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Characterization of an alternative transcript of the human CREB3L2 gene.

Panagopoulos, Ioannis LU ; Monsef, Nastaran LU ; Collin, Anna LU and Mertens, Fredrik LU (2010) In Oncology Reports 24(5). p.1133-1139
Abstract
CREB3L2, a member of the CREB3 family of transcription factors, spans >120 kbp and is composed of 12 exons. We characterized a widely expressed transcript of CREB3L2 generated by an intronic polyadenylation site in intron 4 of the gene. It could be translated to a CREB3L2 variant which is localized both in the nucleus and the endoplasmatic reticulum. The protein retains the N-terminal transactivation domain but lacks the DNA-binding domain, the transmembrane domain and the C-terminal part. Experiments using a GAL4 DNA-binding domain fusion model showed that the transcript is a transactivator but it cannot exert its function through the CRE and ATF6 binding sites and has little effect on the GRP78 promoter. Whether this transcript has a... (More)
CREB3L2, a member of the CREB3 family of transcription factors, spans >120 kbp and is composed of 12 exons. We characterized a widely expressed transcript of CREB3L2 generated by an intronic polyadenylation site in intron 4 of the gene. It could be translated to a CREB3L2 variant which is localized both in the nucleus and the endoplasmatic reticulum. The protein retains the N-terminal transactivation domain but lacks the DNA-binding domain, the transmembrane domain and the C-terminal part. Experiments using a GAL4 DNA-binding domain fusion model showed that the transcript is a transactivator but it cannot exert its function through the CRE and ATF6 binding sites and has little effect on the GRP78 promoter. Whether this transcript has a cellular function or is targeted for degradation by nonsense-mediated RNA decay system of RNA surveillance is currently unknown. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Oncology Reports
volume
24
issue
5
pages
1133 - 1139
publisher
D.A. Spandidos
external identifiers
  • WOS:000283112200003
  • PMID:20878102
  • Scopus:78149305119
ISSN
1791-2431
DOI
10.3892/or_00000964
language
English
LU publication?
yes
id
bf07991f-e9d3-4d6d-b176-478971417b0a (old id 1687677)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20878102?dopt=Abstract
date added to LUP
2010-10-07 15:35:41
date last changed
2017-01-01 07:45:33
@article{bf07991f-e9d3-4d6d-b176-478971417b0a,
  abstract     = {CREB3L2, a member of the CREB3 family of transcription factors, spans >120 kbp and is composed of 12 exons. We characterized a widely expressed transcript of CREB3L2 generated by an intronic polyadenylation site in intron 4 of the gene. It could be translated to a CREB3L2 variant which is localized both in the nucleus and the endoplasmatic reticulum. The protein retains the N-terminal transactivation domain but lacks the DNA-binding domain, the transmembrane domain and the C-terminal part. Experiments using a GAL4 DNA-binding domain fusion model showed that the transcript is a transactivator but it cannot exert its function through the CRE and ATF6 binding sites and has little effect on the GRP78 promoter. Whether this transcript has a cellular function or is targeted for degradation by nonsense-mediated RNA decay system of RNA surveillance is currently unknown.},
  author       = {Panagopoulos, Ioannis and Monsef, Nastaran and Collin, Anna and Mertens, Fredrik},
  issn         = {1791-2431},
  language     = {eng},
  number       = {5},
  pages        = {1133--1139},
  publisher    = {D.A. Spandidos},
  series       = {Oncology Reports},
  title        = {Characterization of an alternative transcript of the human CREB3L2 gene.},
  url          = {http://dx.doi.org/10.3892/or_00000964},
  volume       = {24},
  year         = {2010},
}