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The effect of PAMAM G6 dendrimers on the structure of lipid vesicles

Akesson, Anna; Bendtsen, Kristian Moss; Beherens, Manja A.; Pedersen, Jan Skov; Alfredsson, Viveka LU and Gomez, Marite Cardenas (2010) In Physical Chemistry Chemical Physics 12(38). p.12267-12272
Abstract
Dendrimers are polymers with unique properties that make them promising in a variety of applications such as potential drug and gene delivery systems. PAMAM dendrimers, in particular, have been widely investigated and are efficiently translocated into the cell. The mechanism of translocation, however, is still unknown. Recently it was proposed that PAMAM dendrimers are able to open holes in lipid bilayers by stealing lipid from the bilayer and forming "dendrisomes''. The present work intends to contribute in the clarification of this question: why are dendrimers able to translocate into the cell? We create simple models for cell membranes by using small lipid vesicles that present a single lipid phase at physiologically relevant... (More)
Dendrimers are polymers with unique properties that make them promising in a variety of applications such as potential drug and gene delivery systems. PAMAM dendrimers, in particular, have been widely investigated and are efficiently translocated into the cell. The mechanism of translocation, however, is still unknown. Recently it was proposed that PAMAM dendrimers are able to open holes in lipid bilayers by stealing lipid from the bilayer and forming "dendrisomes''. The present work intends to contribute in the clarification of this question: why are dendrimers able to translocate into the cell? We create simple models for cell membranes by using small lipid vesicles that present a single lipid phase at physiologically relevant conditions. We then follow the effect that dendrimers have on the structure of the vesicles by using a combination of various techniques: dynamic light scattering, cryo-TEM and small angle X-ray scattering. We discuss our results with respect to the previous findings and reflect on their possible implications for real translocation in living cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Physical Chemistry Chemical Physics
volume
12
issue
38
pages
12267 - 12272
publisher
Royal Society of Chemistry
external identifiers
  • wos:000282103200052
  • scopus:77957113480
ISSN
1463-9084
DOI
10.1039/c0cp00172d
language
English
LU publication?
yes
id
9d84dc17-b035-4b74-b666-20f65ab05977 (old id 1695862)
date added to LUP
2010-10-25 15:09:41
date last changed
2018-07-15 03:41:28
@article{9d84dc17-b035-4b74-b666-20f65ab05977,
  abstract     = {Dendrimers are polymers with unique properties that make them promising in a variety of applications such as potential drug and gene delivery systems. PAMAM dendrimers, in particular, have been widely investigated and are efficiently translocated into the cell. The mechanism of translocation, however, is still unknown. Recently it was proposed that PAMAM dendrimers are able to open holes in lipid bilayers by stealing lipid from the bilayer and forming "dendrisomes''. The present work intends to contribute in the clarification of this question: why are dendrimers able to translocate into the cell? We create simple models for cell membranes by using small lipid vesicles that present a single lipid phase at physiologically relevant conditions. We then follow the effect that dendrimers have on the structure of the vesicles by using a combination of various techniques: dynamic light scattering, cryo-TEM and small angle X-ray scattering. We discuss our results with respect to the previous findings and reflect on their possible implications for real translocation in living cells.},
  author       = {Akesson, Anna and Bendtsen, Kristian Moss and Beherens, Manja A. and Pedersen, Jan Skov and Alfredsson, Viveka and Gomez, Marite Cardenas},
  issn         = {1463-9084},
  language     = {eng},
  number       = {38},
  pages        = {12267--12272},
  publisher    = {Royal Society of Chemistry},
  series       = {Physical Chemistry Chemical Physics},
  title        = {The effect of PAMAM G6 dendrimers on the structure of lipid vesicles},
  url          = {http://dx.doi.org/10.1039/c0cp00172d},
  volume       = {12},
  year         = {2010},
}