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Integrated set up of sampling; sample treatment and assay of an intracellular protein during fermentation.

Mazlomi, Mohammad LU ; Hedström, Martin LU and Mattiasson, Bo LU (2010) In Journal of Biotechnology 150(3). p.366-371
Abstract
A complete system for on-line sampling, sample handling and analysis of fermentation processes has been set up. The system is designed such that a sample is taken from the fermentor, the metabolism of microbial cells is instantaneously arrested, the cells are lysed in order to release intracellular compounds and in a subsequent binding assay the content of target molecules is monitored. The system is constructed based on the flow-injection analysis concept. Good agreement between on-line analysis and off-line analysis is reported.
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biotechnology
volume
150
issue
3
pages
366 - 371
publisher
Elsevier
external identifiers
  • wos:000284984300013
  • pmid:20869403
  • scopus:78149466552
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2010.09.937
language
English
LU publication?
yes
id
14f53ea7-a66a-4cec-b1df-d68fb710f21f (old id 1687808)
date added to LUP
2016-04-01 10:14:36
date last changed
2022-01-25 21:16:40
@article{14f53ea7-a66a-4cec-b1df-d68fb710f21f,
  abstract     = {{A complete system for on-line sampling, sample handling and analysis of fermentation processes has been set up. The system is designed such that a sample is taken from the fermentor, the metabolism of microbial cells is instantaneously arrested, the cells are lysed in order to release intracellular compounds and in a subsequent binding assay the content of target molecules is monitored. The system is constructed based on the flow-injection analysis concept. Good agreement between on-line analysis and off-line analysis is reported.}},
  author       = {{Mazlomi, Mohammad and Hedström, Martin and Mattiasson, Bo}},
  issn         = {{1873-4863}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{366--371}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Biotechnology}},
  title        = {{Integrated set up of sampling; sample treatment and assay of an intracellular protein during fermentation.}},
  url          = {{http://dx.doi.org/10.1016/j.jbiotec.2010.09.937}},
  doi          = {{10.1016/j.jbiotec.2010.09.937}},
  volume       = {{150}},
  year         = {{2010}},
}