Strengths and limitations of the neurosphere culture system.
Hebsgaard, Josephine LU and Parmar, Malin LU
(2006)
In Molecular Neurobiology
34(3).
p.153-161
- Abstract
- After the initial reports of free-floating cultures of neural stem cells termed neurospheres (1,2), a wide array of studies using this promising culture system emerged. In theory, this was a near-perfect system for large-scale production of neural cells for use in cell replacement therapies and to assay for and characterize neural stem cells. More than a decade later, after rigorous scrutiny and ample experimental testing of the neurosphere culture system, it has become apparent that the culture system suffers from several disadvantages, and its usefulness is limited for several applications. Nevertheless, the bulk of high-quality research produced over the last decade has also shown that under the right circumstances and for the... (More)
- After the initial reports of free-floating cultures of neural stem cells termed neurospheres (1,2), a wide array of studies using this promising culture system emerged. In theory, this was a near-perfect system for large-scale production of neural cells for use in cell replacement therapies and to assay for and characterize neural stem cells. More than a decade later, after rigorous scrutiny and ample experimental testing of the neurosphere culture system, it has become apparent that the culture system suffers from several disadvantages, and its usefulness is limited for several applications. Nevertheless, the bulk of high-quality research produced over the last decade has also shown that under the right circumstances and for the appropriate purposes, neurospheres hold up to their initial promise. This article discusses the pros and cons of the neurosphere culture system regarding its three major applications: as an assay for neural stem cells, as a model system for neurogenesis and neural development, and for expansion of neural stem cells for transplantation purposes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/165610
- author
- Hebsgaard, Josephine
LU
and Parmar, Malin
LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- clonal analysis, transplantation, regional specification, in vitro, Neurosphere, neural stem cells, neuron, glia
- in
- Molecular Neurobiology
- volume
- 34
- issue
- 3
- pages
- 153 - 161
- publisher
- Humana Press
- external identifiers
-
- wos:000250119900001
- scopus:33847034841
- ISSN
- 1559-1182
- language
- English
- LU publication?
- yes
- id
- 95aec330-8c83-45db-8584-12ea13f30d3e (old id 165610)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17308349&dopt=Abstract
- date added to LUP
- 2016-04-01 16:00:17
- date last changed
- 2022-03-14 21:25:57
@article{95aec330-8c83-45db-8584-12ea13f30d3e,
abstract = {{After the initial reports of free-floating cultures of neural stem cells termed neurospheres (1,2), a wide array of studies using this promising culture system emerged. In theory, this was a near-perfect system for large-scale production of neural cells for use in cell replacement therapies and to assay for and characterize neural stem cells. More than a decade later, after rigorous scrutiny and ample experimental testing of the neurosphere culture system, it has become apparent that the culture system suffers from several disadvantages, and its usefulness is limited for several applications. Nevertheless, the bulk of high-quality research produced over the last decade has also shown that under the right circumstances and for the appropriate purposes, neurospheres hold up to their initial promise. This article discusses the pros and cons of the neurosphere culture system regarding its three major applications: as an assay for neural stem cells, as a model system for neurogenesis and neural development, and for expansion of neural stem cells for transplantation purposes.}},
author = {{Hebsgaard, Josephine and Parmar, Malin}},
issn = {{1559-1182}},
keywords = {{clonal analysis; transplantation; regional specification; in vitro; Neurosphere; neural stem cells; neuron; glia}},
language = {{eng}},
number = {{3}},
pages = {{153--161}},
publisher = {{Humana Press}},
series = {{Molecular Neurobiology}},
title = {{Strengths and limitations of the neurosphere culture system.}},
url = {{http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17308349&dopt=Abstract}},
volume = {{34}},
year = {{2006}},
}