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A simple and sensitive method for lactose detection based on direct electron transfer between immobilised cellobiose dehydrogenase and screen-printed carbon electrodes

Safina, Gulnara LU ; Ludwig, Roland and Gorton, Lo LU (2010) 7th Spring Meeting of the International-Society-of-Electrochemistry on Recent Advances in Electrocatalysis and Photoelectrocatalysis In Electrochimica Acta 55(26). p.7690-7695
Abstract
A rapid and simple approach of lactose analysis is proposed based on 3rd generation amperometric biosensors employing cellobiose dehydrogenase (CDH) from Trametes villosa or Phanerochaete sordida immobilised on screen-printed carbon electrodes (SPCEs). After optimisation of the working conditions of the biosensors - pH of the carrier buffer, flow rate and applied potential - the sensors were able to detect lactose in a concentration range between 0.5-200 mu M and 0.5-100 mu M employing T. villosa and P. sordida CDH, respectively. The limit of detection is 250 nM (90 mu g/L) for both. Biosensors based on SPCEs modified with multiwalled carbon nanotubes showed a higher sensitivity than unmodified SPCEs. Crosslinking with glutaraldehyde or... (More)
A rapid and simple approach of lactose analysis is proposed based on 3rd generation amperometric biosensors employing cellobiose dehydrogenase (CDH) from Trametes villosa or Phanerochaete sordida immobilised on screen-printed carbon electrodes (SPCEs). After optimisation of the working conditions of the biosensors - pH of the carrier buffer, flow rate and applied potential - the sensors were able to detect lactose in a concentration range between 0.5-200 mu M and 0.5-100 mu M employing T. villosa and P. sordida CDH, respectively. The limit of detection is 250 nM (90 mu g/L) for both. Biosensors based on SPCEs modified with multiwalled carbon nanotubes showed a higher sensitivity than unmodified SPCEs. Crosslinking with glutaraldehyde or poly(ethyleneglycol)diglycidyl ether improved not only the stability but also the analytical response. The developed sensor has been successfully applied for the determination of lactose in dairy (milk with different percentages of fat, lactose-free milk and yogurt) with a good reproducibility (RSD = 1.5-2.2%). No sample preparation except a simple dilution process is needed. The biosensor is easy to make and operate, is inexpensive and reveals a high sensitivity and reliability. (C) 2009 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Multiwalled carbon nanotubes, Lactose detection, Third generation biosensor, Cross-linking, Screen-printed electrodes
in
Electrochimica Acta
volume
55
issue
26
pages
7690 - 7695
publisher
Elsevier
conference name
7th Spring Meeting of the International-Society-of-Electrochemistry on Recent Advances in Electrocatalysis and Photoelectrocatalysis
external identifiers
  • wos:000283209800021
  • scopus:77956806866
ISSN
1873-3859
0013-4686
DOI
10.1016/j.electacta.2009.10.052
language
English
LU publication?
yes
id
1a18e959-00b2-4056-a808-af9c513ced5f (old id 1720951)
date added to LUP
2010-12-06 10:56:01
date last changed
2018-07-01 03:06:37
@inproceedings{1a18e959-00b2-4056-a808-af9c513ced5f,
  abstract     = {A rapid and simple approach of lactose analysis is proposed based on 3rd generation amperometric biosensors employing cellobiose dehydrogenase (CDH) from Trametes villosa or Phanerochaete sordida immobilised on screen-printed carbon electrodes (SPCEs). After optimisation of the working conditions of the biosensors - pH of the carrier buffer, flow rate and applied potential - the sensors were able to detect lactose in a concentration range between 0.5-200 mu M and 0.5-100 mu M employing T. villosa and P. sordida CDH, respectively. The limit of detection is 250 nM (90 mu g/L) for both. Biosensors based on SPCEs modified with multiwalled carbon nanotubes showed a higher sensitivity than unmodified SPCEs. Crosslinking with glutaraldehyde or poly(ethyleneglycol)diglycidyl ether improved not only the stability but also the analytical response. The developed sensor has been successfully applied for the determination of lactose in dairy (milk with different percentages of fat, lactose-free milk and yogurt) with a good reproducibility (RSD = 1.5-2.2%). No sample preparation except a simple dilution process is needed. The biosensor is easy to make and operate, is inexpensive and reveals a high sensitivity and reliability. (C) 2009 Elsevier Ltd. All rights reserved.},
  author       = {Safina, Gulnara and Ludwig, Roland and Gorton, Lo},
  booktitle    = {Electrochimica Acta},
  issn         = {1873-3859},
  keyword      = {Multiwalled carbon nanotubes,Lactose detection,Third generation biosensor,Cross-linking,Screen-printed electrodes},
  language     = {eng},
  number       = {26},
  pages        = {7690--7695},
  publisher    = {Elsevier},
  title        = {A simple and sensitive method for lactose detection based on direct electron transfer between immobilised cellobiose dehydrogenase and screen-printed carbon electrodes},
  url          = {http://dx.doi.org/10.1016/j.electacta.2009.10.052},
  volume       = {55},
  year         = {2010},
}