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Assessing lesion malignancy by scanning small-angle x-ray scattering of breast tissue with microcalcifications

Arboleda, C. ; Lutz-Bueno, V. ; Wang, Z. LU ; Villanueva-Perez, P. LU ; Guizar-Sicairos, M. ; Liebi, M. LU ; Varga, Zsuzsanna and Stampanoni, M. (2019) In Physics in Medicine and Biology 64(15).
Abstract

Scanning small-angle x-ray scattering (SAXS) measurements were performed on 36 formalin-fixed reast tissue biopsies obtained from two patients. All samples contained microcalcifications of ype II, i.e. ormed by hydroxyapatite. We demonstrate the feasibility of classifying breast lesions by canning SAXS of issues containing microcalcifications with a resolution of 35 μm x30 μm. We eport a characteristic Bragg eak found around q = 1.725 nm-1 that occurs primarily for malignant esions. Such a clear SAXS fingerprint s potentially linked to structural changes of breast tissue nd corresponds to dimensions of about 3.7 nm. This aterial property could be used as an early ndicator of malignancy development, as it is readily assessed by AXS. If... (More)

Scanning small-angle x-ray scattering (SAXS) measurements were performed on 36 formalin-fixed reast tissue biopsies obtained from two patients. All samples contained microcalcifications of ype II, i.e. ormed by hydroxyapatite. We demonstrate the feasibility of classifying breast lesions by canning SAXS of issues containing microcalcifications with a resolution of 35 μm x30 μm. We eport a characteristic Bragg eak found around q = 1.725 nm-1 that occurs primarily for malignant esions. Such a clear SAXS fingerprint s potentially linked to structural changes of breast tissue nd corresponds to dimensions of about 3.7 nm. This aterial property could be used as an early ndicator of malignancy development, as it is readily assessed by AXS. If this fingerprint is combined ith other known SAXS features, which also indicate the level of alignancy, such as lipid spacing and collagen periodicity, it could complement traditional pathology-based analyses. To confirm the SAXS-based classification, a histopathological workup and a gold standard histopathological diagnosis were conducted to determine the malignancy level of the lesions. Our aim is to report this SAXS fingerprint, which is clearly related to malignant breast lesions. However, any further conclusion based on our dataset is limited by the low number of patients and samples. Running a broad study to increase the number of samples and patients is of great importance and relevance for the breast-imaging community.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
breast cancer, breast lesion diagnosis, breast micro calcifications, small-angle x-ray scattering
in
Physics in Medicine and Biology
volume
64
issue
15
article number
155010
pages
9 pages
publisher
IOP Publishing
external identifiers
  • scopus:85071322821
  • pmid:31234149
ISSN
0031-9155
DOI
10.1088/1361-6560/ab2c36
language
English
LU publication?
yes
id
16e22f48-65b3-4a5b-b1e1-184f8645bf45
date added to LUP
2019-10-11 16:23:02
date last changed
2019-10-13 02:59:11
@article{16e22f48-65b3-4a5b-b1e1-184f8645bf45,
  abstract     = {<p>Scanning small-angle x-ray scattering (SAXS) measurements were performed on 36 formalin-fixed reast tissue biopsies obtained from two patients. All samples contained microcalcifications of ype II, i.e. ormed by hydroxyapatite. We demonstrate the feasibility of classifying breast lesions by canning SAXS of issues containing microcalcifications with a resolution of 35 μm x30 μm. We eport a characteristic Bragg eak found around q = 1.725 nm-1 that occurs primarily for malignant esions. Such a clear SAXS fingerprint s potentially linked to structural changes of breast tissue nd corresponds to dimensions of about 3.7 nm. This aterial property could be used as an early ndicator of malignancy development, as it is readily assessed by AXS. If this fingerprint is combined ith other known SAXS features, which also indicate the level of alignancy, such as lipid spacing and collagen periodicity, it could complement traditional pathology-based analyses. To confirm the SAXS-based classification, a histopathological workup and a gold standard histopathological diagnosis were conducted to determine the malignancy level of the lesions. Our aim is to report this SAXS fingerprint, which is clearly related to malignant breast lesions. However, any further conclusion based on our dataset is limited by the low number of patients and samples. Running a broad study to increase the number of samples and patients is of great importance and relevance for the breast-imaging community.</p>},
  author       = {Arboleda, C. and Lutz-Bueno, V. and Wang, Z. and Villanueva-Perez, P. and Guizar-Sicairos, M. and Liebi, M. and Varga, Zsuzsanna and Stampanoni, M.},
  issn         = {0031-9155},
  language     = {eng},
  month        = {08},
  number       = {15},
  publisher    = {IOP Publishing},
  series       = {Physics in Medicine and Biology},
  title        = {Assessing lesion malignancy by scanning small-angle x-ray scattering of breast tissue with microcalcifications},
  url          = {http://dx.doi.org/10.1088/1361-6560/ab2c36},
  doi          = {10.1088/1361-6560/ab2c36},
  volume       = {64},
  year         = {2019},
}