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Extraction, quantitation, and evaluation of function DNA from various sample types.

Ivarsson, Malin and Carlson, Joyce LU (2011) In Methods in Molecular Biology 675. p.261-277
Abstract
Two vital pre-requisites for genetic epidemiology have been fullfiled during the past decade and have led to a virtual explosion of knowledge concerning disease risks. Reliable databases over genetic variation derived from, e.g. the HUGO and HapMap projects, coupled with technological advances make large-scale genetic analyses and downstream bioinformatics suddenly affordable. Although recent prospective population-based biobanks have included DNA collection and purification in their planning, it is the older projects that currently are of greatest value due to the numbers of accumulated disease endpoints. In this chapter, methods to purify and use DNA derived from a variety of archival materials, including whole blood, formalin-fixed... (More)
Two vital pre-requisites for genetic epidemiology have been fullfiled during the past decade and have led to a virtual explosion of knowledge concerning disease risks. Reliable databases over genetic variation derived from, e.g. the HUGO and HapMap projects, coupled with technological advances make large-scale genetic analyses and downstream bioinformatics suddenly affordable. Although recent prospective population-based biobanks have included DNA collection and purification in their planning, it is the older projects that currently are of greatest value due to the numbers of accumulated disease endpoints. In this chapter, methods to purify and use DNA derived from a variety of archival materials, including whole blood, formalin-fixed paraffin-embedded (FFPE) tissues, sera, dried blood spots (DBS), cervical cell suspensions, and mouthwash are presented and evaluated in a context of quality control guidelines to provide objective measure of the usefulness of various sample types for genetic epidemiology. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
675
pages
261 - 277
publisher
Springer
external identifiers
  • pmid:20949395
  • scopus:79952115859
ISSN
1940-6029
DOI
10.1007/978-1-59745-423-0_14
language
English
LU publication?
yes
id
b7297293-3026-43c0-9041-c423746d8a66 (old id 1711120)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20949395?dopt=Abstract
date added to LUP
2010-11-05 09:45:32
date last changed
2017-01-01 03:03:21
@article{b7297293-3026-43c0-9041-c423746d8a66,
  abstract     = {Two vital pre-requisites for genetic epidemiology have been fullfiled during the past decade and have led to a virtual explosion of knowledge concerning disease risks. Reliable databases over genetic variation derived from, e.g. the HUGO and HapMap projects, coupled with technological advances make large-scale genetic analyses and downstream bioinformatics suddenly affordable. Although recent prospective population-based biobanks have included DNA collection and purification in their planning, it is the older projects that currently are of greatest value due to the numbers of accumulated disease endpoints. In this chapter, methods to purify and use DNA derived from a variety of archival materials, including whole blood, formalin-fixed paraffin-embedded (FFPE) tissues, sera, dried blood spots (DBS), cervical cell suspensions, and mouthwash are presented and evaluated in a context of quality control guidelines to provide objective measure of the usefulness of various sample types for genetic epidemiology.},
  author       = {Ivarsson, Malin and Carlson, Joyce},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {261--277},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Extraction, quantitation, and evaluation of function DNA from various sample types.},
  url          = {http://dx.doi.org/10.1007/978-1-59745-423-0_14},
  volume       = {675},
  year         = {2011},
}