrhizal fungus Glomus intraradices was investigated microscopically and by use of signature fatty acids. The arbuscular incidence increased in transformed barley, and one transformant supported higher extraradical mycelium biomass. It is concluded that antisense transformation of barley could be a useful tool in investigations on the symbiosis between barley and mycorrhizal fungi. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Hereditas
volume
142
issue
1
pages
65 - 72
publisher
Wiley-Blackwell
external identifiers
  • pmid:16970614
  • wos:000241491100011
  • scopus:33646035828
ISSN
1601-5223
DOI
10.1111/j.1601-5223.2005.01903.x
language
English
LU publication?
yes
id
d6ac69fa-2649-4a5c-8c43-afe1ef2d0b66 (old id 163648)
date added to LUP
2007-06-29 14:55:02
date last changed
2017-01-01 04:32:46
@article{d6ac69fa-2649-4a5c-8c43-afe1ef2d0b66,
  abstract     = {Barley (Hordeum vulgare L.) plants of two commercial cultivars were transformed with sense and antisense constructs of a chitinase class II gene in order to develop a transformation system for this gene in barley. Transformation of embryos with the two antisense constructs resulted in ten regenerated plants, while no plants could be obtained using the sense construct. The presence of the inserted construct could be confirmed for six of the plants by PCR analysis. This system was used to study the role of class II chitinase in the regulation of mycorrhizal symbiosis. The colonization of two of the antisense transformants by the arbuscular mycorrhizal fungus Glomus intraradices was investigated microscopically and by use of signature fatty acids. The arbuscular incidence increased in transformed barley, and one transformant supported higher extraradical mycelium biomass. It is concluded that antisense transformation of barley could be a useful tool in investigations on the symbiosis between barley and mycorrhizal fungi.},
  author       = {Troedsson, U and Olsson, Pål Axel and Jarl-Sunesson, Carin},
  issn         = {1601-5223},
  language     = {eng},
  number       = {1},
  pages        = {65--72},
  publisher    = {Wiley-Blackwell},
  series       = {Hereditas},
  title        = {Application of antisense transformation of a barley chitinase in studies of arbuscule formation by a mycorrhizal fungus},
  url          = {http://dx.doi.org/10.1111/j.1601-5223.2005.01903.x},
  volume       = {142},
  year         = {2005},
}