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A molecular map of murine lymph node blood vascular endothelium at single cell resolution

Brulois, Kevin ; Rajaraman, Anusha ; Szade, Agata ; Nordling, Sofia ; Bogoslowski, Ania ; Dermadi, Denis ; Rahman, Milladur LU orcid ; Kiefel, Helena ; O’Hara, Edward and Koning, Jasper J. , et al. (2020) In Nature Communications 11(1).
Abstract

Blood vascular endothelial cells (BECs) control the immune response by regulating blood flow and immune cell recruitment in lymphoid tissues. However, the diversity of BEC and their origins during immune angiogenesis remain unclear. Here we profile transcriptomes of BEC from peripheral lymph nodes and map phenotypes to the vasculature. We identify multiple subsets, including a medullary venous population whose gene signature predicts a selective role in myeloid cell (vs lymphocyte) recruitment to the medulla, confirmed by videomicroscopy. We define five capillary subsets, including a capillary resident precursor (CRP) that displays stem cell and migratory gene signatures, and contributes to homeostatic BEC turnover and to neogenesis of... (More)

Blood vascular endothelial cells (BECs) control the immune response by regulating blood flow and immune cell recruitment in lymphoid tissues. However, the diversity of BEC and their origins during immune angiogenesis remain unclear. Here we profile transcriptomes of BEC from peripheral lymph nodes and map phenotypes to the vasculature. We identify multiple subsets, including a medullary venous population whose gene signature predicts a selective role in myeloid cell (vs lymphocyte) recruitment to the medulla, confirmed by videomicroscopy. We define five capillary subsets, including a capillary resident precursor (CRP) that displays stem cell and migratory gene signatures, and contributes to homeostatic BEC turnover and to neogenesis of high endothelium after immunization. Cell alignments show retention of developmental programs along trajectories from CRP to mature venous and arterial populations. Our single cell atlas provides a molecular roadmap of the lymph node blood vasculature and defines subset specialization for leukocyte recruitment and vascular homeostasis.

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publishing date
type
Contribution to journal
publication status
published
subject
in
Nature Communications
volume
11
issue
1
article number
3798
publisher
Nature Publishing Group
external identifiers
  • scopus:85088791783
  • pmid:32732867
ISSN
2041-1723
DOI
10.1038/s41467-020-17291-5
language
English
LU publication?
no
id
167e95d4-756a-437c-8db0-d2020b511833
date added to LUP
2020-10-09 09:55:03
date last changed
2024-04-17 16:04:24
@article{167e95d4-756a-437c-8db0-d2020b511833,
  abstract     = {{<p>Blood vascular endothelial cells (BECs) control the immune response by regulating blood flow and immune cell recruitment in lymphoid tissues. However, the diversity of BEC and their origins during immune angiogenesis remain unclear. Here we profile transcriptomes of BEC from peripheral lymph nodes and map phenotypes to the vasculature. We identify multiple subsets, including a medullary venous population whose gene signature predicts a selective role in myeloid cell (vs lymphocyte) recruitment to the medulla, confirmed by videomicroscopy. We define five capillary subsets, including a capillary resident precursor (CRP) that displays stem cell and migratory gene signatures, and contributes to homeostatic BEC turnover and to neogenesis of high endothelium after immunization. Cell alignments show retention of developmental programs along trajectories from CRP to mature venous and arterial populations. Our single cell atlas provides a molecular roadmap of the lymph node blood vasculature and defines subset specialization for leukocyte recruitment and vascular homeostasis.</p>}},
  author       = {{Brulois, Kevin and Rajaraman, Anusha and Szade, Agata and Nordling, Sofia and Bogoslowski, Ania and Dermadi, Denis and Rahman, Milladur and Kiefel, Helena and O’Hara, Edward and Koning, Jasper J. and Kawashima, Hiroto and Zhou, Bin and Vestweber, Dietmar and Red-Horse, Kristy and Mebius, Reina E. and Adams, Ralf H. and Kubes, Paul and Pan, Junliang and Butcher, Eugene C.}},
  issn         = {{2041-1723}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Communications}},
  title        = {{A molecular map of murine lymph node blood vascular endothelium at single cell resolution}},
  url          = {{http://dx.doi.org/10.1038/s41467-020-17291-5}},
  doi          = {{10.1038/s41467-020-17291-5}},
  volume       = {{11}},
  year         = {{2020}},
}