Studies of proteoglycan involvement in CPP-mediated delivery.
(2011) In Methods in Molecular Biology 683. p.99-115- Abstract
- Cell-penetrating peptides (CPPs) are widely used to deliver macromolecular cargoes to intracellular sites of action. Many CPPs have been demonstrated to rely on cell surface heparan sulfate proteoglycans (HSPGs) for efficient cellular entry and delivery. In this chapter, we describe methods for the study of PG involvement in CPP uptake. We provide descriptions of how to determine whether uptake of a CPP of interest is dependent on PGs. We also provide detailed protocols for the purification of PGs by anion-exchange chromatography as well as the characterization of the HSPG core protein composition of a cell line of interest. Finally, we present methods for modulating the expression level of specific HSPG core proteins as a means to... (More)
- Cell-penetrating peptides (CPPs) are widely used to deliver macromolecular cargoes to intracellular sites of action. Many CPPs have been demonstrated to rely on cell surface heparan sulfate proteoglycans (HSPGs) for efficient cellular entry and delivery. In this chapter, we describe methods for the study of PG involvement in CPP uptake. We provide descriptions of how to determine whether uptake of a CPP of interest is dependent on PGs. We also provide detailed protocols for the purification of PGs by anion-exchange chromatography as well as the characterization of the HSPG core protein composition of a cell line of interest. Finally, we present methods for modulating the expression level of specific HSPG core proteins as a means to determine the core protein specificity in the uptake of a particular CPP. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1732279
- author
- Wittrup, Anders LU ; Zhang, Sihe and Belting, Mattias LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Methods in Molecular Biology
- volume
- 683
- pages
- 99 - 115
- publisher
- Springer
- external identifiers
-
- pmid:21053125
- scopus:79952202646
- pmid:21053125
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-60761-919-2_8
- language
- English
- LU publication?
- yes
- id
- 6874a296-afd7-4054-8511-0c93015df8a2 (old id 1732279)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21053125?dopt=Abstract
- date added to LUP
- 2016-04-04 08:56:49
- date last changed
- 2022-01-29 08:01:10
@article{6874a296-afd7-4054-8511-0c93015df8a2,
abstract = {{Cell-penetrating peptides (CPPs) are widely used to deliver macromolecular cargoes to intracellular sites of action. Many CPPs have been demonstrated to rely on cell surface heparan sulfate proteoglycans (HSPGs) for efficient cellular entry and delivery. In this chapter, we describe methods for the study of PG involvement in CPP uptake. We provide descriptions of how to determine whether uptake of a CPP of interest is dependent on PGs. We also provide detailed protocols for the purification of PGs by anion-exchange chromatography as well as the characterization of the HSPG core protein composition of a cell line of interest. Finally, we present methods for modulating the expression level of specific HSPG core proteins as a means to determine the core protein specificity in the uptake of a particular CPP.}},
author = {{Wittrup, Anders and Zhang, Sihe and Belting, Mattias}},
issn = {{1940-6029}},
language = {{eng}},
pages = {{99--115}},
publisher = {{Springer}},
series = {{Methods in Molecular Biology}},
title = {{Studies of proteoglycan involvement in CPP-mediated delivery.}},
url = {{http://dx.doi.org/10.1007/978-1-60761-919-2_8}},
doi = {{10.1007/978-1-60761-919-2_8}},
volume = {{683}},
year = {{2011}},
}