Advanced

Host Imprints on Bacterial Genomes-Rapid, Divergent Evolution in Individual Patients

Zdziarski, Jaroslaw; Brzuszkiewicz, Elzbieta; Wullt, Björn LU ; Liesegang, Heiko; Biran, Dvora; Voigt, Birgit; Grönberg Hernandez, Jenny LU ; Ragnarsdottir, Bryndis LU ; Hecker, Michael and Ron, Eliora Z., et al. (2010) In PLoS Pathogens 6(8).
Abstract
Bacteria lose or gain genetic material and through selection, new variants become fixed in the population. Here we provide the first, genome-wide example of a single bacterial strain's evolution in different deliberately colonized patients and the surprising insight that hosts appear to personalize their microflora. By first obtaining the complete genome sequence of the prototype asymptomatic bacteriuria strain E. coli 83972 and then resequencing its descendants after therapeutic bladder colonization of different patients, we identified 34 mutations, which affected metabolic and virulence-related genes. Further transcriptome and proteome analysis proved that these genome changes altered bacterial gene expression resulting in unique... (More)
Bacteria lose or gain genetic material and through selection, new variants become fixed in the population. Here we provide the first, genome-wide example of a single bacterial strain's evolution in different deliberately colonized patients and the surprising insight that hosts appear to personalize their microflora. By first obtaining the complete genome sequence of the prototype asymptomatic bacteriuria strain E. coli 83972 and then resequencing its descendants after therapeutic bladder colonization of different patients, we identified 34 mutations, which affected metabolic and virulence-related genes. Further transcriptome and proteome analysis proved that these genome changes altered bacterial gene expression resulting in unique adaptation patterns in each patient. Our results provide evidence that, in addition to stochastic events, adaptive bacterial evolution is driven by individual host environments. Ongoing loss of gene function supports the hypothesis that evolution towards commensalism rather than virulence is favored during asymptomatic bladder colonization. (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PLoS Pathogens
volume
6
issue
8
publisher
Public Library of Science
external identifiers
  • wos:000281399900048
  • scopus:77958120655
ISSN
1553-7366
DOI
10.1371/journal.ppat.1001078
language
English
LU publication?
yes
id
f1a87f45-3b1d-4b0f-b0f9-5f8a0ee33846 (old id 1672191)
date added to LUP
2010-09-21 15:26:21
date last changed
2018-06-17 03:33:13
@article{f1a87f45-3b1d-4b0f-b0f9-5f8a0ee33846,
  abstract     = {Bacteria lose or gain genetic material and through selection, new variants become fixed in the population. Here we provide the first, genome-wide example of a single bacterial strain's evolution in different deliberately colonized patients and the surprising insight that hosts appear to personalize their microflora. By first obtaining the complete genome sequence of the prototype asymptomatic bacteriuria strain E. coli 83972 and then resequencing its descendants after therapeutic bladder colonization of different patients, we identified 34 mutations, which affected metabolic and virulence-related genes. Further transcriptome and proteome analysis proved that these genome changes altered bacterial gene expression resulting in unique adaptation patterns in each patient. Our results provide evidence that, in addition to stochastic events, adaptive bacterial evolution is driven by individual host environments. Ongoing loss of gene function supports the hypothesis that evolution towards commensalism rather than virulence is favored during asymptomatic bladder colonization.},
  author       = {Zdziarski, Jaroslaw and Brzuszkiewicz, Elzbieta and Wullt, Björn and Liesegang, Heiko and Biran, Dvora and Voigt, Birgit and Grönberg Hernandez, Jenny and Ragnarsdottir, Bryndis and Hecker, Michael and Ron, Eliora Z. and Daniel, Rolf and Gottschalk, Gerhard and Hacker, Joerg and Svanborg, Catharina and Dobrindt, Ulrich},
  issn         = {1553-7366},
  language     = {eng},
  number       = {8},
  publisher    = {Public Library of Science},
  series       = {PLoS Pathogens},
  title        = {Host Imprints on Bacterial Genomes-Rapid, Divergent Evolution in Individual Patients},
  url          = {http://dx.doi.org/10.1371/journal.ppat.1001078},
  volume       = {6},
  year         = {2010},
}