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Occurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue.

Bruzelius, Katharina LU ; Hoac, Tien LU ; Sundler, Roger LU ; Önning, Gunilla LU and Åkesson, Björn LU (2007) In Journal of Dairy Science 90(2). p.918-927
Abstract
To elucidate the possible role of selenoproteins for milk formation and mammary gland physiology, the activities of selenoprotein enzymes and the expression of selenoprotein genes were studied in the bovine mammary gland. Messenger RNA was demonstrated for selenoprotein P, thioredoxin reductase 1, and for glutathione peroxidase (GPx) 1, 3, and 4. Significant differences in mRNA expression between the cows were seen for GPx 1 and GPx 3. The enzyme activity of glutathione peroxidase varied approximately 16-fold among cows, and the activity of thioredoxin reductase and the concentration of soluble Se varied approximately 6-fold among cows. There were positive correlations between glutathione peroxidase activity, thioredoxin reductase... (More)
To elucidate the possible role of selenoproteins for milk formation and mammary gland physiology, the activities of selenoprotein enzymes and the expression of selenoprotein genes were studied in the bovine mammary gland. Messenger RNA was demonstrated for selenoprotein P, thioredoxin reductase 1, and for glutathione peroxidase (GPx) 1, 3, and 4. Significant differences in mRNA expression between the cows were seen for GPx 1 and GPx 3. The enzyme activity of glutathione peroxidase varied approximately 16-fold among cows, and the activity of thioredoxin reductase and the concentration of soluble Se varied approximately 6-fold among cows. There were positive correlations between glutathione peroxidase activity, thioredoxin reductase activity, and soluble Se, the correlation between glutathione peroxidase activity and soluble Se being the strongest. Furthermore, selenoprotein P expression correlated with GPx 1 mRNA expression and with soluble Se. There was also a correlation between glutathione peroxidase activity and the mRNA expression of GPx 1. The general conclusion from the data was that the activity of glutathione peroxidase and thioredoxin reductase and the mRNA expression of selenoprotein P and GPx 1 and 3 were influenced by Se status, but the expression of GPx 4 and thioredoxin reductase 1 were not. These results indicate that the Se status in mammary tissue is an important regulator of selenoprotein activity and expression, but that other factors are also in operation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glutathione peroxidase, selenoprotein, mammary tissue, thioredoxin reductase
in
Journal of Dairy Science
volume
90
issue
2
pages
918 - 927
publisher
American Dairy Science Association
external identifiers
  • wos:000243599200041
  • scopus:34247866462
ISSN
1525-3198
language
English
LU publication?
yes
id
80bb845e-f1b6-44b1-a638-9f29bd748f27 (old id 164770)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17235168&dopt=Abstract
http://jds.fass.org/cgi/content/abstract/90/2/918
date added to LUP
2007-07-11 09:10:40
date last changed
2017-11-05 03:40:38
@article{80bb845e-f1b6-44b1-a638-9f29bd748f27,
  abstract     = {To elucidate the possible role of selenoproteins for milk formation and mammary gland physiology, the activities of selenoprotein enzymes and the expression of selenoprotein genes were studied in the bovine mammary gland. Messenger RNA was demonstrated for selenoprotein P, thioredoxin reductase 1, and for glutathione peroxidase (GPx) 1, 3, and 4. Significant differences in mRNA expression between the cows were seen for GPx 1 and GPx 3. The enzyme activity of glutathione peroxidase varied approximately 16-fold among cows, and the activity of thioredoxin reductase and the concentration of soluble Se varied approximately 6-fold among cows. There were positive correlations between glutathione peroxidase activity, thioredoxin reductase activity, and soluble Se, the correlation between glutathione peroxidase activity and soluble Se being the strongest. Furthermore, selenoprotein P expression correlated with GPx 1 mRNA expression and with soluble Se. There was also a correlation between glutathione peroxidase activity and the mRNA expression of GPx 1. The general conclusion from the data was that the activity of glutathione peroxidase and thioredoxin reductase and the mRNA expression of selenoprotein P and GPx 1 and 3 were influenced by Se status, but the expression of GPx 4 and thioredoxin reductase 1 were not. These results indicate that the Se status in mammary tissue is an important regulator of selenoprotein activity and expression, but that other factors are also in operation.},
  author       = {Bruzelius, Katharina and Hoac, Tien and Sundler, Roger and Önning, Gunilla and Åkesson, Björn},
  issn         = {1525-3198},
  keyword      = {glutathione peroxidase,selenoprotein,mammary tissue,thioredoxin reductase},
  language     = {eng},
  number       = {2},
  pages        = {918--927},
  publisher    = {American Dairy Science Association},
  series       = {Journal of Dairy Science},
  title        = {Occurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue.},
  volume       = {90},
  year         = {2007},
}