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Ultrasensitive Detection of HIV-1 p24 Antigen Using Nanofunctionalized Surfaces in a Capacitive Immunosensor.

Teeparuksapun, Kosin LU ; Hedström, Martin LU ; Wong, Eric Y; Tang, Shixing; Hewlett, Indira K and Mattiasson, Bo LU (2010) In Analytical Chemistry 82(20). p.8406-8411
Abstract
The HIV-1 capsid protein, p24 antigen, is of considerable diagnostic interest because following HIV exposure it is detectable several days earlier than host-generated HIV antibodies (which are the target of almost all current tests used in the field) and can be used to design very sensitive assays without the need for PCR. Here, we present an ultrasensitive capacitive immunosensor that is capable of detecting subattogram per milliliter concentrations of p24 antigen, which to our knowledge is the lowest level of detection ever reported. Dilution studies using p24-spiked human plasma samples indicate that the immunosensor is robust against the interfering effects of a complex biological matrix. Moreover, the capacitive immunosensor assay is... (More)
The HIV-1 capsid protein, p24 antigen, is of considerable diagnostic interest because following HIV exposure it is detectable several days earlier than host-generated HIV antibodies (which are the target of almost all current tests used in the field) and can be used to design very sensitive assays without the need for PCR. Here, we present an ultrasensitive capacitive immunosensor that is capable of detecting subattogram per milliliter concentrations of p24 antigen, which to our knowledge is the lowest level of detection ever reported. Dilution studies using p24-spiked human plasma samples indicate that the immunosensor is robust against the interfering effects of a complex biological matrix. Moreover, the capacitive immunosensor assay is rapid (<20 min), label-free, and generates data in real-time, with a portable format in development. Additional optimization of the capture agents and/or surface chemistries may further improve performance, highlighting the potential of this platform to serve as a diagnostic tool for early detection of HIV in field settings. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
82
issue
20
pages
8406 - 8411
publisher
The American Chemical Society
external identifiers
  • wos:000282859100009
  • pmid:20873850
  • scopus:77958031676
ISSN
1520-6882
DOI
10.1021/ac102144a
language
English
LU publication?
yes
id
9f46437a-bfcc-409f-a75e-44db45c2557c (old id 1687764)
date added to LUP
2010-10-15 12:58:17
date last changed
2018-06-10 03:15:35
@article{9f46437a-bfcc-409f-a75e-44db45c2557c,
  abstract     = {The HIV-1 capsid protein, p24 antigen, is of considerable diagnostic interest because following HIV exposure it is detectable several days earlier than host-generated HIV antibodies (which are the target of almost all current tests used in the field) and can be used to design very sensitive assays without the need for PCR. Here, we present an ultrasensitive capacitive immunosensor that is capable of detecting subattogram per milliliter concentrations of p24 antigen, which to our knowledge is the lowest level of detection ever reported. Dilution studies using p24-spiked human plasma samples indicate that the immunosensor is robust against the interfering effects of a complex biological matrix. Moreover, the capacitive immunosensor assay is rapid (&lt;20 min), label-free, and generates data in real-time, with a portable format in development. Additional optimization of the capture agents and/or surface chemistries may further improve performance, highlighting the potential of this platform to serve as a diagnostic tool for early detection of HIV in field settings.},
  author       = {Teeparuksapun, Kosin and Hedström, Martin and Wong, Eric Y and Tang, Shixing and Hewlett, Indira K and Mattiasson, Bo},
  issn         = {1520-6882},
  language     = {eng},
  number       = {20},
  pages        = {8406--8411},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Ultrasensitive Detection of HIV-1 p24 Antigen Using Nanofunctionalized Surfaces in a Capacitive Immunosensor.},
  url          = {http://dx.doi.org/10.1021/ac102144a},
  volume       = {82},
  year         = {2010},
}