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Adenoviral vectors for transient gene expression in human primitive hematopoietic cells: Applications and prospects.

Järås, Marcus LU ; Brun, Ann LU ; Karlsson, Stefan LU and Fan, Xiaolong LU (2007) In Experimental Hematology 35(3). p.343-349
Abstract
The proliferation and differentiation of primitive hematopoietic cells is tightly controlled by a number of signaling pathways. Transient blockage or enhancement of these signaling pathways may provide a new approach to manipulate the proliferation and differentiation of primitive hematopoietic cells. Adenoviral vectors have in recent years emerged as powerful tools for transient gene expression in human primitive hematopoietic cells. Important advantageous properties of adenoviral vectors include: feasible production of high-titer vector preparations, high efficiency in transducing both quiescent and actively dividing cells, high levels of transient gene expression, and a lack of mutagenic properties associated with integrating vectors.... (More)
The proliferation and differentiation of primitive hematopoietic cells is tightly controlled by a number of signaling pathways. Transient blockage or enhancement of these signaling pathways may provide a new approach to manipulate the proliferation and differentiation of primitive hematopoietic cells. Adenoviral vectors have in recent years emerged as powerful tools for transient gene expression in human primitive hematopoietic cells. Important advantageous properties of adenoviral vectors include: feasible production of high-titer vector preparations, high efficiency in transducing both quiescent and actively dividing cells, high levels of transient gene expression, and a lack of mutagenic properties associated with integrating vectors. Progress in adenoviral fiber retargeting was recently demonstrated to enable high gene transfer efficiency into nondividing human CD34(+) cells and nonobese diabetic/severe combined immunodeficient mouse bone marrow repopulating cells (SRCs), via the ubiquitously expressed CD46 as a cellular receptor. Importantly, fiber-retargeted adenoviral vectors can be engineered to report gene expression in single living CD34(+) cells, thereby facilitating the isolation and characterization of SRCs and its downstream progenitors based on intrinsic signaling pathways. This review focuses on the current progress and the potential future applications of adenoviral gene transfer into human primitive hematopoietic cells and leukemic cells. (c) 2007 International Society for Experimental Hematology. Published by Elsevier Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Hematology
volume
35
issue
3
pages
343 - 349
publisher
Elsevier
external identifiers
  • wos:000244605200001
  • scopus:33847037957
ISSN
1873-2399
DOI
10.1016/j.exphem.2006.11.004
language
English
LU publication?
yes
id
f87ea47c-2ee6-4878-a6ed-18b274eb1a83 (old id 165575)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17309814&dopt=Abstract
date added to LUP
2007-07-07 08:41:04
date last changed
2017-10-08 03:32:09
@article{f87ea47c-2ee6-4878-a6ed-18b274eb1a83,
  abstract     = {The proliferation and differentiation of primitive hematopoietic cells is tightly controlled by a number of signaling pathways. Transient blockage or enhancement of these signaling pathways may provide a new approach to manipulate the proliferation and differentiation of primitive hematopoietic cells. Adenoviral vectors have in recent years emerged as powerful tools for transient gene expression in human primitive hematopoietic cells. Important advantageous properties of adenoviral vectors include: feasible production of high-titer vector preparations, high efficiency in transducing both quiescent and actively dividing cells, high levels of transient gene expression, and a lack of mutagenic properties associated with integrating vectors. Progress in adenoviral fiber retargeting was recently demonstrated to enable high gene transfer efficiency into nondividing human CD34(+) cells and nonobese diabetic/severe combined immunodeficient mouse bone marrow repopulating cells (SRCs), via the ubiquitously expressed CD46 as a cellular receptor. Importantly, fiber-retargeted adenoviral vectors can be engineered to report gene expression in single living CD34(+) cells, thereby facilitating the isolation and characterization of SRCs and its downstream progenitors based on intrinsic signaling pathways. This review focuses on the current progress and the potential future applications of adenoviral gene transfer into human primitive hematopoietic cells and leukemic cells. (c) 2007 International Society for Experimental Hematology. Published by Elsevier Inc.},
  author       = {Järås, Marcus and Brun, Ann and Karlsson, Stefan and Fan, Xiaolong},
  issn         = {1873-2399},
  language     = {eng},
  number       = {3},
  pages        = {343--349},
  publisher    = {Elsevier},
  series       = {Experimental Hematology},
  title        = {Adenoviral vectors for transient gene expression in human primitive hematopoietic cells: Applications and prospects.},
  url          = {http://dx.doi.org/10.1016/j.exphem.2006.11.004},
  volume       = {35},
  year         = {2007},
}