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Optimizing a GC-MS method for screening of Stachybotrys mycotoxins in indoor environments.

Bloom, Erica LU ; Bal, Karol LU ; Nyman, Eva and Larsson, Lennart LU (2007) In Journal of Environmental Monitoring 9(2). p.151-156
Abstract
Presence of Stachybotrys chartarum in indoor environments has been linked to building-associated disease, however, the causative agents are unknown. Verrucarol (VER) and trichodermol (TRID) are hydrolysis products of some major S. chartarum mycotoxins, i.e. macrocyclic trichothecenes and trichodermin. We optimized gas chromatography-mass spectrometry (GC-MS) methods for detecting VER and TRID in S. chartarum-contaminated indoor environmental samples. Heptafluorobutyryl derivatives of both VER and TRID exhibited little MS fragmentation and gave much higher detection sensitivity (sub-picogram injected onto the GC column), both in GC-MS and GC-MSMS, than trimethylsilyl derivatives. Optimal detection sensitivity and speci. city was achieved by... (More)
Presence of Stachybotrys chartarum in indoor environments has been linked to building-associated disease, however, the causative agents are unknown. Verrucarol (VER) and trichodermol (TRID) are hydrolysis products of some major S. chartarum mycotoxins, i.e. macrocyclic trichothecenes and trichodermin. We optimized gas chromatography-mass spectrometry (GC-MS) methods for detecting VER and TRID in S. chartarum-contaminated indoor environmental samples. Heptafluorobutyryl derivatives of both VER and TRID exhibited little MS fragmentation and gave much higher detection sensitivity (sub-picogram injected onto the GC column), both in GC-MS and GC-MSMS, than trimethylsilyl derivatives. Optimal detection sensitivity and speci. city was achieved by combining chemical ionization and negative ion (NICI) detection with MSMS. With this method, VER and TRID were detected in building materials colonized by S. chartarum and TRID was demonstrated in dust settled in the breathing zone in a house where an inner wall was colonized. In summary, we have shown that NICI-GC-MSMS can be used to demonstrate mycotoxins in house dust in S. chartarum-contaminated dwellings. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Environmental Monitoring
volume
9
issue
2
pages
151 - 156
publisher
Royal Society of Chemistry
external identifiers
  • wos:000244008600003
  • scopus:33846786647
ISSN
1464-0325
DOI
10.1039/b613853e
language
English
LU publication?
yes
id
e89ef089-a0a7-4310-84a8-77f4e44e7b78 (old id 165843)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17285157&dopt=Abstract
date added to LUP
2007-07-09 15:32:53
date last changed
2017-05-21 03:36:43
@article{e89ef089-a0a7-4310-84a8-77f4e44e7b78,
  abstract     = {Presence of Stachybotrys chartarum in indoor environments has been linked to building-associated disease, however, the causative agents are unknown. Verrucarol (VER) and trichodermol (TRID) are hydrolysis products of some major S. chartarum mycotoxins, i.e. macrocyclic trichothecenes and trichodermin. We optimized gas chromatography-mass spectrometry (GC-MS) methods for detecting VER and TRID in S. chartarum-contaminated indoor environmental samples. Heptafluorobutyryl derivatives of both VER and TRID exhibited little MS fragmentation and gave much higher detection sensitivity (sub-picogram injected onto the GC column), both in GC-MS and GC-MSMS, than trimethylsilyl derivatives. Optimal detection sensitivity and speci. city was achieved by combining chemical ionization and negative ion (NICI) detection with MSMS. With this method, VER and TRID were detected in building materials colonized by S. chartarum and TRID was demonstrated in dust settled in the breathing zone in a house where an inner wall was colonized. In summary, we have shown that NICI-GC-MSMS can be used to demonstrate mycotoxins in house dust in S. chartarum-contaminated dwellings.},
  author       = {Bloom, Erica and Bal, Karol and Nyman, Eva and Larsson, Lennart},
  issn         = {1464-0325},
  language     = {eng},
  number       = {2},
  pages        = {151--156},
  publisher    = {Royal Society of Chemistry},
  series       = {Journal of Environmental Monitoring},
  title        = {Optimizing a GC-MS method for screening of Stachybotrys mycotoxins in indoor environments.},
  url          = {http://dx.doi.org/10.1039/b613853e},
  volume       = {9},
  year         = {2007},
}