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The CD53 and CEACAM-1 genes are genetic targets for early B cell factor.

Månsson, Robert LU ; Lagergren, Anna LU ; Hansson, Frida; Smith, Emma LU and Sigvardsson, Mikael LU (2007) In European Journal of Immunology 37(5). p.1365-1376
Abstract
Early B cell factor (EBF)-1 is a transcription factor known to be of critical importance for early B lymphocyte development. EBF-1 has been shown to directly interact with and regulate expression of a set of genes involved in the functional formation of the preB cell receptor, but the dramatic phenotype observed in the EBF-1-deficient mice suggests that several additional genes are activated by this protein. In order to identify additional target genes for EBF-1, we transduced a hematopoietic progenitor cell line, BaF/3, with an EBF-1-encoding retrovirus and investigated the induced gene expression pattern by micro-arrays. This analysis suggested that among others, the CD53 and the carcinoembryonic antigen-related cell adhesion molecule... (More)
Early B cell factor (EBF)-1 is a transcription factor known to be of critical importance for early B lymphocyte development. EBF-1 has been shown to directly interact with and regulate expression of a set of genes involved in the functional formation of the preB cell receptor, but the dramatic phenotype observed in the EBF-1-deficient mice suggests that several additional genes are activated by this protein. In order to identify additional target genes for EBF-1, we transduced a hematopoietic progenitor cell line, BaF/3, with an EBF-1-encoding retrovirus and investigated the induced gene expression pattern by micro-arrays. This analysis suggested that among others, the CD53 and the carcinoembryonic antigen-related cell adhesion molecule (CEACAM)-1 genes both were induced by ectopic expression of EBF-1. Identification of the 5' end of the cDNA enabled the identification of promoter elements with functional binding sites for EBF-1 and ability to respond to EBF-1 expression in transient transfection assays. These data suggest that CD53 and CEACAM-1 are direct genetic targets for EBF-1, providing additional information concerning the activity of this crucial transcription factor in hematopoiesis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Immunology
volume
37
issue
5
pages
1365 - 1376
publisher
John Wiley & Sons
external identifiers
  • wos:000246558500025
  • scopus:34250327075
ISSN
1521-4141
DOI
10.1002/eji.200636642
language
English
LU publication?
yes
id
c21d3b63-ca7f-4823-b7a6-745beb862368 (old id 167680)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17429843&dopt=Abstract
date added to LUP
2007-07-24 09:07:45
date last changed
2017-01-01 05:14:49
@article{c21d3b63-ca7f-4823-b7a6-745beb862368,
  abstract     = {Early B cell factor (EBF)-1 is a transcription factor known to be of critical importance for early B lymphocyte development. EBF-1 has been shown to directly interact with and regulate expression of a set of genes involved in the functional formation of the preB cell receptor, but the dramatic phenotype observed in the EBF-1-deficient mice suggests that several additional genes are activated by this protein. In order to identify additional target genes for EBF-1, we transduced a hematopoietic progenitor cell line, BaF/3, with an EBF-1-encoding retrovirus and investigated the induced gene expression pattern by micro-arrays. This analysis suggested that among others, the CD53 and the carcinoembryonic antigen-related cell adhesion molecule (CEACAM)-1 genes both were induced by ectopic expression of EBF-1. Identification of the 5' end of the cDNA enabled the identification of promoter elements with functional binding sites for EBF-1 and ability to respond to EBF-1 expression in transient transfection assays. These data suggest that CD53 and CEACAM-1 are direct genetic targets for EBF-1, providing additional information concerning the activity of this crucial transcription factor in hematopoiesis.},
  author       = {Månsson, Robert and Lagergren, Anna and Hansson, Frida and Smith, Emma and Sigvardsson, Mikael},
  issn         = {1521-4141},
  language     = {eng},
  number       = {5},
  pages        = {1365--1376},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Immunology},
  title        = {The CD53 and CEACAM-1 genes are genetic targets for early B cell factor.},
  url          = {http://dx.doi.org/10.1002/eji.200636642},
  volume       = {37},
  year         = {2007},
}