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Retinal transplantation using surface modified poly(glycerol-co-sebacic acid) membranes

Pritchard, Christopher D.; Arnér, Karin LU ; Langer, Robert S. and Ghosh, Fredrik LU (2010) In Biomaterials 31(31). p.7978-7984
Abstract
In retinal transplantation experiments it is hypothesized that remaining diseased photoreceptor cells in the host retina and inner retinal cells in transplants physically obstruct the development of graft-host neuronal contacts which are required for vision. Recently, we developed methods for the isolation of donor photoreceptor layers in vitro, and the selective removal of host photoreceptors in vivo using biodegradable elastomeric membranes composed of poly(glycerol-co-sebacic acid) (PGS). We also coated PGS membranes with electrospun nanofibers, composed of laminin and poly(epsilon-caprolactone) (PCL), to promote attachment of embryonic retinal explants, allowing the resulting composites to be handled surgically as a single entity.... (More)
In retinal transplantation experiments it is hypothesized that remaining diseased photoreceptor cells in the host retina and inner retinal cells in transplants physically obstruct the development of graft-host neuronal contacts which are required for vision. Recently, we developed methods for the isolation of donor photoreceptor layers in vitro, and the selective removal of host photoreceptors in vivo using biodegradable elastomeric membranes composed of poly(glycerol-co-sebacic acid) (PGS). We also coated PGS membranes with electrospun nanofibers, composed of laminin and poly(epsilon-caprolactone) (PCL), to promote attachment of embryonic retinal explants, allowing the resulting composites to be handled surgically as a single entity. Here, we report subretinal transplantation of these composites into adult porcine eyes. In hematoxylin and eosin stained sections of composite explants after 5-7 days in vitro, excellent fusion of retinas and biomaterial membranes was noted, with the immature retinal components showing laminated as well as folded and rosetted areas. The composite grafts could be transplanted in all cases and, 3 months after surgery, eyes displayed clear media, attached retinas and the grafts located subretinally. Histological examination revealed that the biomaterial membrane had degraded without any signs of inflammation. Transplanted retinas displayed areas of rosettes as well as normal lamination. In most cases inner retinal layers were present in the grafts. Laminated areas displayed well-developed photoreceptors adjacent to an intact host retinal pigment epithelium and degeneration of the host outer nuclear layer (ONL) was often observed together with occasional fusion of graft and host inner layers. (c) 2010 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Polycaprolactone, Nanotopography, Cell adhesion, Elastomer, Retina, Transplantation
in
Biomaterials
volume
31
issue
31
pages
7978 - 7984
publisher
Elsevier
external identifiers
  • wos:000282109100012
  • scopus:77956010492
ISSN
1878-5905
DOI
10.1016/j.biomaterials.2010.07.026
language
English
LU publication?
yes
id
a4e7389a-7aa5-4295-9107-7a5cf926fdac (old id 1696044)
date added to LUP
2010-10-25 12:43:50
date last changed
2018-07-01 03:23:41
@article{a4e7389a-7aa5-4295-9107-7a5cf926fdac,
  abstract     = {In retinal transplantation experiments it is hypothesized that remaining diseased photoreceptor cells in the host retina and inner retinal cells in transplants physically obstruct the development of graft-host neuronal contacts which are required for vision. Recently, we developed methods for the isolation of donor photoreceptor layers in vitro, and the selective removal of host photoreceptors in vivo using biodegradable elastomeric membranes composed of poly(glycerol-co-sebacic acid) (PGS). We also coated PGS membranes with electrospun nanofibers, composed of laminin and poly(epsilon-caprolactone) (PCL), to promote attachment of embryonic retinal explants, allowing the resulting composites to be handled surgically as a single entity. Here, we report subretinal transplantation of these composites into adult porcine eyes. In hematoxylin and eosin stained sections of composite explants after 5-7 days in vitro, excellent fusion of retinas and biomaterial membranes was noted, with the immature retinal components showing laminated as well as folded and rosetted areas. The composite grafts could be transplanted in all cases and, 3 months after surgery, eyes displayed clear media, attached retinas and the grafts located subretinally. Histological examination revealed that the biomaterial membrane had degraded without any signs of inflammation. Transplanted retinas displayed areas of rosettes as well as normal lamination. In most cases inner retinal layers were present in the grafts. Laminated areas displayed well-developed photoreceptors adjacent to an intact host retinal pigment epithelium and degeneration of the host outer nuclear layer (ONL) was often observed together with occasional fusion of graft and host inner layers. (c) 2010 Elsevier Ltd. All rights reserved.},
  author       = {Pritchard, Christopher D. and Arnér, Karin and Langer, Robert S. and Ghosh, Fredrik},
  issn         = {1878-5905},
  keyword      = {Polycaprolactone,Nanotopography,Cell adhesion,Elastomer,Retina,Transplantation},
  language     = {eng},
  number       = {31},
  pages        = {7978--7984},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {Retinal transplantation using surface modified poly(glycerol-co-sebacic acid) membranes},
  url          = {http://dx.doi.org/10.1016/j.biomaterials.2010.07.026},
  volume       = {31},
  year         = {2010},
}