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Immunohistochemical Distribution of Cyclic GMP-Dependent Protein Kinase-1 in Human Prostate Tissue.

Waldkirch, Eginhard S; Uckert, Stefan; Langnase, Kristina; Richter, Karin; Jonas, Udo; Wolf, Gerald; Andersson, Karl-Erik LU ; Stief, Christian G and Hedlund, Petter LU (2007) In European Urology 52(2). p.495-502
Abstract
Objectives: Phosphodiesterase 5 (PDE5) inhibitors improve smooth muscle relaxation and therefore are considered for pharmacotherapy of benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS). Cyclic guanosine monophosphate (cGMP) -dependent protein kinase-1 (cGKI) has been identified as one of the downstream targets for cGMP. The aim of the present study was to evaluate, by means of immunohistochemistry and Western blot analysis, the expression and localization of cGKI isoforms in relation to smooth muscle alpha-actin and cGMP in the human prostate. Methods: Cryostat sections of tissue segments excised from the transition zone of human prostates from 11 patients (aged 54-68 yr) were incubated with primary antibodies... (More)
Objectives: Phosphodiesterase 5 (PDE5) inhibitors improve smooth muscle relaxation and therefore are considered for pharmacotherapy of benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS). Cyclic guanosine monophosphate (cGMP) -dependent protein kinase-1 (cGKI) has been identified as one of the downstream targets for cGMP. The aim of the present study was to evaluate, by means of immunohistochemistry and Western blot analysis, the expression and localization of cGKI isoforms in relation to smooth muscle alpha-actin and cGMP in the human prostate. Methods: Cryostat sections of tissue segments excised from the transition zone of human prostates from 11 patients (aged 54-68 yr) were incubated with primary antibodies directed against smooth muscle alpha-actin, cGMP, cGKI, c:GKI alpha, and cGKI beta. Visualization of double-labelled immunofluorescent staining was achieved by laser microscopy. Western blot analysis was performed to confirm the expression of cGKI isoforms. Results: Immunoreactivities specific for cGKI, cGKI alpha, and cGKI beta were observed in the smooth musculature of the transition zone. Double-staining revealed the colocalization of smooth muscle alpha-actin, cGMP, and cGKI isoforms in smooth muscle cells of the fibromuscular stroma. The expression of cGKI isoforms was confirmed by Western blot analysis. Conclusions: Our results confirm the presence of cGKI isoforms a and P in the transition zone of human prostate tissue. in addition, the colocalization of alpha-actin, cGMP, and cGKI isoforms provides further evidence for a significant role of the nitric oxide/cGMP pathway in the regulation of smooth muscle contractility in human prostate tissue and therefore could provide additional targets for pharmacotherapy of BPH and LUTS. (C) 2007 European Association of Urology. Published by Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cyclic nucleotides, benign prostatic hyperplasia, lower urinary tract, symptoms, phosphodiesterase 5, phosphodiesterase 5 inhibitor, protein, kinase G
in
European Urology
volume
52
issue
2
pages
495 - 502
publisher
Elsevier
external identifiers
  • wos:000248438900028
  • scopus:34250774010
ISSN
1873-7560
DOI
10.1016/j.eururo.2007.02.004
language
English
LU publication?
yes
id
88a79859-4f31-42b9-97bf-bfa70bf08b0a (old id 166942)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17329019&dopt=Abstract
date added to LUP
2007-07-06 10:11:45
date last changed
2017-02-19 04:16:10
@article{88a79859-4f31-42b9-97bf-bfa70bf08b0a,
  abstract     = {Objectives: Phosphodiesterase 5 (PDE5) inhibitors improve smooth muscle relaxation and therefore are considered for pharmacotherapy of benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS). Cyclic guanosine monophosphate (cGMP) -dependent protein kinase-1 (cGKI) has been identified as one of the downstream targets for cGMP. The aim of the present study was to evaluate, by means of immunohistochemistry and Western blot analysis, the expression and localization of cGKI isoforms in relation to smooth muscle alpha-actin and cGMP in the human prostate. Methods: Cryostat sections of tissue segments excised from the transition zone of human prostates from 11 patients (aged 54-68 yr) were incubated with primary antibodies directed against smooth muscle alpha-actin, cGMP, cGKI, c:GKI alpha, and cGKI beta. Visualization of double-labelled immunofluorescent staining was achieved by laser microscopy. Western blot analysis was performed to confirm the expression of cGKI isoforms. Results: Immunoreactivities specific for cGKI, cGKI alpha, and cGKI beta were observed in the smooth musculature of the transition zone. Double-staining revealed the colocalization of smooth muscle alpha-actin, cGMP, and cGKI isoforms in smooth muscle cells of the fibromuscular stroma. The expression of cGKI isoforms was confirmed by Western blot analysis. Conclusions: Our results confirm the presence of cGKI isoforms a and P in the transition zone of human prostate tissue. in addition, the colocalization of alpha-actin, cGMP, and cGKI isoforms provides further evidence for a significant role of the nitric oxide/cGMP pathway in the regulation of smooth muscle contractility in human prostate tissue and therefore could provide additional targets for pharmacotherapy of BPH and LUTS. (C) 2007 European Association of Urology. Published by Elsevier B.V. All rights reserved.},
  author       = {Waldkirch, Eginhard S and Uckert, Stefan and Langnase, Kristina and Richter, Karin and Jonas, Udo and Wolf, Gerald and Andersson, Karl-Erik and Stief, Christian G and Hedlund, Petter},
  issn         = {1873-7560},
  keyword      = {cyclic nucleotides,benign prostatic hyperplasia,lower urinary tract,symptoms,phosphodiesterase 5,phosphodiesterase 5 inhibitor,protein,kinase G},
  language     = {eng},
  number       = {2},
  pages        = {495--502},
  publisher    = {Elsevier},
  series       = {European Urology},
  title        = {Immunohistochemical Distribution of Cyclic GMP-Dependent Protein Kinase-1 in Human Prostate Tissue.},
  url          = {http://dx.doi.org/10.1016/j.eururo.2007.02.004},
  volume       = {52},
  year         = {2007},
}