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The nanoparticle protein corona formed in human blood or human blood fractions

Lundqvist, Martin LU ; Augustsson, Cecilia; Lilja, Malin; Lundkvist, Kristoffer; Dahlbäck, Björn LU ; Linse, Sara LU and Cedervall, Tommy LU (2017) In PLoS ONE 12(4).
Abstract

The protein corona formed around nanoparticles in protein-rich fluids plays an important role for nanoparticle biocompatibility, as found in several studies during the last decade. Biological fluids have complex compositions and the molecular components interact and function together in intricate networks. Therefore, the process to isolate blood or the preparation of blood derivatives may lead to differences in the composition of the identified protein corona around nanoparticles. Here, we show distinct differences in the protein corona formed in whole blood, whole blood with EDTA, plasma, or serum. Furthermore, the ratio between particle surface area to protein concentration influences the detected corona. We also show that the... (More)

The protein corona formed around nanoparticles in protein-rich fluids plays an important role for nanoparticle biocompatibility, as found in several studies during the last decade. Biological fluids have complex compositions and the molecular components interact and function together in intricate networks. Therefore, the process to isolate blood or the preparation of blood derivatives may lead to differences in the composition of the identified protein corona around nanoparticles. Here, we show distinct differences in the protein corona formed in whole blood, whole blood with EDTA, plasma, or serum. Furthermore, the ratio between particle surface area to protein concentration influences the detected corona. We also show that the nanoparticle size per se influences the formed protein corona due to curvature effects. These results emphasize the need of investigating the formation and biological importance of the protein corona in the same environment as the nanoparticles are intended for or released into.

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author
organization
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type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
12
issue
4
publisher
Public Library of Science
external identifiers
  • scopus:85017535670
  • wos:000399874800070
ISSN
1932-6203
DOI
10.1371/journal.pone.0175871
language
English
LU publication?
yes
id
16ce132e-6be5-4a2e-8ad0-da04b755d6a1
date added to LUP
2017-05-23 09:17:18
date last changed
2017-09-18 11:36:40
@article{16ce132e-6be5-4a2e-8ad0-da04b755d6a1,
  abstract     = {<p>The protein corona formed around nanoparticles in protein-rich fluids plays an important role for nanoparticle biocompatibility, as found in several studies during the last decade. Biological fluids have complex compositions and the molecular components interact and function together in intricate networks. Therefore, the process to isolate blood or the preparation of blood derivatives may lead to differences in the composition of the identified protein corona around nanoparticles. Here, we show distinct differences in the protein corona formed in whole blood, whole blood with EDTA, plasma, or serum. Furthermore, the ratio between particle surface area to protein concentration influences the detected corona. We also show that the nanoparticle size per se influences the formed protein corona due to curvature effects. These results emphasize the need of investigating the formation and biological importance of the protein corona in the same environment as the nanoparticles are intended for or released into.</p>},
  articleno    = {e0175871},
  author       = {Lundqvist, Martin and Augustsson, Cecilia and Lilja, Malin and Lundkvist, Kristoffer and Dahlbäck, Björn and Linse, Sara and Cedervall, Tommy},
  issn         = {1932-6203},
  language     = {eng},
  month        = {04},
  number       = {4},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {The nanoparticle protein corona formed in human blood or human blood fractions},
  url          = {http://dx.doi.org/10.1371/journal.pone.0175871},
  volume       = {12},
  year         = {2017},
}