Advanced

Production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136

Sanchez, L. A.; Hedström, Martin LU ; Delgado, M. A. and Delgado, O. D. (2010) In Journal of Applied Microbiology1997-01-01+01:00 109(3). p.936-945
Abstract
Aim: This study focuses on the production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136. Methods and Results: A Ser. proteamaculans strain producing a novel cold-active antimicrobial was isolated from Isla de los Estados, Argentina. Antimicrobial production was optimized in a BIOFLO 101 bioreactor under batch culture mode, with temperature, pH and dissolved oxygen controlled conditions. A purification protocol was developed including activated charcoal adsorption, solid-phase C18 extraction (SPE) and semi-preparative HPLC. The molecular weight was determined by LC/QTOF/MS/MS mass analysis. Conclusions: Serratia proteamaculans 136 produces a cold-active low... (More)
Aim: This study focuses on the production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136. Methods and Results: A Ser. proteamaculans strain producing a novel cold-active antimicrobial was isolated from Isla de los Estados, Argentina. Antimicrobial production was optimized in a BIOFLO 101 bioreactor under batch culture mode, with temperature, pH and dissolved oxygen controlled conditions. A purification protocol was developed including activated charcoal adsorption, solid-phase C18 extraction (SPE) and semi-preparative HPLC. The molecular weight was determined by LC/QTOF/MS/MS mass analysis. Conclusions: Serratia proteamaculans 136 produces a cold-active low molecular bacteriocin-like compound named serraticin A. In this work, it has been laboratory-scale produced, purified and partially characterized. Cross-immunity test revealed that serraticin A is very different from other well-known microcins assayed, with a wide inhibitory spectrum, showing an interesting biotechnology potential to be applied as a control agent against pathogenic bacteria. Significance and Impact of the Study: The present study is the first report of a cold-active compound with antimicrobial activity from Ser. proteamaculans. The work also highlights that cold environments could be a suitable source of micro-organisms with ability to produce cold-active biomolecules of biotechnological interest. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
psychrotolerant, psychrophiles, antimicrobials, bacteriocins, proteamaculans, Serratia
in
Journal of Applied Microbiology1997-01-01+01:00
volume
109
issue
3
pages
936 - 945
publisher
Wiley-Blackwell
external identifiers
  • wos:000280979700020
  • scopus:77955815946
ISSN
1364-5072
DOI
10.1111/j.1365-2672.2010.04720.x
language
English
LU publication?
yes
id
dd44a7c0-cddb-4d7f-b56c-5a7cf9e7f6de (old id 1673701)
date added to LUP
2010-09-23 08:17:23
date last changed
2018-07-15 03:14:00
@article{dd44a7c0-cddb-4d7f-b56c-5a7cf9e7f6de,
  abstract     = {Aim: This study focuses on the production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136. Methods and Results: A Ser. proteamaculans strain producing a novel cold-active antimicrobial was isolated from Isla de los Estados, Argentina. Antimicrobial production was optimized in a BIOFLO 101 bioreactor under batch culture mode, with temperature, pH and dissolved oxygen controlled conditions. A purification protocol was developed including activated charcoal adsorption, solid-phase C18 extraction (SPE) and semi-preparative HPLC. The molecular weight was determined by LC/QTOF/MS/MS mass analysis. Conclusions: Serratia proteamaculans 136 produces a cold-active low molecular bacteriocin-like compound named serraticin A. In this work, it has been laboratory-scale produced, purified and partially characterized. Cross-immunity test revealed that serraticin A is very different from other well-known microcins assayed, with a wide inhibitory spectrum, showing an interesting biotechnology potential to be applied as a control agent against pathogenic bacteria. Significance and Impact of the Study: The present study is the first report of a cold-active compound with antimicrobial activity from Ser. proteamaculans. The work also highlights that cold environments could be a suitable source of micro-organisms with ability to produce cold-active biomolecules of biotechnological interest.},
  author       = {Sanchez, L. A. and Hedström, Martin and Delgado, M. A. and Delgado, O. D.},
  issn         = {1364-5072},
  keyword      = {psychrotolerant,psychrophiles,antimicrobials,bacteriocins,proteamaculans,Serratia},
  language     = {eng},
  number       = {3},
  pages        = {936--945},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Applied Microbiology1997-01-01+01:00},
  title        = {Production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136},
  url          = {http://dx.doi.org/10.1111/j.1365-2672.2010.04720.x},
  volume       = {109},
  year         = {2010},
}