Advanced

Survival and neurotransmitter plasticity in cultured rat colonic myenteric neurons.

Sand, Elin LU ; Themner-Persson, Anna LU and Ekblad, Eva LU (2007) In Regulatory Peptides 140(3). p.109-116
Abstract
The enteric nervous system is of great importance for maintenance and proper function of the gastrointestinal tract. The aim of this study was to quantify myenteric neuronal subpopulations expressing calcitonin gene-related peptide (CGRP), galanin, neuropeptide Y (NPY), somatostatin, vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) in rat colon in vivo and after culturing. Further we investigated if culturing in the presence of CGRP, galanin, VIP, S-nitroso-N-acetyl-d,l-penicillamine (SNAP, a NO donor) or N-nitro-l-arginine methyl ester (l-NAME, a NOS inhibitor) affect neuronal survival.



After 4 days of culturing the proportions of neurons expressing CGRP, NPY, somatostatin or VIP increased as compared... (More)
The enteric nervous system is of great importance for maintenance and proper function of the gastrointestinal tract. The aim of this study was to quantify myenteric neuronal subpopulations expressing calcitonin gene-related peptide (CGRP), galanin, neuropeptide Y (NPY), somatostatin, vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) in rat colon in vivo and after culturing. Further we investigated if culturing in the presence of CGRP, galanin, VIP, S-nitroso-N-acetyl-d,l-penicillamine (SNAP, a NO donor) or N-nitro-l-arginine methyl ester (l-NAME, a NOS inhibitor) affect neuronal survival.



After 4 days of culturing the proportions of neurons expressing CGRP, NPY, somatostatin or VIP increased as compared to in vivo, while the proportions of neurons expressing galanin or NOS did not change. Neuronal survival was unaffected after culturing in media enriched with CGRP, galanin, VIP, SNAP or l-NAME. Neither did addition of CGRP, galanin nor VIP to the cultures affect the relative numbers of neurons expressing CGRP, galanin or VIP respectively. Addition of SNAP or l-NAME did not change the percentage of neurons expressing NOS.



In conclusion, cultured rat colonic myenteric neurons increase their expression of CGRP, NPY, somatostatin and VIP, suggesting that these neuropeptides are of importance for neuronal survival. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Myenteric neurons, Neuronal survival, CGRP, Galanin, NPY, Somatostatin, VIP, NOS
in
Regulatory Peptides
volume
140
issue
3
pages
109 - 116
publisher
Elsevier
external identifiers
  • wos:000246086400002
  • scopus:33947223065
ISSN
1873-1686
DOI
10.1016/j.regpep.2006.11.024
language
English
LU publication?
yes
id
bf4784c5-0765-4107-b4c4-3de2298fa363 (old id 165472)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17320199&dopt=Abstract
date added to LUP
2007-07-16 13:16:07
date last changed
2017-01-01 04:35:38
@article{bf4784c5-0765-4107-b4c4-3de2298fa363,
  abstract     = {The enteric nervous system is of great importance for maintenance and proper function of the gastrointestinal tract. The aim of this study was to quantify myenteric neuronal subpopulations expressing calcitonin gene-related peptide (CGRP), galanin, neuropeptide Y (NPY), somatostatin, vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) in rat colon in vivo and after culturing. Further we investigated if culturing in the presence of CGRP, galanin, VIP, S-nitroso-N-acetyl-d,l-penicillamine (SNAP, a NO donor) or N-nitro-l-arginine methyl ester (l-NAME, a NOS inhibitor) affect neuronal survival.<br/><br>
<br/><br>
After 4 days of culturing the proportions of neurons expressing CGRP, NPY, somatostatin or VIP increased as compared to in vivo, while the proportions of neurons expressing galanin or NOS did not change. Neuronal survival was unaffected after culturing in media enriched with CGRP, galanin, VIP, SNAP or l-NAME. Neither did addition of CGRP, galanin nor VIP to the cultures affect the relative numbers of neurons expressing CGRP, galanin or VIP respectively. Addition of SNAP or l-NAME did not change the percentage of neurons expressing NOS.<br/><br>
<br/><br>
In conclusion, cultured rat colonic myenteric neurons increase their expression of CGRP, NPY, somatostatin and VIP, suggesting that these neuropeptides are of importance for neuronal survival.},
  author       = {Sand, Elin and Themner-Persson, Anna and Ekblad, Eva},
  issn         = {1873-1686},
  keyword      = {Myenteric neurons,Neuronal survival,CGRP,Galanin,NPY,Somatostatin,VIP,NOS},
  language     = {eng},
  number       = {3},
  pages        = {109--116},
  publisher    = {Elsevier},
  series       = {Regulatory Peptides},
  title        = {Survival and neurotransmitter plasticity in cultured rat colonic myenteric neurons.},
  url          = {http://dx.doi.org/10.1016/j.regpep.2006.11.024},
  volume       = {140},
  year         = {2007},
}