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Regulation of selenoprotein mRNA expression by hormones and retinoic acid in bovine mammary cells.

Bruzelius, Katharina; Sundler, Roger LU ; Pagmantidis, Vasileios and Åkesson, Björn (2010) In Journal of Trace Elements in Medicine and Biology 24. p.251-256
Abstract
Selenium is essential for maintaining many body functions through the actions of selenoproteins. To find factors regulating selenoprotein biosynthesis in the bovine mammary cell line MAC-T, the effects of supplementation with selenite and also with retinoic acid, insulin, hydrocortisone and prolactin on the mRNA expression of a number of selenoproteins were investigated. It was found that MAC-T cells express glutathione peroxidase (GPx) 1 and 4, thioredoxin reductase 1 and selenoprotein P, but not GPx 3, which is interesting considering that GPx 3 is one of the only few selenoproteins detected in milk so far. Addition of selenite to the cell culture resulted in a large increase in GPx 1 expression and an increase in selenoprotein P... (More)
Selenium is essential for maintaining many body functions through the actions of selenoproteins. To find factors regulating selenoprotein biosynthesis in the bovine mammary cell line MAC-T, the effects of supplementation with selenite and also with retinoic acid, insulin, hydrocortisone and prolactin on the mRNA expression of a number of selenoproteins were investigated. It was found that MAC-T cells express glutathione peroxidase (GPx) 1 and 4, thioredoxin reductase 1 and selenoprotein P, but not GPx 3, which is interesting considering that GPx 3 is one of the only few selenoproteins detected in milk so far. Addition of selenite to the cell culture resulted in a large increase in GPx 1 expression and an increase in selenoprotein P expression, which is similar to the findings made in other systems investigated. Increased mRNA levels of GPx 1 were also observed in cells treated with insulin and hydrocortisone or with retinoic acid. The expression of thioredoxin reductase 1 was increased in cells treated with retinoic acid, whereas that of selenoprotein P was decreased in cells exposed to insulin. The results indicate that several hormones, selenium, and retinoic acid regulate the biosynthesis of various selenoproteins differently in the bovine mammary cell. The possible implications of the findings for processes related to milk formation and mammary carcinogenesis will need additional investigation. Further study of the detailed mechanisms involved is also necessary. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Trace Elements in Medicine and Biology
volume
24
pages
251 - 256
publisher
Elsevier
external identifiers
  • wos:000286018200005
  • pmid:20943360
  • scopus:78549238501
ISSN
1878-3252
DOI
10.1016/j.jtemb.2010.08.001
language
English
LU publication?
yes
id
13c84503-5779-476b-a269-882461f3493d (old id 1711215)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20943360?dopt=Abstract
date added to LUP
2010-11-05 15:50:35
date last changed
2018-06-17 04:55:10
@article{13c84503-5779-476b-a269-882461f3493d,
  abstract     = {Selenium is essential for maintaining many body functions through the actions of selenoproteins. To find factors regulating selenoprotein biosynthesis in the bovine mammary cell line MAC-T, the effects of supplementation with selenite and also with retinoic acid, insulin, hydrocortisone and prolactin on the mRNA expression of a number of selenoproteins were investigated. It was found that MAC-T cells express glutathione peroxidase (GPx) 1 and 4, thioredoxin reductase 1 and selenoprotein P, but not GPx 3, which is interesting considering that GPx 3 is one of the only few selenoproteins detected in milk so far. Addition of selenite to the cell culture resulted in a large increase in GPx 1 expression and an increase in selenoprotein P expression, which is similar to the findings made in other systems investigated. Increased mRNA levels of GPx 1 were also observed in cells treated with insulin and hydrocortisone or with retinoic acid. The expression of thioredoxin reductase 1 was increased in cells treated with retinoic acid, whereas that of selenoprotein P was decreased in cells exposed to insulin. The results indicate that several hormones, selenium, and retinoic acid regulate the biosynthesis of various selenoproteins differently in the bovine mammary cell. The possible implications of the findings for processes related to milk formation and mammary carcinogenesis will need additional investigation. Further study of the detailed mechanisms involved is also necessary.},
  author       = {Bruzelius, Katharina and Sundler, Roger and Pagmantidis, Vasileios and Åkesson, Björn},
  issn         = {1878-3252},
  language     = {eng},
  pages        = {251--256},
  publisher    = {Elsevier},
  series       = {Journal of Trace Elements in Medicine and Biology},
  title        = {Regulation of selenoprotein mRNA expression by hormones and retinoic acid in bovine mammary cells.},
  url          = {http://dx.doi.org/10.1016/j.jtemb.2010.08.001},
  volume       = {24},
  year         = {2010},
}