Survival and neurotransmitter plasticity in cultured rat colonic myenteric neurons.
(2007) In Regulatory Peptides 140(3). p.109-116- Abstract
- The enteric nervous system is of great importance for maintenance and proper function of the gastrointestinal tract. The aim of this study was to quantify myenteric neuronal subpopulations expressing calcitonin gene-related peptide (CGRP), galanin, neuropeptide Y (NPY), somatostatin, vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) in rat colon in vivo and after culturing. Further we investigated if culturing in the presence of CGRP, galanin, VIP, S-nitroso-N-acetyl-d,l-penicillamine (SNAP, a NO donor) or N-nitro-l-arginine methyl ester (l-NAME, a NOS inhibitor) affect neuronal survival.
After 4 days of culturing the proportions of neurons expressing CGRP, NPY, somatostatin or VIP increased as compared... (More) - The enteric nervous system is of great importance for maintenance and proper function of the gastrointestinal tract. The aim of this study was to quantify myenteric neuronal subpopulations expressing calcitonin gene-related peptide (CGRP), galanin, neuropeptide Y (NPY), somatostatin, vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) in rat colon in vivo and after culturing. Further we investigated if culturing in the presence of CGRP, galanin, VIP, S-nitroso-N-acetyl-d,l-penicillamine (SNAP, a NO donor) or N-nitro-l-arginine methyl ester (l-NAME, a NOS inhibitor) affect neuronal survival.
After 4 days of culturing the proportions of neurons expressing CGRP, NPY, somatostatin or VIP increased as compared to in vivo, while the proportions of neurons expressing galanin or NOS did not change. Neuronal survival was unaffected after culturing in media enriched with CGRP, galanin, VIP, SNAP or l-NAME. Neither did addition of CGRP, galanin nor VIP to the cultures affect the relative numbers of neurons expressing CGRP, galanin or VIP respectively. Addition of SNAP or l-NAME did not change the percentage of neurons expressing NOS.
In conclusion, cultured rat colonic myenteric neurons increase their expression of CGRP, NPY, somatostatin and VIP, suggesting that these neuropeptides are of importance for neuronal survival. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/165472
- author
- Sand, Elin LU ; Themner-Persson, Anna LU and Ekblad, Eva LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Myenteric neurons, Neuronal survival, CGRP, Galanin, NPY, Somatostatin, VIP, NOS
- in
- Regulatory Peptides
- volume
- 140
- issue
- 3
- pages
- 109 - 116
- publisher
- Elsevier
- external identifiers
-
- wos:000246086400002
- scopus:33947223065
- ISSN
- 1873-1686
- DOI
- 10.1016/j.regpep.2006.11.024
- language
- English
- LU publication?
- yes
- id
- bf4784c5-0765-4107-b4c4-3de2298fa363 (old id 165472)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17320199&dopt=Abstract
- date added to LUP
- 2016-04-01 11:50:31
- date last changed
- 2022-02-25 22:08:06
@article{bf4784c5-0765-4107-b4c4-3de2298fa363,
abstract = {{The enteric nervous system is of great importance for maintenance and proper function of the gastrointestinal tract. The aim of this study was to quantify myenteric neuronal subpopulations expressing calcitonin gene-related peptide (CGRP), galanin, neuropeptide Y (NPY), somatostatin, vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) in rat colon in vivo and after culturing. Further we investigated if culturing in the presence of CGRP, galanin, VIP, S-nitroso-N-acetyl-d,l-penicillamine (SNAP, a NO donor) or N-nitro-l-arginine methyl ester (l-NAME, a NOS inhibitor) affect neuronal survival.<br/><br>
<br/><br>
After 4 days of culturing the proportions of neurons expressing CGRP, NPY, somatostatin or VIP increased as compared to in vivo, while the proportions of neurons expressing galanin or NOS did not change. Neuronal survival was unaffected after culturing in media enriched with CGRP, galanin, VIP, SNAP or l-NAME. Neither did addition of CGRP, galanin nor VIP to the cultures affect the relative numbers of neurons expressing CGRP, galanin or VIP respectively. Addition of SNAP or l-NAME did not change the percentage of neurons expressing NOS.<br/><br>
<br/><br>
In conclusion, cultured rat colonic myenteric neurons increase their expression of CGRP, NPY, somatostatin and VIP, suggesting that these neuropeptides are of importance for neuronal survival.}},
author = {{Sand, Elin and Themner-Persson, Anna and Ekblad, Eva}},
issn = {{1873-1686}},
keywords = {{Myenteric neurons; Neuronal survival; CGRP; Galanin; NPY; Somatostatin; VIP; NOS}},
language = {{eng}},
number = {{3}},
pages = {{109--116}},
publisher = {{Elsevier}},
series = {{Regulatory Peptides}},
title = {{Survival and neurotransmitter plasticity in cultured rat colonic myenteric neurons.}},
url = {{http://dx.doi.org/10.1016/j.regpep.2006.11.024}},
doi = {{10.1016/j.regpep.2006.11.024}},
volume = {{140}},
year = {{2007}},
}