Optimizing a GC-MS method for screening of Stachybotrys mycotoxins in indoor environments.
(2007) In Journal of Environmental Monitoring 9(2). p.151-156- Abstract
- Presence of Stachybotrys chartarum in indoor environments has been linked to building-associated disease, however, the causative agents are unknown. Verrucarol (VER) and trichodermol (TRID) are hydrolysis products of some major S. chartarum mycotoxins, i.e. macrocyclic trichothecenes and trichodermin. We optimized gas chromatography-mass spectrometry (GC-MS) methods for detecting VER and TRID in S. chartarum-contaminated indoor environmental samples. Heptafluorobutyryl derivatives of both VER and TRID exhibited little MS fragmentation and gave much higher detection sensitivity (sub-picogram injected onto the GC column), both in GC-MS and GC-MSMS, than trimethylsilyl derivatives. Optimal detection sensitivity and speci. city was achieved by... (More)
- Presence of Stachybotrys chartarum in indoor environments has been linked to building-associated disease, however, the causative agents are unknown. Verrucarol (VER) and trichodermol (TRID) are hydrolysis products of some major S. chartarum mycotoxins, i.e. macrocyclic trichothecenes and trichodermin. We optimized gas chromatography-mass spectrometry (GC-MS) methods for detecting VER and TRID in S. chartarum-contaminated indoor environmental samples. Heptafluorobutyryl derivatives of both VER and TRID exhibited little MS fragmentation and gave much higher detection sensitivity (sub-picogram injected onto the GC column), both in GC-MS and GC-MSMS, than trimethylsilyl derivatives. Optimal detection sensitivity and speci. city was achieved by combining chemical ionization and negative ion (NICI) detection with MSMS. With this method, VER and TRID were detected in building materials colonized by S. chartarum and TRID was demonstrated in dust settled in the breathing zone in a house where an inner wall was colonized. In summary, we have shown that NICI-GC-MSMS can be used to demonstrate mycotoxins in house dust in S. chartarum-contaminated dwellings. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/165843
- author
- Bloom, Erica LU ; Bal, Karol LU ; Nyman, Eva and Larsson, Lennart LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Environmental Monitoring
- volume
- 9
- issue
- 2
- pages
- 151 - 156
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000244008600003
- scopus:33846786647
- pmid:17285157
- ISSN
- 1464-0325
- DOI
- 10.1039/b613853e
- language
- English
- LU publication?
- yes
- id
- e89ef089-a0a7-4310-84a8-77f4e44e7b78 (old id 165843)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17285157&dopt=Abstract
- date added to LUP
- 2016-04-01 12:01:51
- date last changed
- 2022-01-26 21:45:16
@article{e89ef089-a0a7-4310-84a8-77f4e44e7b78, abstract = {{Presence of Stachybotrys chartarum in indoor environments has been linked to building-associated disease, however, the causative agents are unknown. Verrucarol (VER) and trichodermol (TRID) are hydrolysis products of some major S. chartarum mycotoxins, i.e. macrocyclic trichothecenes and trichodermin. We optimized gas chromatography-mass spectrometry (GC-MS) methods for detecting VER and TRID in S. chartarum-contaminated indoor environmental samples. Heptafluorobutyryl derivatives of both VER and TRID exhibited little MS fragmentation and gave much higher detection sensitivity (sub-picogram injected onto the GC column), both in GC-MS and GC-MSMS, than trimethylsilyl derivatives. Optimal detection sensitivity and speci. city was achieved by combining chemical ionization and negative ion (NICI) detection with MSMS. With this method, VER and TRID were detected in building materials colonized by S. chartarum and TRID was demonstrated in dust settled in the breathing zone in a house where an inner wall was colonized. In summary, we have shown that NICI-GC-MSMS can be used to demonstrate mycotoxins in house dust in S. chartarum-contaminated dwellings.}}, author = {{Bloom, Erica and Bal, Karol and Nyman, Eva and Larsson, Lennart}}, issn = {{1464-0325}}, language = {{eng}}, number = {{2}}, pages = {{151--156}}, publisher = {{Royal Society of Chemistry}}, series = {{Journal of Environmental Monitoring}}, title = {{Optimizing a GC-MS method for screening of Stachybotrys mycotoxins in indoor environments.}}, url = {{http://dx.doi.org/10.1039/b613853e}}, doi = {{10.1039/b613853e}}, volume = {{9}}, year = {{2007}}, }