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The Transcriptional Activity of Neurog3 Affects Migration and Differentiation of Ectopic Endocrine Cells in Chicken Endoderm

Rosenberg, Louise C.; Lafon, Merete L.; Karup, Jesper LU ; Yassin, Hani; Jensen, Jan Nygaard; Serup, Palle and Hecksher-Sorensen, Jacob (2010) In Developmental Dynamics 239(7). p.1950-1966
Abstract
Neurog3 is expressed transiently in pancreatic endocrine progenitors where it is responsible for activating a transcription factor cascade which eventually defines the mature endocrine cells. However, the mechanism by which Neurog3 regulates different aspects of the endocrine differentiation program is less clear. In this report we used in ovo electroporation to investigate how manipulation of Neurog3 protein activity affected migration, differentiation and fate determination. We found that changes in the onset of Neurog3 expression only had minor effect on differentiation. However increasing the transcriptional activity of Neurog3 by fusing it to VP16 or co-electroporating with Ep300 caused the electroporated cells to migrate rather than... (More)
Neurog3 is expressed transiently in pancreatic endocrine progenitors where it is responsible for activating a transcription factor cascade which eventually defines the mature endocrine cells. However, the mechanism by which Neurog3 regulates different aspects of the endocrine differentiation program is less clear. In this report we used in ovo electroporation to investigate how manipulation of Neurog3 protein activity affected migration, differentiation and fate determination. We found that changes in the onset of Neurog3 expression only had minor effect on differentiation. However increasing the transcriptional activity of Neurog3 by fusing it to VP16 or co-electroporating with Ep300 caused the electroporated cells to migrate rather than differentiate. In contrast, reducing the transcriptional activity of Neurog3 by deleting parts of the activation domain, by fusing Neurog3 to the engrailed repressor domain, or co-electroporating with Hdac1 greatly increased the proportion of glucagon expressing cells. Developmental Dynamics 239:1950-1966, 2010. (C) 2010 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
endocrine competence, glucagon, differentiation, pancreas, fluorescence, whole-mount, in ovo electroporation, neurogenin 3, Neurog3, Ngn3
in
Developmental Dynamics
volume
239
issue
7
pages
1950 - 1966
publisher
John Wiley & Sons
external identifiers
  • wos:000279663800005
  • scopus:77953738786
ISSN
1097-0177
DOI
10.1002/dvdy.22329
language
English
LU publication?
yes
id
04631992-b8d0-4811-8b1f-f0b672478306 (old id 1657988)
date added to LUP
2010-08-20 09:36:57
date last changed
2018-05-29 11:36:47
@article{04631992-b8d0-4811-8b1f-f0b672478306,
  abstract     = {Neurog3 is expressed transiently in pancreatic endocrine progenitors where it is responsible for activating a transcription factor cascade which eventually defines the mature endocrine cells. However, the mechanism by which Neurog3 regulates different aspects of the endocrine differentiation program is less clear. In this report we used in ovo electroporation to investigate how manipulation of Neurog3 protein activity affected migration, differentiation and fate determination. We found that changes in the onset of Neurog3 expression only had minor effect on differentiation. However increasing the transcriptional activity of Neurog3 by fusing it to VP16 or co-electroporating with Ep300 caused the electroporated cells to migrate rather than differentiate. In contrast, reducing the transcriptional activity of Neurog3 by deleting parts of the activation domain, by fusing Neurog3 to the engrailed repressor domain, or co-electroporating with Hdac1 greatly increased the proportion of glucagon expressing cells. Developmental Dynamics 239:1950-1966, 2010. (C) 2010 Wiley-Liss, Inc.},
  author       = {Rosenberg, Louise C. and Lafon, Merete L. and Karup, Jesper and Yassin, Hani and Jensen, Jan Nygaard and Serup, Palle and Hecksher-Sorensen, Jacob},
  issn         = {1097-0177},
  keyword      = {endocrine competence,glucagon,differentiation,pancreas,fluorescence,whole-mount,in ovo electroporation,neurogenin 3,Neurog3,Ngn3},
  language     = {eng},
  number       = {7},
  pages        = {1950--1966},
  publisher    = {John Wiley & Sons},
  series       = {Developmental Dynamics},
  title        = {The Transcriptional Activity of Neurog3 Affects Migration and Differentiation of Ectopic Endocrine Cells in Chicken Endoderm},
  url          = {http://dx.doi.org/10.1002/dvdy.22329},
  volume       = {239},
  year         = {2010},
}