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The amphetamine induced rotation test : A re-assessment of its use as a tool to monitor motor impairment and functional recovery in rodent models of Parkinson's disease

Björklund, Anders LU and Dunnett, Stephen B. (2019) In Journal of Parkinson's Disease 9(1). p.17-29
Abstract

Rats and mice with unilateral damage to the nigrostriatal dopamine system-induced by neurotoxins, such as 6-hydroxydopamine, overexpression of α-synuclein, or injections of toxic synuclein protofibrils-are widely used as experimental models to mimic the loss of dopamine neurons seen in Parkinson's disease. The amphetamine rotation test is commonly used to monitor the extent of motor impairment induced by the lesion, and this test has also become the standard tool to demonstrate transplant-induced functional recovery or the efficacy of neuroprotective interventions aimed to preserve or restore DA neuron function. Although the amphetamine-induced rotation test is highly useful for this purpose it has some important pitfalls and the... (More)

Rats and mice with unilateral damage to the nigrostriatal dopamine system-induced by neurotoxins, such as 6-hydroxydopamine, overexpression of α-synuclein, or injections of toxic synuclein protofibrils-are widely used as experimental models to mimic the loss of dopamine neurons seen in Parkinson's disease. The amphetamine rotation test is commonly used to monitor the extent of motor impairment induced by the lesion, and this test has also become the standard tool to demonstrate transplant-induced functional recovery or the efficacy of neuroprotective interventions aimed to preserve or restore DA neuron function. Although the amphetamine-induced rotation test is highly useful for this purpose it has some important pitfalls and the interpretation of the data may not always be straightforward. Unless the test is applied properly and the data are displayed and interpreted appropriately the conclusions may be misleading or simply totally wrong. The purpose of this review is to draw attention to the potential problems and pitfalls involved in the use of drug-induced rotation tests, and to provide recommendations and advice on how to avoid them.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
6-hydroxydopamine, dopamine, Motor behavior, mouse, nigrostriatal system, rat, rotation, α-synuclein
in
Journal of Parkinson's Disease
volume
9
issue
1
pages
13 pages
publisher
IOS Press
external identifiers
  • scopus:85061208805
ISSN
1877-7171
DOI
10.3233/JPD-181525
language
English
LU publication?
yes
id
16b81a3e-e507-4709-9555-b7c80fb18e9f
date added to LUP
2019-02-20 10:59:36
date last changed
2019-03-12 04:22:20
@article{16b81a3e-e507-4709-9555-b7c80fb18e9f,
  abstract     = {<p>Rats and mice with unilateral damage to the nigrostriatal dopamine system-induced by neurotoxins, such as 6-hydroxydopamine, overexpression of α-synuclein, or injections of toxic synuclein protofibrils-are widely used as experimental models to mimic the loss of dopamine neurons seen in Parkinson's disease. The amphetamine rotation test is commonly used to monitor the extent of motor impairment induced by the lesion, and this test has also become the standard tool to demonstrate transplant-induced functional recovery or the efficacy of neuroprotective interventions aimed to preserve or restore DA neuron function. Although the amphetamine-induced rotation test is highly useful for this purpose it has some important pitfalls and the interpretation of the data may not always be straightforward. Unless the test is applied properly and the data are displayed and interpreted appropriately the conclusions may be misleading or simply totally wrong. The purpose of this review is to draw attention to the potential problems and pitfalls involved in the use of drug-induced rotation tests, and to provide recommendations and advice on how to avoid them.</p>},
  author       = {Björklund, Anders and Dunnett, Stephen B.},
  issn         = {1877-7171},
  keyword      = {6-hydroxydopamine,dopamine,Motor behavior,mouse,nigrostriatal system,rat,rotation,α-synuclein},
  language     = {eng},
  number       = {1},
  pages        = {17--29},
  publisher    = {IOS Press},
  series       = {Journal of Parkinson's Disease},
  title        = {The amphetamine induced rotation test : A re-assessment of its use as a tool to monitor motor impairment and functional recovery in rodent models of Parkinson's disease},
  url          = {http://dx.doi.org/10.3233/JPD-181525},
  volume       = {9},
  year         = {2019},
}