Advanced

Interactions between Cationic Lipid Bilayers and Model Chromatin

Lundberg, Dan LU ; Berezhnoy, Nikolay V.; Lu, Chenning; Korolev, Nikolay; Su, Chun-Jen; Alfredsson, Viveka LU ; Miguel, Maria da Graca; Lindman, Björn LU and Nordenskiold, Lars (2010) In Langmuir 26(15). p.12488-12492
Abstract
Complexes formed in mixtures of cationic liposomes of varying charge density and nucleosome core particles (NCPs) or nucleosome arrays have been characterized. Under most of the conditions studied, the lipids and NCPs or arrays formed lamellar structures similar to those obtained with the liposomes and pure DNA. Thus, the dissociation of DNA from the NCP or nucleosome array and the formation of a DNA-lipid complex is thermodynamically favored, which can likely be ascribed mainly to the gain in entropy on release of the small counterions. Only at very low liposome charge densities are there indications that the NCPs/arrays do not dissociate upon interaction with the lipid bilayers. The reported results can serve as a valuable reference... (More)
Complexes formed in mixtures of cationic liposomes of varying charge density and nucleosome core particles (NCPs) or nucleosome arrays have been characterized. Under most of the conditions studied, the lipids and NCPs or arrays formed lamellar structures similar to those obtained with the liposomes and pure DNA. Thus, the dissociation of DNA from the NCP or nucleosome array and the formation of a DNA-lipid complex is thermodynamically favored, which can likely be ascribed mainly to the gain in entropy on release of the small counterions. Only at very low liposome charge densities are there indications that the NCPs/arrays do not dissociate upon interaction with the lipid bilayers. The reported results can serve as a valuable reference point in investigations of biologically more relevant systems. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
26
issue
15
pages
12488 - 12492
publisher
The American Chemical Society
external identifiers
  • wos:000280353800004
  • scopus:77955021450
ISSN
0743-7463
DOI
10.1021/la1014658
language
English
LU publication?
yes
id
f77f5411-fc1a-4fee-b21a-80f79df3ac88 (old id 1654314)
date added to LUP
2010-08-26 11:42:45
date last changed
2018-05-29 11:47:20
@article{f77f5411-fc1a-4fee-b21a-80f79df3ac88,
  abstract     = {Complexes formed in mixtures of cationic liposomes of varying charge density and nucleosome core particles (NCPs) or nucleosome arrays have been characterized. Under most of the conditions studied, the lipids and NCPs or arrays formed lamellar structures similar to those obtained with the liposomes and pure DNA. Thus, the dissociation of DNA from the NCP or nucleosome array and the formation of a DNA-lipid complex is thermodynamically favored, which can likely be ascribed mainly to the gain in entropy on release of the small counterions. Only at very low liposome charge densities are there indications that the NCPs/arrays do not dissociate upon interaction with the lipid bilayers. The reported results can serve as a valuable reference point in investigations of biologically more relevant systems.},
  author       = {Lundberg, Dan and Berezhnoy, Nikolay V. and Lu, Chenning and Korolev, Nikolay and Su, Chun-Jen and Alfredsson, Viveka and Miguel, Maria da Graca and Lindman, Björn and Nordenskiold, Lars},
  issn         = {0743-7463},
  language     = {eng},
  number       = {15},
  pages        = {12488--12492},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Interactions between Cationic Lipid Bilayers and Model Chromatin},
  url          = {http://dx.doi.org/10.1021/la1014658},
  volume       = {26},
  year         = {2010},
}