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The ms2io6A37 modification of tRNA in Salmonella typhimurium regulates growth on citric acid cycle intermediates

Persson, Britt C.; Olafsson, Olafur; Lundgren, Hans K. and Hederstedt, Lars LU (1998) In Journal of Bacteriology 180(12). p.3144-3151
Abstract
The modified nucleoside 2-methylthio-N-6-isopentenyl adenosine (ms(2)i(6)A) is present in position 37 (adjacent to and 3' of the anticodon) of tRNAs that read codons beginning with U except tRNA(I,V)(Ser) in Escherichia coli, In Salmonella typhimurium , 2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine (ms(2)io(6)A; also referred to as 2-methylthio cis-ribozeatin) is found in tRNA, most likely in the species that have ms(2)i(6)A in E. coli. Mutants (miaE) of S. typhimurium in which ms(2)i(6)A hydroxylation is blocked are unable to grow aerobically on the dicarboxylic acids of the citric acid cycle. Such mutants have normal uptake of dicarboxylic acids and functional enzymes of the citric acid cycle and the aerobic respiratory chain. The... (More)
The modified nucleoside 2-methylthio-N-6-isopentenyl adenosine (ms(2)i(6)A) is present in position 37 (adjacent to and 3' of the anticodon) of tRNAs that read codons beginning with U except tRNA(I,V)(Ser) in Escherichia coli, In Salmonella typhimurium , 2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine (ms(2)io(6)A; also referred to as 2-methylthio cis-ribozeatin) is found in tRNA, most likely in the species that have ms(2)i(6)A in E. coli. Mutants (miaE) of S. typhimurium in which ms(2)i(6)A hydroxylation is blocked are unable to grow aerobically on the dicarboxylic acids of the citric acid cycle. Such mutants have normal uptake of dicarboxylic acids and functional enzymes of the citric acid cycle and the aerobic respiratory chain. The ability of S. typhimurium Bo grow ore succinate, fumarate, and malate is dependent on the state of modification in position 37 of those tRNAs normally having ms(2)io(6)A37 and is not due to a second cellular function of tRNA (ms(2)io(6)A37)hydroxylase, the miaE gene product. We suggest that S. typhimurium senses the hydroxylation status of the isopentenyl group of the tRNA and will grow on succinate, fumarate, or malate only if the isopentenyl group is hydroxylated. (Less)
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Contribution to journal
publication status
published
subject
in
Journal of Bacteriology
volume
180
issue
12
pages
3144 - 3151
publisher
American Society for Microbiology
ISSN
0021-9193
language
English
LU publication?
yes
id
17018f97-54eb-4f2d-bbb9-07dbe1fb6e8f
date added to LUP
2017-07-18 09:54:02
date last changed
2017-08-11 12:25:22
@article{17018f97-54eb-4f2d-bbb9-07dbe1fb6e8f,
  abstract     = {The modified nucleoside 2-methylthio-N-6-isopentenyl adenosine (ms(2)i(6)A) is present in position 37 (adjacent to and 3' of the anticodon) of tRNAs that read codons beginning with U except tRNA(I,V)(Ser) in Escherichia coli, In Salmonella typhimurium , 2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine (ms(2)io(6)A; also referred to as 2-methylthio cis-ribozeatin) is found in tRNA, most likely in the species that have ms(2)i(6)A in E. coli. Mutants (miaE) of S. typhimurium in which ms(2)i(6)A hydroxylation is blocked are unable to grow aerobically on the dicarboxylic acids of the citric acid cycle. Such mutants have normal uptake of dicarboxylic acids and functional enzymes of the citric acid cycle and the aerobic respiratory chain. The ability of S. typhimurium Bo grow ore succinate, fumarate, and malate is dependent on the state of modification in position 37 of those tRNAs normally having ms(2)io(6)A37 and is not due to a second cellular function of tRNA (ms(2)io(6)A37)hydroxylase, the miaE gene product. We suggest that S. typhimurium senses the hydroxylation status of the isopentenyl group of the tRNA and will grow on succinate, fumarate, or malate only if the isopentenyl group is hydroxylated.},
  author       = {Persson, Britt C. and Olafsson, Olafur and Lundgren, Hans K. and Hederstedt, Lars},
  issn         = {0021-9193},
  language     = {eng},
  number       = {12},
  pages        = {3144--3151},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Bacteriology},
  title        = {The ms2io6A37 modification of tRNA in <em>Salmonella typhimurium</em> regulates growth on citric acid cycle intermediates},
  volume       = {180},
  year         = {1998},
}