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Strengths and limitations of the neurosphere culture system.

Hebsgaard, Josephine LU and Parmar, Malin LU orcid (2006) In Molecular Neurobiology 34(3). p.153-161
Abstract
After the initial reports of free-floating cultures of neural stem cells termed neurospheres (1,2), a wide array of studies using this promising culture system emerged. In theory, this was a near-perfect system for large-scale production of neural cells for use in cell replacement therapies and to assay for and characterize neural stem cells. More than a decade later, after rigorous scrutiny and ample experimental testing of the neurosphere culture system, it has become apparent that the culture system suffers from several disadvantages, and its usefulness is limited for several applications. Nevertheless, the bulk of high-quality research produced over the last decade has also shown that under the right circumstances and for the... (More)
After the initial reports of free-floating cultures of neural stem cells termed neurospheres (1,2), a wide array of studies using this promising culture system emerged. In theory, this was a near-perfect system for large-scale production of neural cells for use in cell replacement therapies and to assay for and characterize neural stem cells. More than a decade later, after rigorous scrutiny and ample experimental testing of the neurosphere culture system, it has become apparent that the culture system suffers from several disadvantages, and its usefulness is limited for several applications. Nevertheless, the bulk of high-quality research produced over the last decade has also shown that under the right circumstances and for the appropriate purposes, neurospheres hold up to their initial promise. This article discusses the pros and cons of the neurosphere culture system regarding its three major applications: as an assay for neural stem cells, as a model system for neurogenesis and neural development, and for expansion of neural stem cells for transplantation purposes. (Less)
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
clonal analysis, transplantation, regional specification, in vitro, Neurosphere, neural stem cells, neuron, glia
in
Molecular Neurobiology
volume
34
issue
3
pages
153 - 161
publisher
Humana Press
external identifiers
  • wos:000250119900001
  • scopus:33847034841
ISSN
1559-1182
language
English
LU publication?
yes
id
95aec330-8c83-45db-8584-12ea13f30d3e (old id 165610)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17308349&dopt=Abstract
date added to LUP
2016-04-01 16:00:17
date last changed
2021-10-06 04:00:51
@article{95aec330-8c83-45db-8584-12ea13f30d3e,
  abstract     = {After the initial reports of free-floating cultures of neural stem cells termed neurospheres (1,2), a wide array of studies using this promising culture system emerged. In theory, this was a near-perfect system for large-scale production of neural cells for use in cell replacement therapies and to assay for and characterize neural stem cells. More than a decade later, after rigorous scrutiny and ample experimental testing of the neurosphere culture system, it has become apparent that the culture system suffers from several disadvantages, and its usefulness is limited for several applications. Nevertheless, the bulk of high-quality research produced over the last decade has also shown that under the right circumstances and for the appropriate purposes, neurospheres hold up to their initial promise. This article discusses the pros and cons of the neurosphere culture system regarding its three major applications: as an assay for neural stem cells, as a model system for neurogenesis and neural development, and for expansion of neural stem cells for transplantation purposes.},
  author       = {Hebsgaard, Josephine and Parmar, Malin},
  issn         = {1559-1182},
  language     = {eng},
  number       = {3},
  pages        = {153--161},
  publisher    = {Humana Press},
  series       = {Molecular Neurobiology},
  title        = {Strengths and limitations of the neurosphere culture system.},
  url          = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17308349&dopt=Abstract},
  volume       = {34},
  year         = {2006},
}