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High-Resolution Genotyping of Chlamydia trachomatis Strains by Multilocus Sequence Analysis.

Klint, Markus; Fuxelius, Hans-Henrik; Rostlinger Goldkuhl, Renée; Skarin, Hanna; Rutemark, Christian; Andersson, Siv G E; Persson, Kenneth LU and Herrmann, Bjorn (2007) In Journal of Clinical Microbiology 45(5). p.1410-1414
Abstract
Genotyping of Chlamydia trachomatis is limited by the low sequence variation in the genome, and no adequate method is available for analysis of the spread of chlamydial infections in the community. We have developed a multillocus sequence typing (MLST) system based on five target regions and compared it with analysis of ompA, the single gene most extensively used for genotyping. Sequence determination of 16 reference strains, comprising all major serotypes, serotypes A to L3, showed that the number of genetic variants in the five separate target regions ranged from 8 to 16. The genetic variation in 47 clinical C. trachomatis isolates of representative serotypes (14 serotype D, 12 serotype E, 11 serotype G, and 10 serotype K strains) was... (More)
Genotyping of Chlamydia trachomatis is limited by the low sequence variation in the genome, and no adequate method is available for analysis of the spread of chlamydial infections in the community. We have developed a multillocus sequence typing (MLST) system based on five target regions and compared it with analysis of ompA, the single gene most extensively used for genotyping. Sequence determination of 16 reference strains, comprising all major serotypes, serotypes A to L3, showed that the number of genetic variants in the five separate target regions ranged from 8 to 16. The genetic variation in 47 clinical C. trachomatis isolates of representative serotypes (14 serotype D, 12 serotype E, 11 serotype G, and 10 serotype K strains) was analyzed; and the MLST system detected 32 variants, whereas 12 variants were detected by using ompA analysis. Specimens of the predominant serotype, serotype E, were differentiated into seven genotypes by MLST but into only two by ompA analysis. The MLST system was applied to C. trachomatis specimens from a population of men who have sex with men and was able to differentiate 10 specimens of one predominant ompA genotype G variant into four distinct MLST variants. To conclude, our MLST system can be used to discriminate C. trachomatis strains and can be applied to high-resolution molecular epidemiology. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Microbiology
volume
45
issue
5
pages
1410 - 1414
publisher
American Society for Microbiology
external identifiers
  • wos:000246600300005
  • scopus:34248523973
ISSN
1098-660X
DOI
10.1128/JCM.02301-06
language
English
LU publication?
yes
id
d8c94c8b-f790-4d81-881a-b4f75d921303 (old id 166928)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17329456&dopt=Abstract
date added to LUP
2007-07-25 15:17:47
date last changed
2017-11-05 04:33:38
@article{d8c94c8b-f790-4d81-881a-b4f75d921303,
  abstract     = {Genotyping of Chlamydia trachomatis is limited by the low sequence variation in the genome, and no adequate method is available for analysis of the spread of chlamydial infections in the community. We have developed a multillocus sequence typing (MLST) system based on five target regions and compared it with analysis of ompA, the single gene most extensively used for genotyping. Sequence determination of 16 reference strains, comprising all major serotypes, serotypes A to L3, showed that the number of genetic variants in the five separate target regions ranged from 8 to 16. The genetic variation in 47 clinical C. trachomatis isolates of representative serotypes (14 serotype D, 12 serotype E, 11 serotype G, and 10 serotype K strains) was analyzed; and the MLST system detected 32 variants, whereas 12 variants were detected by using ompA analysis. Specimens of the predominant serotype, serotype E, were differentiated into seven genotypes by MLST but into only two by ompA analysis. The MLST system was applied to C. trachomatis specimens from a population of men who have sex with men and was able to differentiate 10 specimens of one predominant ompA genotype G variant into four distinct MLST variants. To conclude, our MLST system can be used to discriminate C. trachomatis strains and can be applied to high-resolution molecular epidemiology.},
  author       = {Klint, Markus and Fuxelius, Hans-Henrik and Rostlinger Goldkuhl, Renée and Skarin, Hanna and Rutemark, Christian and Andersson, Siv G E and Persson, Kenneth and Herrmann, Bjorn},
  issn         = {1098-660X},
  language     = {eng},
  number       = {5},
  pages        = {1410--1414},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Clinical Microbiology},
  title        = {High-Resolution Genotyping of Chlamydia trachomatis Strains by Multilocus Sequence Analysis.},
  url          = {http://dx.doi.org/10.1128/JCM.02301-06},
  volume       = {45},
  year         = {2007},
}