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Gene expression profiling of differentiating embryonic stem cells expressing dominant negative fibroblast growth factor receptor 2.

Gustafsson, Renata LU ; Åkerlund, Mikael LU ; Hjalt, Tord LU ; Durbeej-Hjalt, Madeleine LU and Ekblom, Peter LU (2007) In Matrix Biology 26. p.197-205
Abstract
Embryonic stein (ES) cells are derived from the inner cell mass of the blastocyst and can be cultured as three-dimensional embryoid bodies (EBs) in which embryonic pregastrulation stages are faithfully mimicked. Fibroblast growth factor receptors (mainly FGFR2) are involved in the first differentiation events during early mammalian embryogenesis. It has been demonstrated that the presence of FGFR2 is a prerequisite for laminin-111 and collagen type IV synthesis and subsequently basement membrane formation in EBs. To identify genes that are influenced by FGFR signalling, we performed global gene expression profiling of differentiating EBs expressing dominant negative FGFR2 (dnFGFR2), acquiring an extensive catalogue of down- and... (More)
Embryonic stein (ES) cells are derived from the inner cell mass of the blastocyst and can be cultured as three-dimensional embryoid bodies (EBs) in which embryonic pregastrulation stages are faithfully mimicked. Fibroblast growth factor receptors (mainly FGFR2) are involved in the first differentiation events during early mammalian embryogenesis. It has been demonstrated that the presence of FGFR2 is a prerequisite for laminin-111 and collagen type IV synthesis and subsequently basement membrane formation in EBs. To identify genes that are influenced by FGFR signalling, we performed global gene expression profiling of differentiating EBs expressing dominant negative FGFR2 (dnFGFR2), acquiring an extensive catalogue of down- and up-regulated genes. We show a strong down-regulation of endodermal and basement membrane related genes, which strengthen the view that the FGFR signalling pathway is a main stimulator of basement membrane synthesis in EBs. We further present down-regulation of genes previously not linked to FGFR signalling, and in addition an active transcription of some mesodermal related genes in differentiating dnFGFR2 EBs. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ES cell differentiation, Basement membrane, Gene expression, FGFR signalling, Microarray, Embryoid bodies
in
Matrix Biology
volume
26
pages
197 - 205
publisher
Elsevier
external identifiers
  • wos:000246018500008
  • scopus:33947186824
ISSN
1569-1802
DOI
10.1016/j.matbio.2006.10.013
language
English
LU publication?
yes
id
373bd161-1776-4bb7-bc15-e3f4e510e80d (old id 163971)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17174081&dopt=Abstract
date added to LUP
2007-07-18 10:44:11
date last changed
2017-01-01 07:17:04
@article{373bd161-1776-4bb7-bc15-e3f4e510e80d,
  abstract     = {Embryonic stein (ES) cells are derived from the inner cell mass of the blastocyst and can be cultured as three-dimensional embryoid bodies (EBs) in which embryonic pregastrulation stages are faithfully mimicked. Fibroblast growth factor receptors (mainly FGFR2) are involved in the first differentiation events during early mammalian embryogenesis. It has been demonstrated that the presence of FGFR2 is a prerequisite for laminin-111 and collagen type IV synthesis and subsequently basement membrane formation in EBs. To identify genes that are influenced by FGFR signalling, we performed global gene expression profiling of differentiating EBs expressing dominant negative FGFR2 (dnFGFR2), acquiring an extensive catalogue of down- and up-regulated genes. We show a strong down-regulation of endodermal and basement membrane related genes, which strengthen the view that the FGFR signalling pathway is a main stimulator of basement membrane synthesis in EBs. We further present down-regulation of genes previously not linked to FGFR signalling, and in addition an active transcription of some mesodermal related genes in differentiating dnFGFR2 EBs.},
  author       = {Gustafsson, Renata and Åkerlund, Mikael and Hjalt, Tord and Durbeej-Hjalt, Madeleine and Ekblom, Peter},
  issn         = {1569-1802},
  keyword      = {ES cell differentiation,Basement membrane,Gene expression,FGFR signalling,Microarray,Embryoid bodies},
  language     = {eng},
  pages        = {197--205},
  publisher    = {Elsevier},
  series       = {Matrix Biology},
  title        = {Gene expression profiling of differentiating embryonic stem cells expressing dominant negative fibroblast growth factor receptor 2.},
  url          = {http://dx.doi.org/10.1016/j.matbio.2006.10.013},
  volume       = {26},
  year         = {2007},
}