Production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136
(2010) In Journal of Applied Microbiology 109(3). p.936-945- Abstract
- Aim: This study focuses on the production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136. Methods and Results: A Ser. proteamaculans strain producing a novel cold-active antimicrobial was isolated from Isla de los Estados, Argentina. Antimicrobial production was optimized in a BIOFLO 101 bioreactor under batch culture mode, with temperature, pH and dissolved oxygen controlled conditions. A purification protocol was developed including activated charcoal adsorption, solid-phase C18 extraction (SPE) and semi-preparative HPLC. The molecular weight was determined by LC/QTOF/MS/MS mass analysis. Conclusions: Serratia proteamaculans 136 produces a cold-active low... (More)
- Aim: This study focuses on the production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136. Methods and Results: A Ser. proteamaculans strain producing a novel cold-active antimicrobial was isolated from Isla de los Estados, Argentina. Antimicrobial production was optimized in a BIOFLO 101 bioreactor under batch culture mode, with temperature, pH and dissolved oxygen controlled conditions. A purification protocol was developed including activated charcoal adsorption, solid-phase C18 extraction (SPE) and semi-preparative HPLC. The molecular weight was determined by LC/QTOF/MS/MS mass analysis. Conclusions: Serratia proteamaculans 136 produces a cold-active low molecular bacteriocin-like compound named serraticin A. In this work, it has been laboratory-scale produced, purified and partially characterized. Cross-immunity test revealed that serraticin A is very different from other well-known microcins assayed, with a wide inhibitory spectrum, showing an interesting biotechnology potential to be applied as a control agent against pathogenic bacteria. Significance and Impact of the Study: The present study is the first report of a cold-active compound with antimicrobial activity from Ser. proteamaculans. The work also highlights that cold environments could be a suitable source of micro-organisms with ability to produce cold-active biomolecules of biotechnological interest. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1673701
- author
- Sanchez, L. A. ; Hedström, Martin LU ; Delgado, M. A. and Delgado, O. D.
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- psychrotolerant, psychrophiles, antimicrobials, bacteriocins, proteamaculans, Serratia
- in
- Journal of Applied Microbiology
- volume
- 109
- issue
- 3
- pages
- 936 - 945
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000280979700020
- scopus:77955815946
- pmid:20408913
- ISSN
- 1364-5072
- DOI
- 10.1111/j.1365-2672.2010.04720.x
- language
- English
- LU publication?
- yes
- id
- dd44a7c0-cddb-4d7f-b56c-5a7cf9e7f6de (old id 1673701)
- date added to LUP
- 2016-04-01 10:34:28
- date last changed
- 2022-03-19 22:01:13
@article{dd44a7c0-cddb-4d7f-b56c-5a7cf9e7f6de,
abstract = {{Aim: This study focuses on the production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136. Methods and Results: A Ser. proteamaculans strain producing a novel cold-active antimicrobial was isolated from Isla de los Estados, Argentina. Antimicrobial production was optimized in a BIOFLO 101 bioreactor under batch culture mode, with temperature, pH and dissolved oxygen controlled conditions. A purification protocol was developed including activated charcoal adsorption, solid-phase C18 extraction (SPE) and semi-preparative HPLC. The molecular weight was determined by LC/QTOF/MS/MS mass analysis. Conclusions: Serratia proteamaculans 136 produces a cold-active low molecular bacteriocin-like compound named serraticin A. In this work, it has been laboratory-scale produced, purified and partially characterized. Cross-immunity test revealed that serraticin A is very different from other well-known microcins assayed, with a wide inhibitory spectrum, showing an interesting biotechnology potential to be applied as a control agent against pathogenic bacteria. Significance and Impact of the Study: The present study is the first report of a cold-active compound with antimicrobial activity from Ser. proteamaculans. The work also highlights that cold environments could be a suitable source of micro-organisms with ability to produce cold-active biomolecules of biotechnological interest.}},
author = {{Sanchez, L. A. and Hedström, Martin and Delgado, M. A. and Delgado, O. D.}},
issn = {{1364-5072}},
keywords = {{psychrotolerant; psychrophiles; antimicrobials; bacteriocins; proteamaculans; Serratia}},
language = {{eng}},
number = {{3}},
pages = {{936--945}},
publisher = {{Wiley-Blackwell}},
series = {{Journal of Applied Microbiology}},
title = {{Production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136}},
url = {{http://dx.doi.org/10.1111/j.1365-2672.2010.04720.x}},
doi = {{10.1111/j.1365-2672.2010.04720.x}},
volume = {{109}},
year = {{2010}},
}