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Do type 1 fimbriae promote inflammation in the human urinary tract?

Bergsten, Göran LU ; Wullt, Björn LU ; Schembri, Mark A; Leijonhufvud, Irene LU and Svanborg, Catharina LU (2007) In Cellular Microbiology 9(7). p.1766-1781
Abstract
Type 1 fimbriae have been implicated as virulence factors in animal models of urinary tract infection (UTI), but the function in human disease remains unclear. This study used a human challenge model to examine if type 1 fimbriae trigger inflammation in the urinary tract. The asymptomatic bacteriuria strain Escherichia coli 83972, which fails to express type 1 fimbriae, due to a 4.25 kb fimB-fimD deletion, was reconstituted with a functional fim gene cluster and fimbrial expression was monitored through a gfp reporter. Each patient was inoculated with the fim+ or fim- variants on separate occasions, and the host response to type 1 fimbriae was quantified by intraindividual comparisons of the responses to the fim+ or fim- isogens, using... (More)
Type 1 fimbriae have been implicated as virulence factors in animal models of urinary tract infection (UTI), but the function in human disease remains unclear. This study used a human challenge model to examine if type 1 fimbriae trigger inflammation in the urinary tract. The asymptomatic bacteriuria strain Escherichia coli 83972, which fails to express type 1 fimbriae, due to a 4.25 kb fimB-fimD deletion, was reconstituted with a functional fim gene cluster and fimbrial expression was monitored through a gfp reporter. Each patient was inoculated with the fim+ or fim- variants on separate occasions, and the host response to type 1 fimbriae was quantified by intraindividual comparisons of the responses to the fim+ or fim- isogens, using cytokines and neutrophils as end-points. Type 1 fimbriae did not promote inflammation and adherence was poor, as examined on exfoliated cells in urine. This was unexpected, as type 1 fimbriae enhanced the inflammatory response to the same strain in the murine urinary tract and as P fimbrial expression by E. coli 83972 enhances adherence and inflammation in challenged patients. We conclude that type 1 fimbriae do not contribute to the mucosal inflammatory response in the human urinary tract. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cellular Microbiology
volume
9
issue
7
pages
1766 - 1781
publisher
Wiley-Blackwell
external identifiers
  • wos:000247319000014
  • scopus:34250698573
ISSN
1462-5814
DOI
10.1111/j.1462-5822.2007.00912.x
language
English
LU publication?
yes
id
b5d193d1-b728-4d60-beb3-482926d9ef55 (old id 166574)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17359236&dopt=Abstract
date added to LUP
2007-07-04 08:22:22
date last changed
2017-03-05 03:32:52
@article{b5d193d1-b728-4d60-beb3-482926d9ef55,
  abstract     = {Type 1 fimbriae have been implicated as virulence factors in animal models of urinary tract infection (UTI), but the function in human disease remains unclear. This study used a human challenge model to examine if type 1 fimbriae trigger inflammation in the urinary tract. The asymptomatic bacteriuria strain Escherichia coli 83972, which fails to express type 1 fimbriae, due to a 4.25 kb fimB-fimD deletion, was reconstituted with a functional fim gene cluster and fimbrial expression was monitored through a gfp reporter. Each patient was inoculated with the fim+ or fim- variants on separate occasions, and the host response to type 1 fimbriae was quantified by intraindividual comparisons of the responses to the fim+ or fim- isogens, using cytokines and neutrophils as end-points. Type 1 fimbriae did not promote inflammation and adherence was poor, as examined on exfoliated cells in urine. This was unexpected, as type 1 fimbriae enhanced the inflammatory response to the same strain in the murine urinary tract and as P fimbrial expression by E. coli 83972 enhances adherence and inflammation in challenged patients. We conclude that type 1 fimbriae do not contribute to the mucosal inflammatory response in the human urinary tract.},
  author       = {Bergsten, Göran and Wullt, Björn and Schembri, Mark A and Leijonhufvud, Irene and Svanborg, Catharina},
  issn         = {1462-5814},
  language     = {eng},
  number       = {7},
  pages        = {1766--1781},
  publisher    = {Wiley-Blackwell},
  series       = {Cellular Microbiology},
  title        = {Do type 1 fimbriae promote inflammation in the human urinary tract?},
  url          = {http://dx.doi.org/10.1111/j.1462-5822.2007.00912.x},
  volume       = {9},
  year         = {2007},
}