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Forward RNAi screens in human stem cells.

Karlsson, Christine LU ; Larsson, Jonas LU and Baudet, Aurelie LU (2010) In Methods in Molecular Biology 650. p.29-43
Abstract
Identifying the genes and pathways that regulate self-renewal and differentiation in somatic stem cells is a central goal in stem cell and cancer biology. Here, we describe a method for RNAi-based screens in primary human hematopoietic stem and progenitor cells. These cells are suitable targets for complex, selection-based screens using pooled lentiviral shRNA libraries. The screening approach is a promising new tool to dissect regulatory mechanisms in hematopoietic and somatic stem cells, in general, and may be particularly useful to identify gene targets and modifiers that can be further exploited in strategies for ex vivo stem cell expansion.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
650
pages
29 - 43
publisher
Springer
external identifiers
  • pmid:20686941
  • scopus:79952026018
ISSN
1940-6029
DOI
10.1007/978-1-60761-769-3_3
language
English
LU publication?
yes
id
be582ed4-924c-486b-8bc6-68380274c65e (old id 1665561)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20686941?dopt=Abstract
date added to LUP
2010-09-02 10:59:24
date last changed
2018-05-29 11:55:38
@article{be582ed4-924c-486b-8bc6-68380274c65e,
  abstract     = {Identifying the genes and pathways that regulate self-renewal and differentiation in somatic stem cells is a central goal in stem cell and cancer biology. Here, we describe a method for RNAi-based screens in primary human hematopoietic stem and progenitor cells. These cells are suitable targets for complex, selection-based screens using pooled lentiviral shRNA libraries. The screening approach is a promising new tool to dissect regulatory mechanisms in hematopoietic and somatic stem cells, in general, and may be particularly useful to identify gene targets and modifiers that can be further exploited in strategies for ex vivo stem cell expansion.},
  author       = {Karlsson, Christine and Larsson, Jonas and Baudet, Aurelie},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {29--43},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Forward RNAi screens in human stem cells.},
  url          = {http://dx.doi.org/10.1007/978-1-60761-769-3_3},
  volume       = {650},
  year         = {2010},
}