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A constitutive endogenous osteopontin production is important for macrophage function and differentiation.

Nyström, Tobias LU ; Dunér, Pontus LU and Hultgårdh, Anna LU (2007) In Experimental Cell Research 313(6). p.1149-1160
Abstract
Macrophages are involved in the pathological process underlying atherosclerosis and constitutively express the multifunctional protein osteopontin which has important exogenous effects on these cells. However, the effect of the endogenous osteopontin expression on macrophage function has been sparsely studied. To shed light on the importance of the endogenous osteopontin expression, RAW 264.7 macrophage-like cells were silenced in osteopontin expression using RNAi. The cells were analysed for basic functions including attachment, migration, apoptosis and for the expression of macrophage differentiation markers and cytokines. The macrophages with silenced osteopontin expression showed impaired migration and an increased rate of serum... (More)
Macrophages are involved in the pathological process underlying atherosclerosis and constitutively express the multifunctional protein osteopontin which has important exogenous effects on these cells. However, the effect of the endogenous osteopontin expression on macrophage function has been sparsely studied. To shed light on the importance of the endogenous osteopontin expression, RAW 264.7 macrophage-like cells were silenced in osteopontin expression using RNAi. The cells were analysed for basic functions including attachment, migration, apoptosis and for the expression of macrophage differentiation markers and cytokines. The macrophages with silenced osteopontin expression showed impaired migration and an increased rate of serum starvation-induced apoptosis as compared to osteopontin-producing control cells. Furthermore, the cells with silence osteopontin expression had an altered phenotype with monocyte-like characteristics, including decreased expression of macrophage scavenger receptor A type 1. The altered phenotype of these cells could not be reversed by presence of extracellular osteopontin. In addition the cells with silenced osteopontin expression had a lower expression of IL-12 mRNA and the anti-apoptotic Flip mRNA. We conclude that a constitutive endogenous osteopontin production is important for proper basic functions of macrophages and our study indicates that the constitutive osteopontin production is involved in maintaining macrophages in a differentiated phenotype. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
osteopontin silencing, CD44, macrophages, differentiation, apoptosis, Akt, flip, activation
in
Experimental Cell Research
volume
313
issue
6
pages
1149 - 1160
publisher
Academic Press
external identifiers
  • wos:000245412300007
  • scopus:33947249865
ISSN
1090-2422
DOI
10.1016/j.yexcr.2006.12.026
language
English
LU publication?
yes
id
6cec8a2e-105d-48c0-b70b-7ceed791577c (old id 165647)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17306792&dopt=Abstract
date added to LUP
2007-07-18 13:39:17
date last changed
2017-08-20 03:34:47
@article{6cec8a2e-105d-48c0-b70b-7ceed791577c,
  abstract     = {Macrophages are involved in the pathological process underlying atherosclerosis and constitutively express the multifunctional protein osteopontin which has important exogenous effects on these cells. However, the effect of the endogenous osteopontin expression on macrophage function has been sparsely studied. To shed light on the importance of the endogenous osteopontin expression, RAW 264.7 macrophage-like cells were silenced in osteopontin expression using RNAi. The cells were analysed for basic functions including attachment, migration, apoptosis and for the expression of macrophage differentiation markers and cytokines. The macrophages with silenced osteopontin expression showed impaired migration and an increased rate of serum starvation-induced apoptosis as compared to osteopontin-producing control cells. Furthermore, the cells with silence osteopontin expression had an altered phenotype with monocyte-like characteristics, including decreased expression of macrophage scavenger receptor A type 1. The altered phenotype of these cells could not be reversed by presence of extracellular osteopontin. In addition the cells with silenced osteopontin expression had a lower expression of IL-12 mRNA and the anti-apoptotic Flip mRNA. We conclude that a constitutive endogenous osteopontin production is important for proper basic functions of macrophages and our study indicates that the constitutive osteopontin production is involved in maintaining macrophages in a differentiated phenotype.},
  author       = {Nyström, Tobias and Dunér, Pontus and Hultgårdh, Anna},
  issn         = {1090-2422},
  keyword      = {osteopontin silencing,CD44,macrophages,differentiation,apoptosis,Akt,flip,activation},
  language     = {eng},
  number       = {6},
  pages        = {1149--1160},
  publisher    = {Academic Press},
  series       = {Experimental Cell Research},
  title        = {A constitutive endogenous osteopontin production is important for macrophage function and differentiation.},
  url          = {http://dx.doi.org/10.1016/j.yexcr.2006.12.026},
  volume       = {313},
  year         = {2007},
}