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Improved xylose and arabinose utilization by an industrial recombinant Saccharomyces cerevisiae strain using evolutionary engineering

Garcia Sanchez, Rosa LU ; Karhumaa, Kaisa LU ; Fonseca, Cesar LU ; Sanchez Nogue, Violeta LU ; Almeida, Joao LU ; Larsson, Christer LU ; Bengtsson, Oskar LU ; Bettiga, Maurizio LU ; Hahn-Hägerdal, Bärbel LU and Gorwa-Grauslund, Marie-Francoise LU (2010) In Biotechnology for Biofuels 3.
Abstract
BACKGROUND: Cost-effective fermentation of lignocellulosic hydrolysate to ethanol by Saccharomyces cerevisiae requires efficient mixed sugar utilization. Notably, the rate and yield of xylose and arabinose co-fermentation to ethanol must be enhanced. RESULTS: Evolutionary engineering was used to improve the simultaneous conversion of xylose and arabinose to ethanol in a recombinant industrial Saccharomyces cerevisiae strain carrying the heterologous genes for xylose and arabinose utilization pathways integrated in the genome. The evolved strain TMB3130 displayed an increased consumption rate of xylose and arabinose under aerobic and anaerobic conditions. Improved anaerobic ethanol production was achieved at the expense of xylitol and... (More)
BACKGROUND: Cost-effective fermentation of lignocellulosic hydrolysate to ethanol by Saccharomyces cerevisiae requires efficient mixed sugar utilization. Notably, the rate and yield of xylose and arabinose co-fermentation to ethanol must be enhanced. RESULTS: Evolutionary engineering was used to improve the simultaneous conversion of xylose and arabinose to ethanol in a recombinant industrial Saccharomyces cerevisiae strain carrying the heterologous genes for xylose and arabinose utilization pathways integrated in the genome. The evolved strain TMB3130 displayed an increased consumption rate of xylose and arabinose under aerobic and anaerobic conditions. Improved anaerobic ethanol production was achieved at the expense of xylitol and glycerol but arabinose was almost stoichiometrically converted to arabitol. Further characterization of the strain indicated that the selection pressure during prolonged continuous culture in xylose and arabinose medium resulted in the improved transport of xylose and arabinose as well as increased levels of the enzymes from the introduced fungal xylose pathway. No mutation was found in any of the genes from the pentose converting pathways. CONCLUSION: To the best of our knowledge, this is the first report that characterizes the molecular mechanisms for improved mixed-pentose utilization obtained by evolutionary engineering of a recombinant S. cerevisiae strain. Increased transport of pentoses and increased activities of xylose converting enzymes contributed to the improved phenotype. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
industrial recombinant strains, mixed-sugar utilization, transport of pentoses, Saccharomyces cerevisiae, arabinose, xylose, evolutionary engineering, ethanol
in
Biotechnology for Biofuels
volume
3
publisher
BioMed Central
external identifiers
  • wos:000280325900001
ISSN
1754-6834
DOI
10.1186/1754-6834-3-13
language
English
LU publication?
yes
id
84928af8-f40b-4fc0-8424-54a14f24a7d8 (old id 1650891)
alternative location
http://www.biotechnologyforbiofuels.com/content/pdf/1754-6834-3-13.pdf
date added to LUP
2010-08-18 10:53:23
date last changed
2018-05-29 10:58:15
@article{84928af8-f40b-4fc0-8424-54a14f24a7d8,
  abstract     = {BACKGROUND: Cost-effective fermentation of lignocellulosic hydrolysate to ethanol by Saccharomyces cerevisiae requires efficient mixed sugar utilization. Notably, the rate and yield of xylose and arabinose co-fermentation to ethanol must be enhanced. RESULTS: Evolutionary engineering was used to improve the simultaneous conversion of xylose and arabinose to ethanol in a recombinant industrial Saccharomyces cerevisiae strain carrying the heterologous genes for xylose and arabinose utilization pathways integrated in the genome. The evolved strain TMB3130 displayed an increased consumption rate of xylose and arabinose under aerobic and anaerobic conditions. Improved anaerobic ethanol production was achieved at the expense of xylitol and glycerol but arabinose was almost stoichiometrically converted to arabitol. Further characterization of the strain indicated that the selection pressure during prolonged continuous culture in xylose and arabinose medium resulted in the improved transport of xylose and arabinose as well as increased levels of the enzymes from the introduced fungal xylose pathway. No mutation was found in any of the genes from the pentose converting pathways. CONCLUSION: To the best of our knowledge, this is the first report that characterizes the molecular mechanisms for improved mixed-pentose utilization obtained by evolutionary engineering of a recombinant S. cerevisiae strain. Increased transport of pentoses and increased activities of xylose converting enzymes contributed to the improved phenotype.},
  articleno    = {13},
  author       = {Garcia Sanchez, Rosa and Karhumaa, Kaisa and Fonseca, Cesar and Sanchez Nogue, Violeta and Almeida, Joao and Larsson, Christer and Bengtsson, Oskar and Bettiga, Maurizio and Hahn-Hägerdal, Bärbel and Gorwa-Grauslund, Marie-Francoise},
  issn         = {1754-6834},
  keyword      = {industrial recombinant strains,mixed-sugar utilization,transport of pentoses,Saccharomyces cerevisiae,arabinose,xylose,evolutionary engineering,ethanol},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {Biotechnology for Biofuels},
  title        = {Improved xylose and arabinose utilization by an industrial recombinant Saccharomyces cerevisiae strain using evolutionary engineering},
  url          = {http://dx.doi.org/10.1186/1754-6834-3-13},
  volume       = {3},
  year         = {2010},
}