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Loss- and gain-of-function analyses reveal targets of Pax6 in the developing mouse telencephalon.

Holm, Pontus LU ; Mader, Michael T; Haubst, Nicole; Wizenmann, Andrea; Sigvardsson, Mikael LU and Gotz, Magdalena (2007) In Molecular and Cellular Neuroscience 34(1). p.99-119
Abstract
Appropriate neurogenesis and patterning of the forebrain requires the transcription factor Pax6, yet it is largely unknown how Pax6 exerts its effects at the molecular level. To characterize Pax6-mediated regulation of gene expression during murine forebrain neurogenesis, we performed microarray analysis with tissue from the dorsal Pax6-dependent telencephalon and the ventral Pax6-negative telencephalon at the onset of neurogenesis (E12) and at mid-neurogenesis (E15) in wild-type and Pax6-deficient mutant littermates. In the Pax6-deficient cortex the expression levels of various transcription factors involved in neurogenesis (like Satb2, Nfia, AP-2 gamma, NeuroD6, Ngn2, Tbr2, Bhlhb5) and the retinoic acid signalling molecule Rlbp1 were... (More)
Appropriate neurogenesis and patterning of the forebrain requires the transcription factor Pax6, yet it is largely unknown how Pax6 exerts its effects at the molecular level. To characterize Pax6-mediated regulation of gene expression during murine forebrain neurogenesis, we performed microarray analysis with tissue from the dorsal Pax6-dependent telencephalon and the ventral Pax6-negative telencephalon at the onset of neurogenesis (E12) and at mid-neurogenesis (E15) in wild-type and Pax6-deficient mutant littermates. In the Pax6-deficient cortex the expression levels of various transcription factors involved in neurogenesis (like Satb2, Nfia, AP-2 gamma, NeuroD6, Ngn2, Tbr2, Bhlhb5) and the retinoic acid signalling molecule Rlbp1 were reduced. Regulation by Pax6 could be confirmed upon electroporation of a Pax6- and a dominant-negative Pax6-containing vector into embryonic cortex. Taken together, our data reveal novel insights into the molecular pathways regulated by Pax6 during cortical neurogenesis. Most intriguingly, this analysis revealed time- and region-specific differences in Pax6-mediated transcription, explaining the specific function of Pax6 at early and later stages of neurogenesis. (c) 2006 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
neurogenesis, retinoic acid signalling, electroporation, cortex, Pax6, microarray, telencephalon, ganglionic eminence, small eye
in
Molecular and Cellular Neuroscience
volume
34
issue
1
pages
99 - 119
publisher
Elsevier
external identifiers
  • wos:000243598200011
  • scopus:33845915509
ISSN
1044-7431
DOI
10.1016/j.mcn.2006.10.008
language
English
LU publication?
yes
id
37e92264-ede4-4188-8f16-5d0cb96435b8 (old id 164122)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17158062&dopt=Abstract
date added to LUP
2007-06-29 10:44:40
date last changed
2017-06-25 03:35:41
@article{37e92264-ede4-4188-8f16-5d0cb96435b8,
  abstract     = {Appropriate neurogenesis and patterning of the forebrain requires the transcription factor Pax6, yet it is largely unknown how Pax6 exerts its effects at the molecular level. To characterize Pax6-mediated regulation of gene expression during murine forebrain neurogenesis, we performed microarray analysis with tissue from the dorsal Pax6-dependent telencephalon and the ventral Pax6-negative telencephalon at the onset of neurogenesis (E12) and at mid-neurogenesis (E15) in wild-type and Pax6-deficient mutant littermates. In the Pax6-deficient cortex the expression levels of various transcription factors involved in neurogenesis (like Satb2, Nfia, AP-2 gamma, NeuroD6, Ngn2, Tbr2, Bhlhb5) and the retinoic acid signalling molecule Rlbp1 were reduced. Regulation by Pax6 could be confirmed upon electroporation of a Pax6- and a dominant-negative Pax6-containing vector into embryonic cortex. Taken together, our data reveal novel insights into the molecular pathways regulated by Pax6 during cortical neurogenesis. Most intriguingly, this analysis revealed time- and region-specific differences in Pax6-mediated transcription, explaining the specific function of Pax6 at early and later stages of neurogenesis. (c) 2006 Elsevier Inc. All rights reserved.},
  author       = {Holm, Pontus and Mader, Michael T and Haubst, Nicole and Wizenmann, Andrea and Sigvardsson, Mikael and Gotz, Magdalena},
  issn         = {1044-7431},
  keyword      = {neurogenesis,retinoic acid signalling,electroporation,cortex,Pax6,microarray,telencephalon,ganglionic eminence,small eye},
  language     = {eng},
  number       = {1},
  pages        = {99--119},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Neuroscience},
  title        = {Loss- and gain-of-function analyses reveal targets of Pax6 in the developing mouse telencephalon.},
  url          = {http://dx.doi.org/10.1016/j.mcn.2006.10.008},
  volume       = {34},
  year         = {2007},
}