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Biochemical and functional correlates of an increased membrane density of caveolae in hypertrophic rat urinary bladder.

Shakirova, Yulia LU ; Swärd, Karl LU ; Uvelius, Bengt LU and Ekman, Mari LU (2010) In European Journal of Pharmacology 649. p.362-368
Abstract
Organ hypertrophy is often found to be associated with changes in the expression of caveolins and altered density of caveolae in the membrane. A plethora of signalling intermediaries are associated with caveolae and loss of caveolae has profound effects on contractility of the urinary bladder. We hypothesized that smooth muscle hypertrophy caused by bladder outflow obstruction (BOO) might lead to an altered caveola density with consequences for contractile regulation. Rat BOO for 6weeks caused a 2.56-fold increase in the number of smooth muscle caveolae per μm membrane. No changes in the expression of caveolin-1 or cavin-1, normalized to β-actin were seen, but membrane area per unit muscle volume dropped to 0.346. Hypertrophy was... (More)
Organ hypertrophy is often found to be associated with changes in the expression of caveolins and altered density of caveolae in the membrane. A plethora of signalling intermediaries are associated with caveolae and loss of caveolae has profound effects on contractility of the urinary bladder. We hypothesized that smooth muscle hypertrophy caused by bladder outflow obstruction (BOO) might lead to an altered caveola density with consequences for contractile regulation. Rat BOO for 6weeks caused a 2.56-fold increase in the number of smooth muscle caveolae per μm membrane. No changes in the expression of caveolin-1 or cavin-1, normalized to β-actin were seen, but membrane area per unit muscle volume dropped to 0.346. Hypertrophy was associated with altered contraction in response to carbachol. The effect on contraction of cholesterol desorption, which disrupts lipid rafts and caveolae, was however not changed. Contraction in response to bradykinin resisted mβcd in control destrusor, but was inhibited by it after 6weeks of obstruction. It is concluded that rat detrusor hypertrophy leads to an increased number of caveolae per unit membrane area. This change is due to a reduction of membrane area per volume muscle and it does not play a role for cholinergic activation, but promotes contraction in response to bradykinin after long-term obstruction. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Pharmacology
volume
649
pages
362 - 368
publisher
Elsevier
external identifiers
  • wos:000284294000050
  • pmid:20868660
  • scopus:78049282840
ISSN
1879-0712
DOI
10.1016/j.ejphar.2010.09.050
language
English
LU publication?
yes
id
6bd8e7dc-e5ca-4afd-ab63-3922a88f751b (old id 1687821)
alternative location
http://lup.lub.lu.se/record/1687821/file/1693747.pdf
http://www.ncbi.nlm.nih.gov/pubmed/20868660?dopt=Abstract
date added to LUP
2010-10-08 09:16:23
date last changed
2018-06-24 04:48:35
@article{6bd8e7dc-e5ca-4afd-ab63-3922a88f751b,
  abstract     = {Organ hypertrophy is often found to be associated with changes in the expression of caveolins and altered density of caveolae in the membrane. A plethora of signalling intermediaries are associated with caveolae and loss of caveolae has profound effects on contractility of the urinary bladder. We hypothesized that smooth muscle hypertrophy caused by bladder outflow obstruction (BOO) might lead to an altered caveola density with consequences for contractile regulation. Rat BOO for 6weeks caused a 2.56-fold increase in the number of smooth muscle caveolae per μm membrane. No changes in the expression of caveolin-1 or cavin-1, normalized to β-actin were seen, but membrane area per unit muscle volume dropped to 0.346. Hypertrophy was associated with altered contraction in response to carbachol. The effect on contraction of cholesterol desorption, which disrupts lipid rafts and caveolae, was however not changed. Contraction in response to bradykinin resisted mβcd in control destrusor, but was inhibited by it after 6weeks of obstruction. It is concluded that rat detrusor hypertrophy leads to an increased number of caveolae per unit membrane area. This change is due to a reduction of membrane area per volume muscle and it does not play a role for cholinergic activation, but promotes contraction in response to bradykinin after long-term obstruction.},
  author       = {Shakirova, Yulia and Swärd, Karl and Uvelius, Bengt and Ekman, Mari},
  issn         = {1879-0712},
  language     = {eng},
  pages        = {362--368},
  publisher    = {Elsevier},
  series       = {European Journal of Pharmacology},
  title        = {Biochemical and functional correlates of an increased membrane density of caveolae in hypertrophic rat urinary bladder.},
  url          = {http://dx.doi.org/10.1016/j.ejphar.2010.09.050},
  volume       = {649},
  year         = {2010},
}