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Effect of Heparin, Fucoidan and Other Polysaccharides on Adhesion of Enterohepatic Helicobacter Species to Murine Macrophages.

Lutay, Nataliya LU ; Nilsson, Ingrid LU ; Wadström, Torkel LU and Ljungh, Åsa LU (2011) In Applied Biochemistry and Biotechnology 164. p.1-9
Abstract
Helicobacter species have been isolated and cultured from both the gastric and enterohepatic niches of the gastrointestinal tract and are associated with a wide spectrum of diseases. Some members of the enterohepatic Helicobacter species (EHS), which include Helicobacter bilis, Helicobacter hepaticus and Helicobacter pullorum, are associated with chronic inflammatory and proliferative bowel inflammation, hepatitis and in experimental murine studies with hepatic cancer. The present study aimed to explore if polysulphated polysaccharides can prevent adhesion of EHS to the murine macrophage cell line J774A.1. A competitive binding assay showed that heparin and heparan sulphate at a concentration of 1.25 mg/ml reduced binding of H. hepaticus... (More)
Helicobacter species have been isolated and cultured from both the gastric and enterohepatic niches of the gastrointestinal tract and are associated with a wide spectrum of diseases. Some members of the enterohepatic Helicobacter species (EHS), which include Helicobacter bilis, Helicobacter hepaticus and Helicobacter pullorum, are associated with chronic inflammatory and proliferative bowel inflammation, hepatitis and in experimental murine studies with hepatic cancer. The present study aimed to explore if polysulphated polysaccharides can prevent adhesion of EHS to the murine macrophage cell line J774A.1. A competitive binding assay showed that heparin and heparan sulphate at a concentration of 1.25 mg/ml reduced binding of H. hepaticus and H. pullorum to the host cells, but not H. bilis. Of the tested Helicobacter spp, the highest inhibition by heparin was demonstrated for H. pullorum (P < 0.01), the most hydrophilic strain. Partially or completely de-sulphated heparin derivatives lost the ability to inhibit adherence of EHS, indicating the importance of sulphated groups of heparin. The most efficient inhibitor of EHS binding to macrophages was fucoidan, which reduced bacterial adhesion of the three enterohepatic Helicobacter species to a greater extent than heparin, 60-90% inhibition vs 30-70% inhibition by heparin. Identification of receptors that EHS ligands bind to is important for understanding the development of infection and may provide a rational target to prevent infection and therapy. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Biochemistry and Biotechnology
volume
164
pages
1 - 9
publisher
Humana Press
external identifiers
  • wos:000288547300001
  • pmid:21088929
  • scopus:79957446172
ISSN
1559-0291
DOI
10.1007/s12010-010-9109-7
language
English
LU publication?
yes
id
a586437e-751e-49ee-98eb-f5d9214f4f63 (old id 1731801)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21088929?dopt=Abstract
date added to LUP
2010-12-01 12:40:36
date last changed
2017-09-17 04:00:17
@article{a586437e-751e-49ee-98eb-f5d9214f4f63,
  abstract     = {Helicobacter species have been isolated and cultured from both the gastric and enterohepatic niches of the gastrointestinal tract and are associated with a wide spectrum of diseases. Some members of the enterohepatic Helicobacter species (EHS), which include Helicobacter bilis, Helicobacter hepaticus and Helicobacter pullorum, are associated with chronic inflammatory and proliferative bowel inflammation, hepatitis and in experimental murine studies with hepatic cancer. The present study aimed to explore if polysulphated polysaccharides can prevent adhesion of EHS to the murine macrophage cell line J774A.1. A competitive binding assay showed that heparin and heparan sulphate at a concentration of 1.25 mg/ml reduced binding of H. hepaticus and H. pullorum to the host cells, but not H. bilis. Of the tested Helicobacter spp, the highest inhibition by heparin was demonstrated for H. pullorum (P &lt; 0.01), the most hydrophilic strain. Partially or completely de-sulphated heparin derivatives lost the ability to inhibit adherence of EHS, indicating the importance of sulphated groups of heparin. The most efficient inhibitor of EHS binding to macrophages was fucoidan, which reduced bacterial adhesion of the three enterohepatic Helicobacter species to a greater extent than heparin, 60-90% inhibition vs 30-70% inhibition by heparin. Identification of receptors that EHS ligands bind to is important for understanding the development of infection and may provide a rational target to prevent infection and therapy.},
  author       = {Lutay, Nataliya and Nilsson, Ingrid and Wadström, Torkel and Ljungh, Åsa},
  issn         = {1559-0291},
  language     = {eng},
  pages        = {1--9},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Effect of Heparin, Fucoidan and Other Polysaccharides on Adhesion of Enterohepatic Helicobacter Species to Murine Macrophages.},
  url          = {http://dx.doi.org/10.1007/s12010-010-9109-7},
  volume       = {164},
  year         = {2011},
}