Regulation of selenoprotein mRNA expression by hormones and retinoic acid in bovine mammary cells.
(2010) In Journal of Trace Elements in Medicine and Biology 24. p.251-256- Abstract
- Selenium is essential for maintaining many body functions through the actions of selenoproteins. To find factors regulating selenoprotein biosynthesis in the bovine mammary cell line MAC-T, the effects of supplementation with selenite and also with retinoic acid, insulin, hydrocortisone and prolactin on the mRNA expression of a number of selenoproteins were investigated. It was found that MAC-T cells express glutathione peroxidase (GPx) 1 and 4, thioredoxin reductase 1 and selenoprotein P, but not GPx 3, which is interesting considering that GPx 3 is one of the only few selenoproteins detected in milk so far. Addition of selenite to the cell culture resulted in a large increase in GPx 1 expression and an increase in selenoprotein P... (More)
- Selenium is essential for maintaining many body functions through the actions of selenoproteins. To find factors regulating selenoprotein biosynthesis in the bovine mammary cell line MAC-T, the effects of supplementation with selenite and also with retinoic acid, insulin, hydrocortisone and prolactin on the mRNA expression of a number of selenoproteins were investigated. It was found that MAC-T cells express glutathione peroxidase (GPx) 1 and 4, thioredoxin reductase 1 and selenoprotein P, but not GPx 3, which is interesting considering that GPx 3 is one of the only few selenoproteins detected in milk so far. Addition of selenite to the cell culture resulted in a large increase in GPx 1 expression and an increase in selenoprotein P expression, which is similar to the findings made in other systems investigated. Increased mRNA levels of GPx 1 were also observed in cells treated with insulin and hydrocortisone or with retinoic acid. The expression of thioredoxin reductase 1 was increased in cells treated with retinoic acid, whereas that of selenoprotein P was decreased in cells exposed to insulin. The results indicate that several hormones, selenium, and retinoic acid regulate the biosynthesis of various selenoproteins differently in the bovine mammary cell. The possible implications of the findings for processes related to milk formation and mammary carcinogenesis will need additional investigation. Further study of the detailed mechanisms involved is also necessary. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1711215
- author
- Bruzelius, Katharina LU ; Sundler, Roger LU ; Pagmantidis, Vasileios and Åkesson, Björn LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Trace Elements in Medicine and Biology
- volume
- 24
- pages
- 251 - 256
- publisher
- Elsevier
- external identifiers
-
- wos:000286018200005
- pmid:20943360
- scopus:78549238501
- ISSN
- 1878-3252
- DOI
- 10.1016/j.jtemb.2010.08.001
- language
- English
- LU publication?
- yes
- id
- 13c84503-5779-476b-a269-882461f3493d (old id 1711215)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20943360?dopt=Abstract
- date added to LUP
- 2016-04-04 07:12:38
- date last changed
- 2022-01-29 01:54:58
@article{13c84503-5779-476b-a269-882461f3493d,
abstract = {{Selenium is essential for maintaining many body functions through the actions of selenoproteins. To find factors regulating selenoprotein biosynthesis in the bovine mammary cell line MAC-T, the effects of supplementation with selenite and also with retinoic acid, insulin, hydrocortisone and prolactin on the mRNA expression of a number of selenoproteins were investigated. It was found that MAC-T cells express glutathione peroxidase (GPx) 1 and 4, thioredoxin reductase 1 and selenoprotein P, but not GPx 3, which is interesting considering that GPx 3 is one of the only few selenoproteins detected in milk so far. Addition of selenite to the cell culture resulted in a large increase in GPx 1 expression and an increase in selenoprotein P expression, which is similar to the findings made in other systems investigated. Increased mRNA levels of GPx 1 were also observed in cells treated with insulin and hydrocortisone or with retinoic acid. The expression of thioredoxin reductase 1 was increased in cells treated with retinoic acid, whereas that of selenoprotein P was decreased in cells exposed to insulin. The results indicate that several hormones, selenium, and retinoic acid regulate the biosynthesis of various selenoproteins differently in the bovine mammary cell. The possible implications of the findings for processes related to milk formation and mammary carcinogenesis will need additional investigation. Further study of the detailed mechanisms involved is also necessary.}},
author = {{Bruzelius, Katharina and Sundler, Roger and Pagmantidis, Vasileios and Åkesson, Björn}},
issn = {{1878-3252}},
language = {{eng}},
pages = {{251--256}},
publisher = {{Elsevier}},
series = {{Journal of Trace Elements in Medicine and Biology}},
title = {{Regulation of selenoprotein mRNA expression by hormones and retinoic acid in bovine mammary cells.}},
url = {{https://lup.lub.lu.se/search/files/5130136/1737728.pdf}},
doi = {{10.1016/j.jtemb.2010.08.001}},
volume = {{24}},
year = {{2010}},
}