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The Arabidopsis 14-3-3 family -target protein specificity and expression of isoforms

Arkell, Annika LU (2010)
Abstract
14-3-3 proteins comprise a family of highly conserved proteins. 14-3-3 proteins have been found in all organisms examined except for members of the prokaryotic kingdom. 14-3-3s are involved in numerous processes in the cell and they typically bind to phosphorylated motifs in other proteins and regulate their activities.

In plants, 14-3-3 proteins are recognized as key regulators of primary metabolism and membrane transport. In Arabidopsis, there are 15 genes coding for 14-3-3s and hence several 14-3-3 isoforms may be present simultaneously in the plant. The aim of my work has been to understand why there are so many 14-3-3 isoforms.

To investigate if there is specificity in 14-3-3/target protein interaction, the... (More)
14-3-3 proteins comprise a family of highly conserved proteins. 14-3-3 proteins have been found in all organisms examined except for members of the prokaryotic kingdom. 14-3-3s are involved in numerous processes in the cell and they typically bind to phosphorylated motifs in other proteins and regulate their activities.

In plants, 14-3-3 proteins are recognized as key regulators of primary metabolism and membrane transport. In Arabidopsis, there are 15 genes coding for 14-3-3s and hence several 14-3-3 isoforms may be present simultaneously in the plant. The aim of my work has been to understand why there are so many 14-3-3 isoforms.

To investigate if there is specificity in 14-3-3/target protein interaction, the H+-ATPase/14-3-3 interaction was used as a model system. The study indicated some specificity but also a wide redundancy. To further analyse the question of specificity at different levels promoter:GUS fusions were utilized. The results clearly indicate a developmental, cell-, tissue- and organ-specific expression for all of the 14-3-3 isoforms in Arabidopsis. There is not a single case where the promoter of one isoform shows an expression that is identical to the expression of another isoform. (Less)
Please use this url to cite or link to this publication:
author
supervisor
organization
publishing date
type
Thesis
publication status
published
subject
keywords
protein-protein interaction, GUS, Arabidopsis, 14-3-3, H+-ATPase, expression
pages
61 pages
publisher
Lunds universitet
ISBN
978-91-7422-254-8
language
English
LU publication?
yes
id
32760e0a-4024-44c7-9054-c968fb82c7fc (old id 1669922)
date added to LUP
2010-09-21 15:03:14
date last changed
2016-09-19 08:45:11
@misc{32760e0a-4024-44c7-9054-c968fb82c7fc,
  abstract     = {14-3-3 proteins comprise a family of highly conserved proteins. 14-3-3 proteins have been found in all organisms examined except for members of the prokaryotic kingdom. 14-3-3s are involved in numerous processes in the cell and they typically bind to phosphorylated motifs in other proteins and regulate their activities. <br/><br>
In plants, 14-3-3 proteins are recognized as key regulators of primary metabolism and membrane transport. In Arabidopsis, there are 15 genes coding for 14-3-3s and hence several 14-3-3 isoforms may be present simultaneously in the plant. The aim of my work has been to understand why there are so many 14-3-3 isoforms. <br/><br>
To investigate if there is specificity in 14-3-3/target protein interaction, the H+-ATPase/14-3-3 interaction was used as a model system. The study indicated some specificity but also a wide redundancy. To further analyse the question of specificity at different levels promoter:GUS fusions were utilized. The results clearly indicate a developmental, cell-, tissue- and organ-specific expression for all of the 14-3-3 isoforms in Arabidopsis. There is not a single case where the promoter of one isoform shows an expression that is identical to the expression of another isoform.},
  author       = {Arkell, Annika},
  isbn         = {978-91-7422-254-8},
  keyword      = {protein-protein interaction,GUS,Arabidopsis,14-3-3,H+-ATPase,expression},
  language     = {eng},
  note         = {Licentiate Thesis},
  pages        = {61},
  publisher    = {Lunds universitet},
  title        = {The Arabidopsis 14-3-3 family -target protein specificity and expression of isoforms},
  year         = {2010},
}