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Induction of precocious intestinal maturation in T-cell deficient athymic neonatal rats

Sureda, Ester Arévalo LU ; Gidlund, Catherine; Weström, Björn LU and Prykhodko, Olena LU (2017) In World Journal of Gastroenterology 23(42). p.7531-7540
Abstract

Aim: To investigate whether gut maturation could be induced precociously in an athymic T-cell deficient neonatal rat model. Methods: Fourteen day-old athymic (nude) rats (NIH-Foxn 1rnu) were gavaged with either phytohaemagglutinin - a lectin from red kidney beans (PHA); trypsin - a protease (Prot); or water - vehicle (control) as a single dose on one day or once a day for 3-day. The nude rats were either nurtured by their mothers or cross-fostered by conventional foster dams of the Sprague-Dawley strain from days 3-5 after birth. At 17 d of age, 72 h after administration of the first treatment, intestinal macromolecular permeability was tested in vivo, prior to euthanasia, after which blood and gut organs were sampled.... (More)

Aim: To investigate whether gut maturation could be induced precociously in an athymic T-cell deficient neonatal rat model. Methods: Fourteen day-old athymic (nude) rats (NIH-Foxn 1rnu) were gavaged with either phytohaemagglutinin - a lectin from red kidney beans (PHA); trypsin - a protease (Prot); or water - vehicle (control) as a single dose on one day or once a day for 3-day. The nude rats were either nurtured by their mothers or cross-fostered by conventional foster dams of the Sprague-Dawley strain from days 3-5 after birth. At 17 d of age, 72 h after administration of the first treatment, intestinal macromolecular permeability was tested in vivo, prior to euthanasia, after which blood and gut organs were sampled. Results: Provocation with both, PHA and protease, resulted in increased gut growth and maturation in nude rat pups independent of nursing. Foetal-type enterocytes were replaced by non-vacuolated adult-type enterocytes in the distal small intestine epithelium. Decreased intestinal macromolecular permeability (gut closure) was observed, with reduced permeability markers (BIgG and BSA, P < 0.001) in circulation. Increased pancreatic function, with an increased trypsin to protein ratio in pancreas homogenates, was observed independent of nursing in the nude pups. Immunostaining showed the presence of a few CD3+-cells in the intestinal mucosa of the nude pups. The number of CD3+-cells remained unaltered by provocation and no differences were observed between the nursing sets. Growth and vitality of the nude pups were dependent on nurturing, since cross-fostering by conventional dams increased their macromolecular absorptive capacity (BSA, P < 0.05), as well as their passive immunity (RIgG, P < 0.05). Conclusion: Precocious gut maturation can be induced by enteral provocation in athymic rat pups, similarly to in euthymic pups, thus showing an independence from thymusderived T-cells.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Gut, Immunoglobulin G, Intestinal permeability, Pancreas, Passive immunity
in
World Journal of Gastroenterology
volume
23
issue
42
pages
10 pages
publisher
WJG Press
external identifiers
  • scopus:85034108416
  • wos:000414872500004
ISSN
1007-9327
DOI
10.3748/wjg.v23.i42.7531
language
English
LU publication?
yes
id
16c5719d-2e6e-4a26-a0c6-b1494050151e
date added to LUP
2017-12-19 13:13:07
date last changed
2018-03-21 03:00:09
@article{16c5719d-2e6e-4a26-a0c6-b1494050151e,
  abstract     = {<p>Aim: To investigate whether gut maturation could be induced precociously in an athymic T-cell deficient neonatal rat model. Methods: Fourteen day-old athymic (nude) rats (NIH-Foxn 1<sup>rnu</sup>) were gavaged with either phytohaemagglutinin - a lectin from red kidney beans (PHA); trypsin - a protease (Prot); or water - vehicle (control) as a single dose on one day or once a day for 3-day. The nude rats were either nurtured by their mothers or cross-fostered by conventional foster dams of the Sprague-Dawley strain from days 3-5 after birth. At 17 d of age, 72 h after administration of the first treatment, intestinal macromolecular permeability was tested in vivo, prior to euthanasia, after which blood and gut organs were sampled. Results: Provocation with both, PHA and protease, resulted in increased gut growth and maturation in nude rat pups independent of nursing. Foetal-type enterocytes were replaced by non-vacuolated adult-type enterocytes in the distal small intestine epithelium. Decreased intestinal macromolecular permeability (gut closure) was observed, with reduced permeability markers (BIgG and BSA, P &lt; 0.001) in circulation. Increased pancreatic function, with an increased trypsin to protein ratio in pancreas homogenates, was observed independent of nursing in the nude pups. Immunostaining showed the presence of a few CD3<sup>+</sup>-cells in the intestinal mucosa of the nude pups. The number of CD3<sup>+</sup>-cells remained unaltered by provocation and no differences were observed between the nursing sets. Growth and vitality of the nude pups were dependent on nurturing, since cross-fostering by conventional dams increased their macromolecular absorptive capacity (BSA, P &lt; 0.05), as well as their passive immunity (RIgG, P &lt; 0.05). Conclusion: Precocious gut maturation can be induced by enteral provocation in athymic rat pups, similarly to in euthymic pups, thus showing an independence from thymusderived T-cells.</p>},
  author       = {Sureda, Ester Arévalo and Gidlund, Catherine and Weström, Björn and Prykhodko, Olena},
  issn         = {1007-9327},
  keyword      = {Gut,Immunoglobulin G,Intestinal permeability,Pancreas,Passive immunity},
  language     = {eng},
  month        = {11},
  number       = {42},
  pages        = {7531--7540},
  publisher    = {WJG Press},
  series       = {World Journal of Gastroenterology},
  title        = {Induction of precocious intestinal maturation in T-cell deficient athymic neonatal rats},
  url          = {http://dx.doi.org/10.3748/wjg.v23.i42.7531},
  volume       = {23},
  year         = {2017},
}