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In vitro characterization of a human neural progenitor cell coexpressing SSEA4 and CD133.

Barraud, Perrine LU ; Stott, Simon LU ; Mollgard, Kjeld; Parmar, Malin LU and Björklund, Anders LU (2007) In Journal of Neuroscience Research 85. p.250-259
Abstract
The stage-specific embryonic antigen 4 (SSEA4) is commonly used as a cell surface marker to identify the pluripotent human embryonic stem (ES) cells. Immunohistochemistry on human embryonic central nervous system revealed that SSEA4 is detectable in the early neuroepithelium, and its expression decreases as development proceeds. Flow cytometry analysis of forebrain-derived cells demonstrated that the SSEA4-expressing cells are enriched in the neural stem/progenitor cell fraction (CD133+), but are rarely codetected with the neural stem cell (NSC) marker CD15. Using a sphere-forming assay, we showed that both subfractions CD133+/SSEA4+ and CD133+/CD15+ isolated from the embryonic forebrain are enriched in neurosphere-initiating cells. In... (More)
The stage-specific embryonic antigen 4 (SSEA4) is commonly used as a cell surface marker to identify the pluripotent human embryonic stem (ES) cells. Immunohistochemistry on human embryonic central nervous system revealed that SSEA4 is detectable in the early neuroepithelium, and its expression decreases as development proceeds. Flow cytometry analysis of forebrain-derived cells demonstrated that the SSEA4-expressing cells are enriched in the neural stem/progenitor cell fraction (CD133+), but are rarely codetected with the neural stem cell (NSC) marker CD15. Using a sphere-forming assay, we showed that both subfractions CD133+/SSEA4+ and CD133+/CD15+ isolated from the embryonic forebrain are enriched in neurosphere-initiating cells. In addition CD133, SSEA4, and CD15 expression is sustained in the expanded neurosphere cells and also mark subfractions of neurosphere-initiating cells. Therefore, we propose that SSEA4 associated with CD133 can be used for both the positive selection and the enrichment of neural stem/progenitor cells from human embryonic forebrain. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
forebrain, flow cytometry, neurosphere, sphere-forming assay
in
Journal of Neuroscience Research
volume
85
pages
250 - 259
publisher
John Wiley & Sons
external identifiers
  • wos:000244058700004
  • scopus:33846853277
ISSN
1097-4547
DOI
10.1002/jnr.21116
language
English
LU publication?
yes
id
eb724f48-f1e4-46d8-a9fa-3174396f6a31 (old id 164320)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17131412&dopt=Abstract
date added to LUP
2007-07-10 10:43:25
date last changed
2017-06-25 04:25:01
@article{eb724f48-f1e4-46d8-a9fa-3174396f6a31,
  abstract     = {The stage-specific embryonic antigen 4 (SSEA4) is commonly used as a cell surface marker to identify the pluripotent human embryonic stem (ES) cells. Immunohistochemistry on human embryonic central nervous system revealed that SSEA4 is detectable in the early neuroepithelium, and its expression decreases as development proceeds. Flow cytometry analysis of forebrain-derived cells demonstrated that the SSEA4-expressing cells are enriched in the neural stem/progenitor cell fraction (CD133+), but are rarely codetected with the neural stem cell (NSC) marker CD15. Using a sphere-forming assay, we showed that both subfractions CD133+/SSEA4+ and CD133+/CD15+ isolated from the embryonic forebrain are enriched in neurosphere-initiating cells. In addition CD133, SSEA4, and CD15 expression is sustained in the expanded neurosphere cells and also mark subfractions of neurosphere-initiating cells. Therefore, we propose that SSEA4 associated with CD133 can be used for both the positive selection and the enrichment of neural stem/progenitor cells from human embryonic forebrain.},
  author       = {Barraud, Perrine and Stott, Simon and Mollgard, Kjeld and Parmar, Malin and Björklund, Anders},
  issn         = {1097-4547},
  keyword      = {forebrain,flow cytometry,neurosphere,sphere-forming assay},
  language     = {eng},
  pages        = {250--259},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Neuroscience Research},
  title        = {In vitro characterization of a human neural progenitor cell coexpressing SSEA4 and CD133.},
  url          = {http://dx.doi.org/10.1002/jnr.21116},
  volume       = {85},
  year         = {2007},
}