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Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: I. Analyte definition and proposal of a candidate reference method.

Jeppsson, Jan-Olof LU ; Arndt, Torsten; Schellenberg, François; Wielders, Jos P M; Anton, Raymond F; Whitfield, John B and Helander, Anders (2007) In Clinical Chemistry and Laboratory Medicine 45(4). p.558-562
Abstract
An alcohol-associated change in the serum transferrin glycoform pattern, carbohydrate-deficient transferrin (CDT), is used as a biomarker of chronic moderate to heavy alcohol consumption. A current limitation in CDT analysis is the lack of standardization, which hampers clinical and analytical comparison between studies. This situation prompted initiation of a Working Group (WG) on CDT Standardization under the auspices of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). The standardization work aims to define and validate the analyte, select a reference method, work out procedures for the production of reference materials, and make suggestions for the clinical usage of CDT. The first recommendation of the... (More)
An alcohol-associated change in the serum transferrin glycoform pattern, carbohydrate-deficient transferrin (CDT), is used as a biomarker of chronic moderate to heavy alcohol consumption. A current limitation in CDT analysis is the lack of standardization, which hampers clinical and analytical comparison between studies. This situation prompted initiation of a Working Group (WG) on CDT Standardization under the auspices of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). The standardization work aims to define and validate the analyte, select a reference method, work out procedures for the production of reference materials, and make suggestions for the clinical usage of CDT. The first recommendation of the WG is that disialotransferrin should be the primary target molecule for CDT measurement and the single analyte on which CDT standardization is based. It is further recommended that HPLC should be the analytical principle considered as the basis of an interim reference method until a suitable mass spectrometric reference method is established. In clinical use, CDT should be expressed in a relative amount (% CDT), to compensate for variations in the total transferrin concentration. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
alcohol biomarker, HPLC, standardization, carbohydrate-deficient transferrin (CDT), disialotransferrin
in
Clinical Chemistry and Laboratory Medicine
volume
45
issue
4
pages
558 - 562
publisher
De Gruyter
external identifiers
  • wos:000246305100022
  • scopus:34247380374
ISSN
1434-6621
DOI
10.1515/CCLM.2007.107
language
English
LU publication?
yes
id
2f4f7416-cb7b-48d7-8e66-bb0fe0b40209 (old id 167590)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17439340&dopt=Abstract
date added to LUP
2007-07-25 14:02:56
date last changed
2017-09-10 03:32:01
@article{2f4f7416-cb7b-48d7-8e66-bb0fe0b40209,
  abstract     = {An alcohol-associated change in the serum transferrin glycoform pattern, carbohydrate-deficient transferrin (CDT), is used as a biomarker of chronic moderate to heavy alcohol consumption. A current limitation in CDT analysis is the lack of standardization, which hampers clinical and analytical comparison between studies. This situation prompted initiation of a Working Group (WG) on CDT Standardization under the auspices of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). The standardization work aims to define and validate the analyte, select a reference method, work out procedures for the production of reference materials, and make suggestions for the clinical usage of CDT. The first recommendation of the WG is that disialotransferrin should be the primary target molecule for CDT measurement and the single analyte on which CDT standardization is based. It is further recommended that HPLC should be the analytical principle considered as the basis of an interim reference method until a suitable mass spectrometric reference method is established. In clinical use, CDT should be expressed in a relative amount (% CDT), to compensate for variations in the total transferrin concentration.},
  author       = {Jeppsson, Jan-Olof and Arndt, Torsten and Schellenberg, François and Wielders, Jos P M and Anton, Raymond F and Whitfield, John B and Helander, Anders},
  issn         = {1434-6621},
  keyword      = {alcohol biomarker,HPLC,standardization,carbohydrate-deficient transferrin (CDT),disialotransferrin},
  language     = {eng},
  number       = {4},
  pages        = {558--562},
  publisher    = {De Gruyter},
  series       = {Clinical Chemistry and Laboratory Medicine},
  title        = {Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: I. Analyte definition and proposal of a candidate reference method.},
  url          = {http://dx.doi.org/10.1515/CCLM.2007.107},
  volume       = {45},
  year         = {2007},
}