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Molecular evidence of stereo-specific lactoferrin dimers in solution.

Persson, Björn LU ; Lund, Mikael LU ; Forsman, Jan LU ; Chatterton, Dereck E W and Åkesson, Torbjörn LU (2010) In Biophysical Chemistry 151(3). p.187-189
Abstract
Gathering experimental evidence suggests that bovine as well as human lactoferrin self-associate in aqueous solution. Still, a molecular level explanation is unavailable. Using force field based molecular modeling of the protein-protein interaction free energy we demonstrate (1) that lactoferrin forms highly stereo-specific dimers at neutral pH and (2) that the self-association is driven by a high charge complementarity across the contact surface of the proteins. Our theoretical predictions of dimer formation are verified by electrophoretic mobility and N-terminal sequence analysis on bovine lactoferrin.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biophysical Chemistry
volume
151
issue
3
pages
187 - 189
publisher
Elsevier
external identifiers
  • wos:000281920700013
  • pmid:20674143
  • scopus:77955846507
ISSN
1873-4200
DOI
10.1016/j.bpc.2010.06.005
language
English
LU publication?
yes
id
b6f094c7-4785-468a-b133-4faf7acc74df (old id 1665732)
date added to LUP
2010-09-14 09:50:19
date last changed
2018-06-17 04:07:22
@article{b6f094c7-4785-468a-b133-4faf7acc74df,
  abstract     = {Gathering experimental evidence suggests that bovine as well as human lactoferrin self-associate in aqueous solution. Still, a molecular level explanation is unavailable. Using force field based molecular modeling of the protein-protein interaction free energy we demonstrate (1) that lactoferrin forms highly stereo-specific dimers at neutral pH and (2) that the self-association is driven by a high charge complementarity across the contact surface of the proteins. Our theoretical predictions of dimer formation are verified by electrophoretic mobility and N-terminal sequence analysis on bovine lactoferrin.},
  author       = {Persson, Björn and Lund, Mikael and Forsman, Jan and Chatterton, Dereck E W and Åkesson, Torbjörn},
  issn         = {1873-4200},
  language     = {eng},
  number       = {3},
  pages        = {187--189},
  publisher    = {Elsevier},
  series       = {Biophysical Chemistry},
  title        = {Molecular evidence of stereo-specific lactoferrin dimers in solution.},
  url          = {http://dx.doi.org/10.1016/j.bpc.2010.06.005},
  volume       = {151},
  year         = {2010},
}