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DNA and RNA binding by the Wilms' tumour gene 1 (WT1) protein +KTS and −KTS isoforms—From initial observations to recent global genomic analyses

Ullmark, Tove LU ; Montano, Giorgia LU and Gullberg, Urban LU (2018) In European Journal of Haematology 100(3). p.229-240
Abstract

The Wilms' tumour gene 1 protein (WT1) is a zinc finger transcription factor found indispensable for foetal development. WT1 has also been implicated in the development of tumours in several organ systems, including acute myeloid leukaemia (AML). Four main WT1 isoforms come from 2 alternative splice events. One alternative splice results in the inclusion or exclusion of 3 amino acids, KTS, between zinc fingers 3 and 4 in the WT1 protein. The KTS insert has been extensively investigated due to the functional implications for DNA and RNA binding. In this review, we provide an overview of the research into the isoforms containing or lacking the KTS insert in leukaemic cells, as well as the research into the binding patterns of the WT1 −KTS... (More)

The Wilms' tumour gene 1 protein (WT1) is a zinc finger transcription factor found indispensable for foetal development. WT1 has also been implicated in the development of tumours in several organ systems, including acute myeloid leukaemia (AML). Four main WT1 isoforms come from 2 alternative splice events. One alternative splice results in the inclusion or exclusion of 3 amino acids, KTS, between zinc fingers 3 and 4 in the WT1 protein. The KTS insert has been extensively investigated due to the functional implications for DNA and RNA binding. In this review, we provide an overview of the research into the isoforms containing or lacking the KTS insert in leukaemic cells, as well as the research into the binding patterns of the WT1 −KTS and +KTS isoforms to DNA and RNA. Finally, we connect the results of the DNA binding research to the ChIP-CHIP and ChIP-Seq investigations into the global genomic binding of the WT1 protein that have recently been performed.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
acute lymphoblastic leukemia, acute myeloid leukemia, hematopoiesis and hematopathology, molecular biology of myelopoiesis
in
European Journal of Haematology
volume
100
issue
3
pages
12 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:85040524034
ISSN
0902-4441
DOI
10.1111/ejh.13010
language
English
LU publication?
yes
id
170470cc-ccfd-427c-bc7f-c0dd9caf329f
date added to LUP
2018-02-23 19:04:07
date last changed
2018-05-29 11:28:58
@article{170470cc-ccfd-427c-bc7f-c0dd9caf329f,
  abstract     = {<p>The Wilms' tumour gene 1 protein (WT1) is a zinc finger transcription factor found indispensable for foetal development. WT1 has also been implicated in the development of tumours in several organ systems, including acute myeloid leukaemia (AML). Four main WT1 isoforms come from 2 alternative splice events. One alternative splice results in the inclusion or exclusion of 3 amino acids, KTS, between zinc fingers 3 and 4 in the WT1 protein. The KTS insert has been extensively investigated due to the functional implications for DNA and RNA binding. In this review, we provide an overview of the research into the isoforms containing or lacking the KTS insert in leukaemic cells, as well as the research into the binding patterns of the WT1 −KTS and +KTS isoforms to DNA and RNA. Finally, we connect the results of the DNA binding research to the ChIP-CHIP and ChIP-Seq investigations into the global genomic binding of the WT1 protein that have recently been performed.</p>},
  author       = {Ullmark, Tove and Montano, Giorgia and Gullberg, Urban},
  issn         = {0902-4441},
  keyword      = {acute lymphoblastic leukemia,acute myeloid leukemia,hematopoiesis and hematopathology,molecular biology of myelopoiesis},
  language     = {eng},
  month        = {03},
  number       = {3},
  pages        = {229--240},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Haematology},
  title        = {DNA and RNA binding by the Wilms' tumour gene 1 (WT1) protein +KTS and −KTS isoforms—From initial observations to recent global genomic analyses},
  url          = {http://dx.doi.org/10.1111/ejh.13010},
  volume       = {100},
  year         = {2018},
}