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Studies of proteoglycan involvement in CPP-mediated delivery.

Wittrup, Anders LU ; Zhang, Sihe and Belting, Mattias LU (2011) In Methods in Molecular Biology 683. p.99-115
Abstract
Cell-penetrating peptides (CPPs) are widely used to deliver macromolecular cargoes to intracellular sites of action. Many CPPs have been demonstrated to rely on cell surface heparan sulfate proteoglycans (HSPGs) for efficient cellular entry and delivery. In this chapter, we describe methods for the study of PG involvement in CPP uptake. We provide descriptions of how to determine whether uptake of a CPP of interest is dependent on PGs. We also provide detailed protocols for the purification of PGs by anion-exchange chromatography as well as the characterization of the HSPG core protein composition of a cell line of interest. Finally, we present methods for modulating the expression level of specific HSPG core proteins as a means to... (More)
Cell-penetrating peptides (CPPs) are widely used to deliver macromolecular cargoes to intracellular sites of action. Many CPPs have been demonstrated to rely on cell surface heparan sulfate proteoglycans (HSPGs) for efficient cellular entry and delivery. In this chapter, we describe methods for the study of PG involvement in CPP uptake. We provide descriptions of how to determine whether uptake of a CPP of interest is dependent on PGs. We also provide detailed protocols for the purification of PGs by anion-exchange chromatography as well as the characterization of the HSPG core protein composition of a cell line of interest. Finally, we present methods for modulating the expression level of specific HSPG core proteins as a means to determine the core protein specificity in the uptake of a particular CPP. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
683
pages
99 - 115
publisher
Springer
external identifiers
  • pmid:21053125
  • scopus:79952202646
ISSN
1940-6029
DOI
10.1007/978-1-60761-919-2_8
language
English
LU publication?
yes
id
6874a296-afd7-4054-8511-0c93015df8a2 (old id 1732279)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21053125?dopt=Abstract
date added to LUP
2010-12-01 10:54:08
date last changed
2017-01-01 07:41:30
@article{6874a296-afd7-4054-8511-0c93015df8a2,
  abstract     = {Cell-penetrating peptides (CPPs) are widely used to deliver macromolecular cargoes to intracellular sites of action. Many CPPs have been demonstrated to rely on cell surface heparan sulfate proteoglycans (HSPGs) for efficient cellular entry and delivery. In this chapter, we describe methods for the study of PG involvement in CPP uptake. We provide descriptions of how to determine whether uptake of a CPP of interest is dependent on PGs. We also provide detailed protocols for the purification of PGs by anion-exchange chromatography as well as the characterization of the HSPG core protein composition of a cell line of interest. Finally, we present methods for modulating the expression level of specific HSPG core proteins as a means to determine the core protein specificity in the uptake of a particular CPP.},
  author       = {Wittrup, Anders and Zhang, Sihe and Belting, Mattias},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {99--115},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Studies of proteoglycan involvement in CPP-mediated delivery.},
  url          = {http://dx.doi.org/10.1007/978-1-60761-919-2_8},
  volume       = {683},
  year         = {2011},
}