A molecular map of murine lymph node blood vascular endothelium at single cell resolution
(2020) In Nature Communications 11(1).- Abstract
Blood vascular endothelial cells (BECs) control the immune response by regulating blood flow and immune cell recruitment in lymphoid tissues. However, the diversity of BEC and their origins during immune angiogenesis remain unclear. Here we profile transcriptomes of BEC from peripheral lymph nodes and map phenotypes to the vasculature. We identify multiple subsets, including a medullary venous population whose gene signature predicts a selective role in myeloid cell (vs lymphocyte) recruitment to the medulla, confirmed by videomicroscopy. We define five capillary subsets, including a capillary resident precursor (CRP) that displays stem cell and migratory gene signatures, and contributes to homeostatic BEC turnover and to neogenesis of... (More)
Blood vascular endothelial cells (BECs) control the immune response by regulating blood flow and immune cell recruitment in lymphoid tissues. However, the diversity of BEC and their origins during immune angiogenesis remain unclear. Here we profile transcriptomes of BEC from peripheral lymph nodes and map phenotypes to the vasculature. We identify multiple subsets, including a medullary venous population whose gene signature predicts a selective role in myeloid cell (vs lymphocyte) recruitment to the medulla, confirmed by videomicroscopy. We define five capillary subsets, including a capillary resident precursor (CRP) that displays stem cell and migratory gene signatures, and contributes to homeostatic BEC turnover and to neogenesis of high endothelium after immunization. Cell alignments show retention of developmental programs along trajectories from CRP to mature venous and arterial populations. Our single cell atlas provides a molecular roadmap of the lymph node blood vasculature and defines subset specialization for leukocyte recruitment and vascular homeostasis.
(Less)
- author
- publishing date
- 2020-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nature Communications
- volume
- 11
- issue
- 1
- article number
- 3798
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85088791783
- pmid:32732867
- ISSN
- 2041-1723
- DOI
- 10.1038/s41467-020-17291-5
- language
- English
- LU publication?
- no
- id
- 167e95d4-756a-437c-8db0-d2020b511833
- date added to LUP
- 2020-10-09 09:55:03
- date last changed
- 2024-04-17 16:04:24
@article{167e95d4-756a-437c-8db0-d2020b511833, abstract = {{<p>Blood vascular endothelial cells (BECs) control the immune response by regulating blood flow and immune cell recruitment in lymphoid tissues. However, the diversity of BEC and their origins during immune angiogenesis remain unclear. Here we profile transcriptomes of BEC from peripheral lymph nodes and map phenotypes to the vasculature. We identify multiple subsets, including a medullary venous population whose gene signature predicts a selective role in myeloid cell (vs lymphocyte) recruitment to the medulla, confirmed by videomicroscopy. We define five capillary subsets, including a capillary resident precursor (CRP) that displays stem cell and migratory gene signatures, and contributes to homeostatic BEC turnover and to neogenesis of high endothelium after immunization. Cell alignments show retention of developmental programs along trajectories from CRP to mature venous and arterial populations. Our single cell atlas provides a molecular roadmap of the lymph node blood vasculature and defines subset specialization for leukocyte recruitment and vascular homeostasis.</p>}}, author = {{Brulois, Kevin and Rajaraman, Anusha and Szade, Agata and Nordling, Sofia and Bogoslowski, Ania and Dermadi, Denis and Rahman, Milladur and Kiefel, Helena and O’Hara, Edward and Koning, Jasper J. and Kawashima, Hiroto and Zhou, Bin and Vestweber, Dietmar and Red-Horse, Kristy and Mebius, Reina E. and Adams, Ralf H. and Kubes, Paul and Pan, Junliang and Butcher, Eugene C.}}, issn = {{2041-1723}}, language = {{eng}}, month = {{12}}, number = {{1}}, publisher = {{Nature Publishing Group}}, series = {{Nature Communications}}, title = {{A molecular map of murine lymph node blood vascular endothelium at single cell resolution}}, url = {{http://dx.doi.org/10.1038/s41467-020-17291-5}}, doi = {{10.1038/s41467-020-17291-5}}, volume = {{11}}, year = {{2020}}, }